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´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦
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His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
> His60 Ni Magnetic Beads
High-throughput & microscale
His60 Ni Magnetic Beads
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Clontech
635693
His60 Ni Magnetic Beads
1 ml
196,800¿ø
246,000¿ø
11.01 ~ 12.27
Clontech
635692
His60 Ni Magnetic Beads
10 mL
856,000¿ø
1,070,000¿ø
11.01 ~ 12.27
Clontech
635665
His60 Ni Buffer Set
20 ȸ
419,200¿ø
524,000¿ø
11.01 ~ 12.27
Clontech
631964
Magnetic Stand (2 tubes)
1 Set
91,200¿ø
114,000¿ø
11.01 ~ 12.27
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
His-tagged proteinÀÇ ºü¸£°í °£ÆíÇÑ ºÐ¸®
His60 Ni°ú magnetic bead¸¦ ÀÌ¿ëÇÏ¿© °í¼øµµÀÇ ´Ü¹éÁú Á¤Á¦
ÀûÀº ºÎÇÇ¿¡¼ elutionÇÏ¿© °í³óµµ Á¤Á¦ °¡´É
Á¦Ç° ¼³¸í
His60 Ni Magnetic Beads´Â ½Å¼ÓÇÏ°í °£ÆíÇÏ°Ô his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Â magnetic beads·Î, magnetic separator¸¦ ÀÌ¿ëÇØ ¹Ì¼Ò±Ô¸ðÀÇ ´Ü¹éÁúÀ» ºü¸£°í ½±°Ô ºÐ¸®ÇÒ ¼ö ÀÖ´Ù.
Highly Specific Binding & Elution
His60 Ni Magnetic Beads´Â ³ôÀº ƯÀ̼ºÀ¸·Î ÀÛÀº ºÐÀÚ·®¿¡¼ Å« ºÐÀÚ·®±îÁö ´Ù¾çÇÑ Å©±âÀÇ his-tagged protein°ú °áÇÕÇÑ´Ù. Á¤Á¦µÈ ´Ü¹éÁúÀº ¼Ò·®ÀÇ ¿ë¾× (50-200 §¡)À¸·Î elutionÇÏ¿© °í³óµµ(~3 §·/§¢)ÀÇ »ùÇÃÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. His60 Ni Magnetic Beads´Â 5% ÇöŹ¾×À¸·Î Á¦°øµÇ¸ç, §¢ ´ç 1.65§·ÀÇ ´Ü¹éÁú°ú °áÇÕÇÒ ¼ö ÀÖ´Ù.
Microscale Screening
His60 Ni Magnetic Beads¸¦ ÀÌ¿ëÇÑ microscale Á¤Á¦´Â ´Ü¹éÁúÀÇ ¹ßÇö ¼öÁØÀ» ½ºÅ©¸®´×Çϰųª protein-protein interaction ¿¬±¸¿¡ ÀûÇÕÇϸç, ¶ÇÇÑ His60 Ni ±â¼úÀÌ Á¢¸ñµÈ ClontechÀÇ His60 Ni Superflow ResinÀ» »ç¿ëÇÔÀ¸·Î½á ¿øÈ°ÇÏ°Ô ¸ñÀû ´Ü¹éÁúÀÇ Á¤Á¦¸¦ scale-up ÇÒ ¼ö ÀÖ´Ù.
±×¸². Native condition¿¡¼ÀÇ his-tagged AcGFP1 Á¤Á¦ ¿¹
AcGFP1-6xHNÀ» ¹ßÇöÇÏ´Â E. coli lysate 400 §¡¸¦ His60 Ni Magnetic Beads¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÏ¿´´Ù.
±×¸². Denaturing condition¿¡¼ÀÇ his-tagged AcGFP1 Á¤Á¦ ¿¹
AcGFP1-6xHNÀ» ¹ßÇöÇÏ´Â E. coli lysate 500 §¡¸¦ His60 Ni Magnetic Beads ¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÏ¿´´Ù.
±×¸². His60 Ni Magnetic Beads
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Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
°ü·ÃÁ¦Ç°
- Protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë lysis buffer :
xTractor¢â Buffer Kit
- His-tagged protein °í¹ßÇöÀ» À§ÇÑ cold shock vector system :
pCold¢â vector
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à :
TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ :
HRP Substrate ½Ã¸®Áî
- ½Ç¿Â¿¡¼ 30ºÐ ¿Ï·á :
Western BLoT Stripping Buffer
Keyword :
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
High-throughput & microscale
His60 Ni Magnetic Beads
4¡É¿¡¼ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
Poly Histidine tag Ç×ü
Cell lysate¿¡¼ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
Prepacked spin column
TALONSpin Column