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Home > ÀüÁ¦Ç°º¸±â > PCR > RT-PCR °ü·Ã Á¦Ç° > PrimeScript¢â RT-PCR Kit
PrimeScript RTase £¦ TaKaRa Ex Taq HS·Î ÃÖÀûÈ­µÈ 2 Step RT-PCR Kit

PrimeScript¢â RT-PCR Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR014A
PrimeScript¢â RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
256,800¿ø 
321,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR014A_e.v2108Da.pdf
Takara
RR014B
PrimeScript¢â RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (RR014A x 4)
1,152,000¿ø 

´ÙÄ«¶ó ÃÖ½ÅÀÇ ¿ªÀü»ç È¿¼ÒÀÎ PrimeScript RTase¿Í TaKaRa Ex TaqÀÇ Á¶ÇÕÀ» ÀÌ¿ëÇÑ 2step RT-PCR Kit ÀÔ´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ¶Ù¾î³­ ½ÅÀ强°ú ¸Å¿ì ³ôÀº ÁõÆø È¿À²À» Á¦°øÇÏ´Â 2 step RT-PCR kitÀÌ´Ù. ¿ªÀü»ç ¹ÝÀÀ¿¡´Â M-MLV À¯·¡ÀÇ ¿ªÀü»ç È¿¼Ò¸¦ Åä´ë·Î TaKaRa Bio°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ½Å±Ô ¿ªÀü»ç È¿¼Ò PrimeScript RTase¸¦ ä¿ëÇß´Ù.

¶ÇÇÑ, PCR¿¡´Â ÁõÆø È¿À²ÀÌ ¶Ù¾î³­ Hot Start PCR È¿¼Ò TaKaRa Ex Taq HS¸¦ »ç¿ëÇß´Ù.

º» Kit´Â ´ÙÀ½°ú °°Àº Ư¡ÀÌ ÀÖ´Ù.

  • È¿À²ÀûÀ¸·Î RT-PCR ÁõÆø »ê¹°À» ¾òÀ» ¼ö ÀÖ´Ù.
  • Ç¥ÁØÀûÀÎ ¿ªÀü»ç ¹ÝÀÀ¿Âµµ (42¡É)·Î º¹ÀâÇÑ ±¸Á¶ÀÇ template RNA¿¡µµ ¶Ù¾î³­ ½ÅÀ强À» º¸ÀδÙ. RNA°¡ ºÐÇØµÉ À§Ç輺ÀÌ ÀÖ´Â °í¿Â¿¡¼­ÀÇ ¹ÝÀÀÀº ºÒÇÊ¿äÇÏ´Ù.
  • Cycle ÀüÀÇ mis-priming°ú primer dimer¿¡¼­ À¯·¡ÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÑ´Ù.
º» Kit¿¡´Â RNA·ÎºÎÅÍ cDNA ÇÕ¼º±îÁöÀÇ ¿ªÀü»ç ¹ÝÀÀ°ú PCR¿¡ ÀÇÇÑ cDNA ÁõÆø¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
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RNA template General
ÁõÆø »çÀÌÁî ÃÖ´ë 12 kb ÁõÆø È®ÀÎ
¿ªÀü»ç È¿¼Ò PrimeScript RTase (ÃÖÀû ¹ÝÀÀ¿Âµµ 42¡É)
DNA Polymerase TaKaRa Ex Taq HS
RNase Inhibitor ÇÊ¿ä (Kit¿¡ Æ÷ÇÔ)
1st strand cDNA ÇÕ¼º¿ë primer Random 6 mers, Oligo dT Primer,
Specific downstream primer Áß
¼±Åð¡´É
³»¿ë (50 ȸ) *1
PrimeScript RTase (for 2 step) 25§¡
5¡¿PrimeScript Buffer 200§¡
RNase Inhibitor (40 U/§¡) 25§¡
dNTP Mixture (10 mM each) 150§¡
Oligo dT Primer (2.5 ¥ìM) 50§¡
Random 6 mers (20 ¥ìM) 50§¡
TaKaRa Ex Taq HS (5 U/§¡) 25§¡
10¡¿PCR Buffer II 250§¡
Control F-1 Primer *2 (20 ¥ìM) 10§¡
Control R-1 Primer *3 (20 ¥ìM) 10§¡
Positive Control RNA (2¡¿105 copies/§¡) 20§¡
RNase Free dH2O 1§¢
*1 [¿ªÀü»ç ¹ÝÀÀ°è 20 §¡ ¡æ PCR ¹ÝÀÀ°è 50 §¡]ÀÎ °æ¿ì 50 ȸ ºÐ
*2 Positive Control RNA¿ë upstream sense primer
*3 Positive Control RNA¿ë downstream anti-sense primer
º¸Á¸ -20¡É

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