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PCR
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º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
> TaKaRa źÀú±Õ PCR Detection Kit
TaKaRa źÀú±Õ PCR Detection Kit
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Takara
RR027
TaKaRa źÀú±Õ PCR Detection Kit
PA, CAP µ¿½Ã °ËÃâ 48 ȸ
526,400¿ø
658,000¿ø
09.02 ~ 10.31
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
³»¿ë (48 ȸ)
TaKaRa Ex Taq HS (5 unit/ §¡)
12.5 §¡
5¡¿Reaction Mixture
*
500 §¡
PA Primers (PA7, PA6)(°¢ 10 ¥ìM)
200 §¡
CAP Primers (M011, M012) (°¢ 10 ¥ìM)
200 §¡
100 bp DNA Ladder (650 ng / 5 §¡)
50 §¡
6¡¿ Loading buffer
**
60 §¡
*
dNTP Mixture, Internal Control ÇÔÀ¯.
**
36% Glycerol, 30 mM EDTA, 0.06% Bromophenol Blue, 0.06% Xylene cyanol
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źÀú±ÕÀº Æ÷ÀÚ¸¦ Çü¼ºÇϴ ȣ±â¼º ±×·¥¾ç¼º°£±ÕÀ¸·Î µÎ Á¾·ù ÀÇ µ¶¼º Çö󽺹̵å (pX01, pX02)¿¡ ÀÇÇØ º´¿ø¼ºÀ» ³ªÅ¸³½´Ù. pX01 plasmid´Â ¼¼ Á¾·ùÀÇ µ¶¼Ò ¼ººÐ (PA£ºprotective antigen, LF£ºlethal factor, EF£ºedema factor)À» ¾ÏÈ£ÈÇÏ°í ÀÖÀ¸¸ç, pX02 Çö󽺹̵å´Â Çù¸·ÇÕ¼º¿¡ °ü¿©ÇÏ´Â À¯ÀüÀÚ (capA, capB, capC)¸¦ ¾ÏÈ£ÈÇÏ°í ÀÖ´Ù. ÀÌ»óÀÇ 2Á¾·ù plasmid¸¦ ¸ðµÎ °®°í ÀÖ ´Â °æ¿ì¿¡ º´¿ø¼ºÀ» ³ªÅ¸³½´Ù. TaKaRa źÀú±Õ PCR Detection Kit´Â pX01 plasmid°¡ °®°í ÀÖ´Â PAÀ¯ÀüÀÚ ¹× pX02 plasmid°¡ °®°íÀÖ´Â capA À¯ÀüÀÚ¸¦ PCR¹ýÀ» ÀÌ¿ëÇÏ¿© °ËÃâÇÑ´Ù. ÁõÆø¿¡´Â Hot Start PCR¿ë È¿¼Ò (TaKaRa Ex Taq HS)¸¦ »ç¿ëÇÏ°í ÀÖÀ¸¹Ç·Î ½ÃÀÛÀü¿¡ misprimingÀ̳ª primer dimer Çü¼ºÀ» ÁÙÀÏ ¼ö ÀÖ¾î ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÏ¿© °í°¨µµÀÇ °ËÃâÀÌ °¡´ÉÇÏ´Ù. ¶Ç internal controlÀÌ Æ÷ÇԵǾî ÀÖ¾î false negativeÀ» ¸ð´ÏÅ͸µÇÒ ¼ö ÀÖ´Ù. º» Å°Æ®´Â ´Ü½Ã°£¿¡ °ËÃâÀÌ °¡´ÉÇÑ °£ÀÌ °ËÃâ Å°Æ®ÀÌ´Ù. źÀú±ÕÀÇ ÃÖÁ¾ ÆÇ´ÜÀº º» Å°Æ®ÀÇ »ç¿ë»Ó¸¸ ¾Æ´Ï¶ó ±×·¥¿°»ö µîÀ» Æ÷ÇÔÇÑ ÀϹÝÀûÀÎ ¹Ì»ý¹°ÇÐÀû ¹æ¹ýÀ» ÇÔ²² »ç¿ëÇÒ°ÍÀ» ±ÇÀåÇÑ´Ù.
ÇÁ¶óÀÌ¸Ó ¼¿ ¹× ÁõÆø±æÀÌ
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Target product ±æÀÌ
Internal control ±æÀÌ
PA Primers
PA7
5¡¯-ATCAC CAGAG GCAAG ACACC C-3¡¯
211 bp
409 bp
PA6
5¡¯-ACCAA TATCA AAGAA CGACG C-3¡¯
CAP Primers
MO11
5¡¯-GACGG ATTAT GGTGC TAAG-3¡¯
591 bp
98 bp
MO12
5¡¯-GCACT GGCAA CTGGT TTTG-3¡¯
License Notice : [L15, M57, P1]
Keyword :
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë Primer Set
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë Primer Set
Àå¿° Vibrio °ËÃâ¿ë Primer Set
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Primer Set VCT-1 & 2
½ÄÁßµ¶ °Ë»ç¿ë primer set¿Í ÇÔ²² »ç¿ëÇÏ´Â
Ư¼ö ¼¼±Õ °ËÃâ¿ë Positive Control Template
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë Primer Set
PCR »ê¹° Sequence Çؼ®À¸·Î ¼¼±ÕÀ» ÃßÁ¤
Bacterial 16S rDNA PCR Kit
Bacterial 16S rDNA PCR Kit Fast
Botulinus±Õ °ËÃâ¿ë Primer Set
Fungal rDNA (ITS1) PCR Kit Fast
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ)One Shot PCR Screening Kit Ver.2
Salmonella±Õ °ËÃâ¿ë Primer Set
Shigella ¹× Àå°üħÀÔ¼º ´ëÀå±Õ EIEC£©°ËÃâ¿ë Primer Set
TaKaRa źÀú±Õ PCR Detection Kit
Nested PCR·Î Mycoplasma Á¤¹Ð°ËÃâ
TaKaRa PCR Mycoplasma Detection Set
Welchii±Õ °ËÃâ¿ë Primer Set CPE-1 & 2