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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Takara) > PrimeScript¢â Reverse Transcriptase
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ standard

PrimeScript¢â Reverse Transcriptase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2680A
PrimeScript¢â Reverse Transcriptase
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
10,000 U
178,400¿ø 
223,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2680A_DS.v1901Da.pdf
Takara
2680B
PrimeScript¢â Reverse Transcriptase
°ü·ÃÇмú ±¸¸ÅÇϱâ
40,000 U (2680A x 4)
800,000¿ø 



  • High fidelity·Î ³ôÀº Á¤È®µµÀÇ cDNA ÇÕ¼º, Cloning ½ÇÇè¿¡ ÃÖÀû
  • Strong strand displacement & elongation activity·Î Full length cDNA ÇÕ¼º¿¡ ÃÖÀû (~ 12 kb)
  • ³·Àº ¹ÝÀÀ¿Âµµ (42¡É)¿¡¼­ GC-rich ¹× °íÂ÷±¸Á¶ RNA »ùÇñîÁö Àû¿ë (RNAÀÇ degradation ÃÖ¼ÒÈ­)
  • ³ôÀº ƯÀ̼º, ³ôÀº sensitivity·Î non-specific annealing ¾øÀÌ, ¼Ò·® RNA »ùÇÿ¡ Àû¿ë °¡´É
Á¦Ç°¼³¸í
ÀÌ Á¦Ç°Àº M-MLV(Moloney Murine Leukemia virus) À¯·¡ÀÇ Reverse Transcriptase¸¦ ¹ÙÅÁÀ¸·Î °³¹ßÇÑ RNase H minusÀÇ ¿ªÀü»ç È¿¼ÒÀÌ´Ù. cDNA ÇÕ¼ºÀÇ ÃÖ´ë ÀúÇØ¿äÀÎÀÎ È¿¼ÒÀÇ non-specific annealing°ú mis-priming¿¡ ÀÇÇÑ ºñƯÀÌÀû cDNA ÇÕ¼ºÀ» ¾ïÁ¦Çϵµ·Ï µðÀÚÀεǾú´Ù.
ÀÌ µÎ°¡Áö Ư¡°ú °­ÇÑ displacement activity·Î 42°CÀÇ ¹ÝÀÀ ¿Âµµ¿¡¼­ RNA ¼Õ»ó ¾øÀÌ º¹ÀâÇÑ ±¸Á¶¿Í ±æÀÌ°¡ ±ä RNA¿¡ ´ëÇؼ­µµ ¸Å¿ì ³·Àº background·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÏ´Ù. ÀÌ È¿¼Ò´Â ±æÀÌ°¡ ±ä cDNAÀÇ ÇÕ¼ºÀ̳ª full length cDNAÀÇ ºñÀ²ÀÌ ³ôÀº library¸¦ Á¦ÀÛÇÒ ¶§ ÃÖÀûÀÌ´Ù.
º¸Á¸ -20¡É
³óµµ 200 U/§¡
Çü»ó

Tris-HCl, pH7.8

 

20 mM

NaCl

 

100 mM

EDTA

 

1 mM

DTT

 

1 mM

Glycerol (v/v)

 

50%

5¡¿ PrimeScript Buffer Á¶¼º

Tris-HCl (pH8.3)

 

250 mM

KCl

 

375 mM

MgCl2

 

15 mM

È°¼ºÀÇ Á¤ÀÇ
- 37°C¿¡¼­ 10ºÐ µ¿¾È Poly(rA)·oligo(dT) 12-18À» template ¹× primer·Î »ç¿ëÇÏ¿© 1nmolÀÇ [3H]dTTP¸¦ DNA·Î ÇÕ¼ºÇÏ´Â È¿¼ÒÀÇ ¾çÀ» 1UÀ¸·Î ÇÑ´Ù.
È°¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

50 mM

 

Tris-HCl, pH8.3

75 mM

 

KCl

8 mM

 

MgCl2

10 mM

 

DTT

20 §¶/§¡

 

(rA)n(dT)12-18

0.5 mM

 

[3H]dTTP

0.1%

 

NP-40

±â¿ø
E.coli·ÎºÎÅÍ ¹ßÇö, ºÐ¸® ¹× Á¤Á¦µÈ ÀçÁ¶ÇÕ ´Ü¹éÁú
¿ëµµ
  • ´ÜÀÏ °¡´Ú cDNA ÇÕ¼º
  • cDNA probe Á¦ÀÛ
  • RT-PCR
³ôÀº Á¤È®µµÀÇ ¿ªÀü»ç È¿¼Ò
°ü·ÃÁ¦Ç°
- High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
- Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø High fidelity ¿ªÀü»ç È¿¼Ò standard, PrimeScript™ 1st strand cDNA Synthesis Kit (Code 6110A)
- ¾Ç¼¼¼­¸® ´Ü¹éÁú·Î °íÂ÷±¸Á¶ RNA Àû¿ë¼ºÀÌ °­È­µÈ PrimeScript™, PrimeScript™ II 1st strand cDNA Synthesis Kit (Code 6210A)
- gDNA Á¦°Å ÈÄ Real-Time PCR¿ë cDNA ÇÕ¼º, PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Code RR047A)

Keyword : 2640A

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