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Mutagenesis
> Diversify PCR Random Mutagenesis Kit
Error prone PCR¿¡ ÃÖÀû
Diversify PCR Random Mutagenesis Kit
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Clontech
630703
Diversify PCR Random Mutagenesis Kit
30 ȸ
1,580,000¿ø
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Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
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Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Ÿ°Ù DNA ¼¿ÀÇ mutation rateÀ» Á¤¹ÐÇÏ°Ô Á¶Àý
Hot spot ¾øÀÌ ±¤¹üÀ§ÇÑ mutation À¯µµ °¡´É
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Random mutationÀ» Á¶ÀýÇÏ¿© ´Ü¹éÁú ±â´É ¿¬±¸¿¡µµ À¯¿ë
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Diversify™ PCR Random Mutagenesis Kit´Â µ¶¼ºÀÌ ¾ø´Â ¹æ¹ýÀ» »ç¿ëÇÏ¿© ÃÖ´ë 4 kb ±æÀÌÀÇ ¼¿¿¡ random mutationÀ» À¯¹ßÇϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù.
- Diversify ¹æ¹ý
º» Á¦Ç°À» »ç¿ëÇϸé mutagenesis À¯¹ß Á¶°ÇÀ» ½±°Ô Á¶ÀýÇÒ ¼ö ÀÖ¾î¼ ¸Å¿ì ´Ù¾çÇÑ »çÀÌÁîÀÇ DNA ¼¿¿¡µµ ÃÖÀûÀÇ mutation¸¦ µµÀÔÇÒ ¼ö ÀÖ´Ù.
Mn
2+
¿Í dGTPÀÇ ¾çÀ» º¯È½ÃÅ°´Â °ÍÀ¸·Î mutation rateÀ» 1,000 bp´ç 2~8°³±îÁö ¼±ÅÃÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
Diversify ¹æ¹ý¿¡¼´Â ClontechÀÇ Titanium
Taq
PCR È¿¼Ò ½Ã½ºÅÛÀ» ÀÌ¿ëÇÏ¿© 4 kb±îÁö DNA ´ÜÆíÀ» ¾ÈÁ¤ÀûÀ¸·Î ÁõÆøÇÑ´Ù. ÀÌ¿Í °°ÀÌ ´Ù¾çÇÑ »çÀÌÁîÀÇ DNA ¼¿À» ÁõÆøÇÒ ¼ö Àֱ⠶§¹®¿¡ ªÀº DNA ´ÜÆíÀº ¹°·Ð, operon, plasmid ±×¸®°í Å©±â°¡ Å« ´Ü¹éÁúÀ» ÄÚµùÇÏ´Â ¼¿¿¡ mutationÀ» µµÀÔÇϱ⿡µµ ÀÌ»óÀûÀÌ´Ù.
Diversify™ PCR Random Mutagenesis Kit´Â
TITANIUM
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Taq
DNA Polymerase (Code 639208)
¿Í ÇÔ²² »ç¿ëÇϵµ·Ï ÃÖÀûȵǾî ÀÖÀ¸¸ç,
TaqStart™ Antibody (Code 639250)
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Taq
PCR È¿¼Ò ½Ã½ºÅÛ°ú mutationÀ» À¯¹ßÇÏ´Â buffer¿Í ½Ã¾àÀÌ ¸ðµÎ Æ÷ÇԵǾî ÀÖ´Ù.
±×¸² 1.
Buffer
Á¶¼º¿¡ µû¸¥ mutation rate Á¶Àý.
´Ù¸¥ dNTPsÀÇ ³óµµ´Â °íÁ¤ÇÑ Ã¤ Mn
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¿Í dGTPÀÇ ³óµµ¸¦ º¯È½ÃÅ´À¸·Î½á mutagenesis Á¤µµ¸¦ Á¶ÀýÇÒ ¼ö ÀÖ´Ù. Buffer 1, 5, 9¹ø¿¡¼ mutation rate´Â DNA sequencingÀ» ÅëÇØ Á÷Á¢ ÃøÁ¤ÇÏ¿´´Ù (°¢ >15,000 bp). ´Ù¸¥ mutations rateÀº Á¤·®Àû
in vivo
fidelity assayÀÇ mutagenesis level°ú DNA ¿°±â¼¿ ºÐ¼® °á°ú¸¦ ¿¬°ü½ÃÄÑ °áÁ¤ÇÏ¿´´Ù.
±×¸² 2. Diversify™ PCR Random Mutagenesis Kit¿¡ ÀÇÇÑ 4 kb ´ÜÆíÀÇ ÁõÆø.
±×¸² 1ÀÇ buffer Á¶°Ç¿¡¼ 4 kb plasmid (lane 1-9)¸¦ ÁõÆøÇÑ °á°ú¸¦ Àü±â¿µµ¿À» ÅëÇØ È®ÀÎÇÏ¿´´Ù. Å« »çÀÌÁîÀÇ ¿°±â¼¿ÀÌ Àß ÁõÆøµÈ °ÍÀ» È®ÀÎÇÏ¿´À¸¸ç, ÀÌ·ÐÀûÀ¸·Î ´õ ±ä ´ÜÆíÀÇ DNA ¿ª½Ã mutagenesis ¹× ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
³»¿ë (30 PCR rxns)
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50X Titanium
Taq
DNA Polymerase
30 §¡
10X Titanium
Taq
PCR Buffer
200 §¡
50X Diversify dNTP Mix
30 §¡
50X dNTP Mix (10 mM each)
40 §¡
dGTP (2 mM)
150 §¡
Manganese Sulfate (MnSO
4
; 8 mM)
120 §¡
Control PCR Template (~1ng/§¡)
10 §¡
Control Primer Mix (10 µM each)
10 §¡
Taq
I Restriction Enzyme (20 units/§¡)
10 §¡
PCR Grade Water
1.25 §¢
º¸Á¸
-20µµ
°ü·Ã Á¦Ç°
- 5£§-exonuclease È°¼ºÀ» Á¦°ÅÇÑ PCR È¿¼Ò :
TITANIUM
®
Taq
DNA Polymerase (Code 639208)
- Æí¸®ÇÏ°í È¿°úÀûÀÎ hot start ¹ý :
TaqStart Antibody (Code 639250)
- ¹ü¿ëÀûÀÎ agarose :
SeaKem
®
LE Agarose (Code 50004)
- °íÈ¿À²ÀÇ Ligation premix ¿Í T-vectorÀÇ Á¶ÇÕ :
Mighty TA-cloning Kit (Code 6028)
- Long strand DNAµµ methylated DNAµµ OK! °íÈ¿À² Competent Cell:
E.coli
HST08 Premium Competent Cells (Code 9128)
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â :
Clontech PCR Thermal Cycler GP
Keyword :
Mutagenesis
Error prone PCR¿¡ ÃÖÀû
Diversify PCR Random Mutagenesis Kit