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Home > ÀüÁ¦Ç°º¸±â > PCR > º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR) > TaKaRa źÀú±Õ PCR Detection Kit

TaKaRa źÀú±Õ PCR Detection Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR027
TaKaRa źÀú±Õ PCR Detection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
PA, CAP µ¿½Ã °ËÃâ 48 ȸ
526,400¿ø 
658,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR027_e.v1706Da.pdf

³»¿ë (48 ȸ)
TaKaRa Ex Taq HS (5 unit/ §¡) 12.5 §¡
5¡¿Reaction Mixture* 500 §¡
PA Primers (PA7, PA6)(°¢ 10 ¥ìM) 200 §¡
CAP Primers (M011, M012) (°¢ 10 ¥ìM) 200 §¡
100 bp DNA Ladder (650 ng / 5 §¡) 50 §¡
6¡¿ Loading buffer ** 60 §¡
* dNTP Mixture, Internal Control ÇÔÀ¯.
** 36% Glycerol, 30 mM EDTA, 0.06% Bromophenol Blue, 0.06% Xylene cyanol
º¸Á¸ -20¡É
Á¦Ç°¼³¸í
źÀú±ÕÀº Æ÷ÀÚ¸¦ Çü¼ºÇϴ ȣ±â¼º ±×·¥¾ç¼º°£±ÕÀ¸·Î µÎ Á¾·ù ÀÇ µ¶¼º Çö󽺹̵å (pX01, pX02)¿¡ ÀÇÇØ º´¿ø¼ºÀ» ³ªÅ¸³½´Ù. pX01 plasmid´Â ¼¼ Á¾·ùÀÇ µ¶¼Ò ¼ººÐ (PA£ºprotective antigen, LF£ºlethal factor, EF£ºedema factor)À» ¾ÏȣȭÇÏ°í ÀÖÀ¸¸ç, pX02 Çö󽺹̵å´Â Çù¸·ÇÕ¼º¿¡ °ü¿©ÇÏ´Â À¯ÀüÀÚ (capA, capB, capC)¸¦ ¾ÏȣȭÇÏ°í ÀÖ´Ù. ÀÌ»óÀÇ 2Á¾·ù plasmid¸¦ ¸ðµÎ °®°í ÀÖ ´Â °æ¿ì¿¡ º´¿ø¼ºÀ» ³ªÅ¸³½´Ù. TaKaRa źÀú±Õ PCR Detection Kit´Â pX01 plasmid°¡ °®°í ÀÖ´Â PAÀ¯ÀüÀÚ ¹× pX02 plasmid°¡ °®°íÀÖ´Â capA À¯ÀüÀÚ¸¦ PCR¹ýÀ» ÀÌ¿ëÇÏ¿© °ËÃâÇÑ´Ù. ÁõÆø¿¡´Â Hot Start PCR¿ë È¿¼Ò (TaKaRa Ex Taq HS)¸¦ »ç¿ëÇÏ°í ÀÖÀ¸¹Ç·Î ½ÃÀÛÀü¿¡ misprimingÀ̳ª primer dimer Çü¼ºÀ» ÁÙÀÏ ¼ö ÀÖ¾î ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÏ¿© °í°¨µµÀÇ °ËÃâÀÌ °¡´ÉÇÏ´Ù. ¶Ç internal controlÀÌ Æ÷ÇԵǾî ÀÖ¾î false negativeÀ» ¸ð´ÏÅ͸µÇÒ ¼ö ÀÖ´Ù. º» Å°Æ®´Â ´Ü½Ã°£¿¡ °ËÃâÀÌ °¡´ÉÇÑ °£ÀÌ °ËÃâ Å°Æ®ÀÌ´Ù. źÀú±ÕÀÇ ÃÖÁ¾ ÆÇ´ÜÀº º» Å°Æ®ÀÇ »ç¿ë»Ó¸¸ ¾Æ´Ï¶ó ±×·¥¿°»ö µîÀ» Æ÷ÇÔÇÑ ÀϹÝÀûÀÎ ¹Ì»ý¹°ÇÐÀû ¹æ¹ýÀ» ÇÔ²² »ç¿ëÇÒ°ÍÀ» ±ÇÀåÇÑ´Ù.
ÇÁ¶óÀÌ¸Ó ¼­¿­ ¹× ÁõÆø±æÀÌ
ÇÁ¶óÀÌ¸Ó ¼­¿­ Target product ±æÀÌ Internal control ±æÀÌ
PA Primers
PA7 5¡¯-ATCAC CAGAG GCAAG ACACC C-3¡¯ 211 bp 409 bp
PA6 5¡¯-ACCAA TATCA AAGAA CGACG C-3¡¯
CAP Primers
MO11 5¡¯-GACGG ATTAT GGTGC TAAG-3¡¯ 591 bp 98 bp
MO12 5¡¯-GCACT GGCAA CTGGT TTTG-3¡¯
License Notice : [L15, M57, P1]

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