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PCR
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RT-PCR °ü·Ã Á¦Ç°
> PrimeScript¢â RT-PCR Kit
PrimeScript RTase £¦ TaKaRa Ex Taq HS·Î ÃÖÀûÈµÈ 2 Step RT-PCR Kit
PrimeScript¢â RT-PCR Kit
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Takara
RR014A
PrimeScript¢â RT-PCR Kit
50 ȸ
256,800¿ø
321,000¿ø
09.02 ~ 10.31
Takara
RR014B
PrimeScript¢â RT-PCR Kit
200 ȸ (RR014A x 4)
1,152,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
´ÙÄ«¶ó ÃÖ½ÅÀÇ ¿ªÀü»ç È¿¼ÒÀÎ
PrimeScript RTase
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TaKaRa Ex Taq
ÀÇ Á¶ÇÕÀ» ÀÌ¿ëÇÑ 2step RT-PCR Kit ÀÔ´Ï´Ù.
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º» Á¦Ç°Àº ¶Ù¾î³ ½ÅÀ强°ú ¸Å¿ì ³ôÀº ÁõÆø È¿À²À» Á¦°øÇÏ´Â 2 step RT-PCR kitÀÌ´Ù. ¿ªÀü»ç ¹ÝÀÀ¿¡´Â M-MLV À¯·¡ÀÇ ¿ªÀü»ç È¿¼Ò¸¦ Åä´ë·Î TaKaRa Bio°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ½Å±Ô ¿ªÀü»ç È¿¼Ò PrimeScript RTase¸¦ ä¿ëÇß´Ù.
¶ÇÇÑ, PCR¿¡´Â ÁõÆø È¿À²ÀÌ ¶Ù¾î³ Hot Start PCR È¿¼Ò TaKaRa Ex Taq HS¸¦ »ç¿ëÇß´Ù.
º» Kit´Â ´ÙÀ½°ú °°Àº Ư¡ÀÌ ÀÖ´Ù.
È¿À²ÀûÀ¸·Î RT-PCR ÁõÆø »ê¹°À» ¾òÀ» ¼ö ÀÖ´Ù.
Ç¥ÁØÀûÀÎ ¿ªÀü»ç ¹ÝÀÀ¿Âµµ (42¡É)·Î º¹ÀâÇÑ ±¸Á¶ÀÇ template RNA¿¡µµ ¶Ù¾î³ ½ÅÀ强À» º¸ÀδÙ. RNA°¡ ºÐÇØµÉ À§Ç輺ÀÌ ÀÖ´Â °í¿Â¿¡¼ÀÇ ¹ÝÀÀÀº ºÒÇÊ¿äÇÏ´Ù.
Cycle ÀüÀÇ mis-priming°ú primer dimer¿¡¼ À¯·¡ÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÑ´Ù.
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RNA template
General
ÁõÆø »çÀÌÁî
ÃÖ´ë 12 kb ÁõÆø È®ÀÎ
¿ªÀü»ç È¿¼Ò
PrimeScript RTase (ÃÖÀû ¹ÝÀÀ¿Âµµ 42¡É)
DNA Polymerase
TaKaRa Ex Taq HS
RNase Inhibitor
ÇÊ¿ä (Kit¿¡ Æ÷ÇÔ)
1st strand cDNA ÇÕ¼º¿ë primer
Random 6 mers, Oligo dT Primer,
Specific downstream primer Áß
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*1
PrimeScript RTase (for 2 step)
25§¡
5¡¿PrimeScript Buffer
200§¡
RNase Inhibitor (40 U/§¡)
25§¡
dNTP Mixture (10 mM each)
150§¡
Oligo dT Primer (2.5 ¥ìM)
50§¡
Random 6 mers (20 ¥ìM)
50§¡
TaKaRa Ex Taq HS (5 U/§¡)
25§¡
10¡¿PCR Buffer II
250§¡
Control F-1 Primer
*2
(20 ¥ìM)
10§¡
Control R-1 Primer
*3
(20 ¥ìM)
10§¡
Positive Control RNA (2¡¿105 copies/§¡)
20§¡
RNase Free dH
2
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1§¢
*1
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Positive Control RNA¿ë upstream sense primer
*3
Positive Control RNA¿ë downstream anti-sense primer
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Keyword :
RT-PCR °ü·Ã Á¦Ç°
°í¼Ó¹ÝÀÀ, ³ôÀº Á¤È®µµ ½ÇÇö - 1step RT-PCR ki..
PrimeScript¢â II High Fidelity One Step RT-PCR Kit
RT-PCR kit, ²Ä²ÄÈ÷ µûÁ®º¸°í ¼±ÅÃÇϱâ
¿ªÀü»ç ¹ÝÀÀÀÇ ÃÖÀû ¿Âµµ°¡ ³ôÀº RT-PCR ¿ë Kit
BcaBEST¢ç RNA PCR Kit Ver. 1.1
PrimeScript RTase¿Í PrimeSTAR Max¸¦ Á¶ÇÕÇÏ¿©..
PrimeScript¢â High Fidelity RT-PCR Kit
Premix·Î Æí¸®ÇÑ 1 step RT-PCR kit
PrimeScript¢â One Step RT-PCR Kit Ver.2
PrimeScript RTase £¦ TaKaRa Ex Taq HS·Î ÃÖÀû..
PrimeScript¢â RT-PCR Kit
SMART¹ýÀ» ÀÌ¿ëÇÑ new version 5¡¯-/3¡¯-RACE K..
SMARTer¢ç RACE 5'/3' Kit
PCR±îÁö 1 tube·Î °¡´ÉÇÑ 1 step RT-PCR ¿ë Kit
TaKaRa One Step RNA PCR Kit (AMV)
Long strand cDNA ÁõÆø¿¡
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
TaKaRa Ex Taq HS°ú AMV RTase¸¦ ÀÌ¿ëÇÑ 2 step..
TaKaRa RNA PCR¢â Kit (AMV) Ver.3.0