50 mM

Tris-HCl (pH8.0)

1 mM

MgCl₂

0.01%

BSA

0.5 ㎎/㎖

활성화된 salmon sperm DNA

최적조건

반응조건

온도

37~42℃

4~42℃

pH

8~10

7~10

Na⁺, K⁺

0~100 mM

0~300 mM

Mg²⁺

1mM

0.01mM

DTT

0~50 mM

0 mM

2-mercaptoethanol

0~200 mM

0 mM

Sodium deoxycholate

0~0.1%

0~1%

Triton X-100

0~1%

0~1%