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Hot Start PCR ¿ë Anti-Taq Antibody

Taq Antibody

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9002A
Taq Antibody
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
120,000¿ø 
150,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9002A_DS.v2401Da.pdf
Takara
9002B
Taq Antibody
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (9002A x 4)
536,000¿ø 

Á¦Ç° ¼³¸í
Taq Antibody´Â Hot Start PCR¿ë Anti-Taq antibodyÀÌ´Ù. Taq DNA polymerase¿Í °áÇÕÇϹǷμ­ polymerase È°¼ºÀ» ¾ïÁ¦ÇÏ¿©, PCR cycleÀ» ½ÃÀÛÇϱâ Àü¿¡ misprimingÀ̳ª primer dimer¿¡ ÀÇÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¹æÁöÇÑ´Ù. Anti-Taq antibody´Â PCR ¹ÝÀÀÀÇ Ã¹¹ø° DNA denaturation ´Ü°è¿¡¼­ º¯¼ºµÇ¹Ç·Î PCR Á¶°Ç º¯°æ¾øÀÌ ±âÁ¸ PCR Á¶°Ç¿¡¼­ »ç¿ë °¡´ÉÇϸç, TaKaRa Taq¢â, TaKaRa Ex Taq¢ç, TaKaRa LA Taq¢ç, TaKaRa Z-Taq¢â°ú È¥ÇÕÇÏ¿© °£´ÜÇÏ°Ô Hot Start PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
º¸Á¸ -20 ¡É
Çü»ó

22 mM

Tris-HCl (pH8.0)

100 mM

KCl

15 mM

NaCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

Nonidet P-40

50%

Glycerol

»ç¿ë ¹æ¹ý
1) Taq DNA Polymerase¿Í Taq Antibody¸¦ µ¿·® È¥ÇÕÇÏ°í 20~25¡É¿¡¼­ ¾à 10ºÐ°£ ¹æÄ¡ÇÑ´Ù (¹Ýµå½Ã ¿ø¾×À» È¥ÇÕ).
2) °¢ È¿¼ÒÁ¦Ç°ÀÇ »ç¿ë¹æ¹ý¿¡ µû¶ó PCR ¹ÝÀÀÀ» ¼öÇàÇÑ´Ù.
*»ó±â ¹æ¹ýÀ¸·Î TaKaRa Taq¢â, TaKaRa Ex Taq¢ç, TaKaRa LA Taq¢ç, TaKaRa Z-Taq¢â Á¦Ç°¿¡¼­ Taq Antibody°¡ È¿À²ÀûÀ¸·Î ÀÛ¿ëÇÏ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.
°ü·ÃÁ¦Ç°
- °íÈ¿À², °í°¨µµ, PCR È¿¼ÒÀÇ standard: TaKaRa Ex Taq¢ç
- ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö: TaKaRa Ex Taq¢ç Hot Start Version
- Premix ŸÀÔÀÇ Hot start ¹öÀü: Premix Ex Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword :

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)