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´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xTractor Buffer & Buffer Kit

xTractor Buffer Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635623
xTractor Buffer Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
(Package)
765,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33127, x33204, x33205, x33206, x33207, x33208
Clontech
635656
xTractor Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
279,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33127, x33204, x33205, x33206, x33207, x33208
Clontech
635671
xTractor Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ml
371,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635625
xTractor Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ml x 2 (635671 x 2)
705,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

Affinity-tagged protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë Lysis buffer
  • ºü¸£°í ½¬¿î ½ÇÇè°úÁ¤ - 10 ºÐ ¹ÝÀÀÀ̸é ÃæºÐ
  • »ý¹°ÇÐÀû È°¼ºÀ» À¯ÁöÇÒ¼ö ÀÖ´Â nondenaturing extraction
  • ¸ðµç IMAC Resin¿¡ ÀûÇÕÇÏ¸ç ºü¸¥ his-tagged proteinÀÇ Á¤Á¦¸¦ ½ÇÇö
  • ¹ü¿ëÀû- ´Ù¾çÇÑ scaleÀÇ ´Ü¹éÁú ÃßÃâ°ú ´Ù¾çÇÑ tag (his-, GST-, FLAG-, HA-tagged protein°ú ÇÔ²² »ç¿ë)¿¡ »ç¿ë °¡´É
  • Bacterial, yeast, mammalian, ¶Ç´Â baculovirus-infected cells·ÎºÎÅÍ protein ÃßÃâ
  • SonicationÀ̳ª ´Ù¸¥ cell lysis bufferº¸´Ù ´õ È¿°úÀû

´Ù¾çÇÑ ºÐÀÚ·®ÀÇ affinity-tagged proteinÀ» Á¤Á¦Çϱâ À§ÇÏ¿© mammalian cell pellet extraction »Ó¸¸ ¾Æ´Ï¶ó bacteria¿¡µµ TALON xTractor Buffer¸¦ »ç¿ë °¡´ÉÇÏ´Ù. ¶ÇÇÑ TALON xTractor Buffer Kit¿¡´Â lysozyme°ú DNase°¡ Æ÷ÇԵǾî ÀÖ¾î bacteria ¼¼Æ÷º®°ú ¸·À» ¿ÏÀüÈ÷ Æı«ÇÒ ¼ö Àֱ⠶§¹®¿¡ ³ôÀº ºÐÀÚ·®ÀÇ ´Ü¹éÁúÀ» È¿À²ÀûÀ¸·Î ÃßÃâ °¡´É ÇÏ´Ù. º» Á¦Ç°Àº inclusion body¿¡¼­ ºÒ¿ëÇؼº ´Ü¹éÁúÀ» ÃßÃâÇϱâ À§ÇØ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Protein Extraction with xTractor Buffer than Sonication

Extraction
Method

Fraction

Protein
(mg)

Activity

Results

Sonication

Total lysate

28.6

872

Sonication provides lower yield & activity

Purified protein

0.6

2,115

xTractor Buffer Kit

Total lysate

122.1

2,277

xTractor provides higher yield & activity

Purified protein

0.9

3,054


For this comparison of protein extraction methods, LacZ protein was extracted from a 1.7 g pellet of E. coli cells expressing his-tagged protein. Lysates were purified on a TALON column and eluted with 150 nM imidazole. Protein activity was determined by measuring luminescence units.


Figure 1. xTractor Cell Lysis Buffer simplifies protein extraction. After extraction, you can purify your protein with either a standard TALON Resin column after centrifugation or filtration (left), or with the TALON CellThru Column (right).

xTractor Cell Lysis Buffer´Â his-tagged proteins ÃßÃâ¿¡ ÃÖÀûÈ­ µÇ¾î Àֱ⠶§¹®¿¡, TALON Co Resin À̳ª His60 Ni Superflow Resin ¶Ç´Â ´Ù¸¥ Á¾·ùÀÇ affinity resin¿¡µµ »ç¿ë ÇÒ ¼ö ÀÖ´Ù.

¸ðµç Á¾·ùÀÇ ´Ü¹éÁú Á¤Á¦½Ã ProteoGuard Protease Inhibitor Cocktail °ú ÇÔ²² »ç¿ëÇÒ °ÍÀ» ÃßõÇÑ´Ù.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
ÁÖ¿ä »ùÇÿ¡ µû¸¥ xTractor Buffer Lysis Protocol

Àû¿ë»ùÇà Á¾

Protocol (Ŭ¸¯ÇÏ¿© ´Ù¿î·ÎµåÇϼ¼¿ä)

xTractor Buffer Ç¥ÁØ ¸Å´º¾ó

xTractor Buffer and xTractor Buffer Kit User Manual 

Bacteria

xTractor Buffer Bacterial Sample Preparation Protocol-At-A-Glance 

Baculovirus

xTractor Buffer Baculovirus Sample Preparation Protocol-At-A-Glance 

Mammalian

xTractor Buffer Mammalian Sample Preparation Protocol-At-A-Glance 

Yeast

xTractor Buffer Yeast Sample Preparation Protocol-At-A-Glance 


Keyword : extraction,´Ü¹éÁúÁ¤Á¦,his-tag,histag,´Ü¹éÁú ÃßÃâ

His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
>
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
>
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
>
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
>
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
>
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
>
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
>
High-throughput & microscale
His60 Ni Magnetic Beads
>
4¡É¿¡¼­ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
>
Cell lysate¿¡¼­ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
>
Prepacked spin column
TALONSpin Column