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°í¼öÀ² Ni resin & FPLC purification

His60 (Nickel) Superflow resin & cartridge

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635659
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
267,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711
Clontech
635660
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ml
503,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635661
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ml¡¿4 (635660 x 4)
1,811,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635662
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ml
3,361,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635663
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ml¡¿2 (635662 x 2)
6,386,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635664
His60 Ni Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 ml¡¿4 (635662 x 4)
12,100,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635675
His60 Ni Superflow Cartridges
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿5
435,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32710
Clontech
635679
His60 Ni Superflow Cartridges
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ml¡¿5
1,111,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635674
His60 Ni Superflow Cartridge Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿5
968,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32710
Clontech
635678
His60 Ni Superflow Cartridge Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ml¡¿5
1,685,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635657
His60 Ni Gravity Columns
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿5
207,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711
Clontech
635658
His60 Ni Gravity Column Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
(Package)
718,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711
Clontech
635665
His60 Ni Buffer Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ȸ
524,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711


  • Nickel resin Áß ÃÖ°íÀÇ °áÇÕ ¼öÀ² : resin §¢ ´ç ~60 mg his-tagged protein Á¤Á¦
  • Batch/gravity flow ¿Í FPLC ÀÚµ¿È­ Á¤Á¦¿¡ ¸ðµÎ Àû¿ë °¡´É
  • ´Ù¾çÇÑ Á¤Á¦ Á¶°Ç¿¡¼­ »ç¿ë °¡´É
Á¦Ç° ¼³¸í
His60 Ni Superflow ResinÀº bacteria, mammalian ¼¼Æ÷ ¶Ç´Â baculovirus¿¡ °¨¿°µÈ ¼¼Æ÷·ÎºÎÅÍ his-tagged proteinÀ» È¿À²ÀûÀ¸·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Â °áÇÕ ¿ë·®ÀÌ Å« resinÀÌ´Ù. Modified Ni-IDA ±â¼ú·Î °áÇÕ ´É·Â, ±Ý¼Ó ÀÌ¿ÂÀÇ ´©Ãâ Á¤µµ ¹× ¼øµµ ¸é¿¡¼­ ±âÁ¸ Ni-NTA resin ¶Ç´Â ´Ù¸¥ Ni-IDA resin º¸´Ù ¶Ù¾î³­ ¼º´ÉÀ» º¸À̸ç, batch/gravity flow column, liquid chromatography system ¹× syringe processing µîÀÇ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â ´Ù¾çÇÑ ÇüÅÂÀÇ Á¦Ç°À¸·Î ÆǸŵǰí ÀÖ´Ù.

His60 Ni Superflow Cartridge´Â His60 Ni Superflow ResinÀÌ ÃæÁøµÈ pre-packed FPLC cartridge·Î AKTA³ª FPLC µîÀÇ LC systemÀ̳ª, syringe ¶Ç´Â ¸Å´º¾ó ¹æ¹ýÀ» ÀÌ¿ëÇÑ his-tagged protein Á¤Á¦¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. His60 Ni Superflow Cartridge Purification Kit ¿¡´Â His60 Ni Superflow Cartridges (1 §¢¡¿ 5 ea ¶Ç´Â 5 §¢¡¿ 5 ea)¿Í His60 Ni Buffer Set °¡ Æ÷ÇԵǾî ÀÖ¾î °íÈ¿À²·Î his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.

His60 Ni Gravity Columns Àº His60 Ni Superflow resinÀÌ ÃæÀüµÈ prepacked gravity columnÀÌ°í, His60 Ni Gravity Column Purification Kit¿¡´Â Á¤Á¦¿¡ ÇÊ¿äÇÑ His60 Ni Buffer Set ¿Í His60 Ni Gravity ColumnsÀÌ Æ÷ÇԵǾî ÀÖ´Ù.

´Ù¾çÇÑ Àû¿ë
His60 Ni Superflow ResinÀº batch/gravity flow Á¤Á¦ »Ó¸¸ ¾Æ´Ï¶ó ´ë´Ù¼öÀÇ ÀÚµ¿È­ ¾×üũ·Î¸¶Åä±×·¡ÇÇ ½Ã½ºÅÛ ¹× manual syringe Á¤Á¦¹ý¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. His60 Ni Superflow´Â IMAC¿¡¼­ °øÅëÀûÀ¸·Î ³ªÅ¸³¯ ¼ö ÀÖ´Â ±Ý¼Ó ÀÌ¿Â ´©ÃâÀ» ÃÖ¼ÒÈ­ ÇÏ¿´°í, ½ÃÆǵǴ Ni resin Áß °¡Àå ³ôÀº °áÇÕ ¿ë·®À» °¡Áö°í ÀÖÀ¸¸ç, ºü¸£°í ½±°Ô ÀçÇö¼º ÀÖ´Â Å©·Î¸¶Åä±×·¡ÇÇ Á¤Á¦¸¦ °¡´ÉÄÉ ÇÑ´Ù.
³ôÀº Á¤Á¦ È¿À²°ú ¼øµµ
His60 Ni Superflow resinÀÇ ¼º´É È®ÀÎÀ» À§ÇØ Å¸»çÀÇ Ni-NTA Superflow resin°ú ºñ±³ ½ÇÇèÀ» ÇÏ¿´´Ù. 6xHN-AcGFP1À» °¢ Á¦Á¶»çÀÇ ÇÁ·ÎÅäÄÝ¿¡ µû¶ó µ¿ÀÏÇÑ ¾çÀÇ °°Àº ½Ã·á¿¡¼­ Á¤Á¦ÇÏ¿´´Ù. ±× °á°ú, His60 Ni Superflow resinÀ» »ç¿ëÇÏ¿´À» ¶§ ´õ ¸¹Àº ¾çÀÇ ´Ü¹éÁúÀÌ °í¼øµµ·Î Á¤Á¦µÇ¾ú´Ù (±×¸² 1).


±×¸². Ÿ»ç Ni-NTA resin ´ëºñ ³ôÀº Á¤Á¦¼öÀ²°ú ´õ ³ôÀº ¼øµµ¸¦ º¸ÀÌ´Â His60 Ni Superflow resin
E. coli¿¡¼­ ¹ßÇö½ÃŲ 6xHN AcGFP1ÀÇ Á¤Á¦¸¦ À§ÇØ µ¿ÀÏÇÑ ¾çÀÇ »ùÇÃÀ» °¢°¢ His60 Ni Superflow resin (ÁÂ)°ú Ÿ»ç Ni-NTA resin (¿ì)¿¡ Àû¿ëÇÑ °á°ú His60 Ni Superflow resinÀ» ÀÌ¿ëÇÏ¿´À» ¶§ ´õ ³ôÀº ¼øµµ·Î Á¤Á¦ÇÒ ¼ö ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù (Lane FT: Flowthrough. Lanes W1 and W2: Wash. Lane E1~E4: Eluted 6xHN-AcGFP1 fractions).


±×¸². His60 Ni Superflow Resin, His60 Ni Superflow Cartridge, His60 Ni Resin Gravity Column
º¸Á¸:£º4¡É
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
°ü·ÃÁ¦Ç°
- Protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë lysis buffer : xTractor¢â Buffer Kit
- His-tagged protein °í¹ßÇöÀ» À§ÇÑ cold shock vector system : pCold¢â vector
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî
- ½Ç¿Â¿¡¼­ 30ºÐ ¿Ï·á : Western BLoT Stripping Buffer

Keyword :

His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
>
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
>
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
>
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
>
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
>
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
>
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
>
High-throughput & microscale
His60 Ni Magnetic Beads
>
4¡É¿¡¼­ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
>
Cell lysate¿¡¼­ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
>
Prepacked spin column
TALONSpin Column