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Positive / negative control ¿ë baculovirus

Baculovirus control virus / transfer vector

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
AB Vector
AV300
NC (negative control)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 §¢, 108 pfu/§¢
°¡°Ý¹®ÀÇ  
AB Vector
AV301
GC (green control)
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
1 §¢, 108 pfu/§¢
°¡°Ý¹®ÀÇ  
AB Vector
AV302
GCP (green control plasmid)
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 §¡, 0.2 §¶/§¡
°¡°Ý¹®ÀÇ  

Á¦Ç°¼³¸í
NC (negative control) :
Recombinant baculovirus °¨¿°¿¡ ÀÇÇÑ ´Ü¹éÁú ¹ßÇö ½ÇÇè¿¡¼­ negative control·Î »ç¿ëÇÒ ¼ö ÀÖ´Â baculovirusÀÌ´Ù.
ÀϹÝÀûÀ¸·Î baculovirus¿¡ °¨¿°µÇÁö ¾ÊÀº ¼¼Æ÷¸¦ negative control·Î ÀÌ¿ëÇÏÁö¸¸, baculovirus°¡ °¨¿°µÈ ¼÷ÁÖ ¼¼Æ÷ÀÇ ¹ßÇö ÇÁ·ÎÆÄÀÏÀ» º¯È­½ÃÅ°°Å³ª ºñ°¨¿°¼¼Æ÷¿¡¼­´Â º¼ ¼ö ¾ø´Â baculovirus À¯·¡ ´Ü¹éÁúÀÌ Á¸ÀçÇϱ⠶§¹®¿¡ ºñ°¨¿°¼¼Æ÷¸¦ negative control·Î »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÏÁö ¾Ê´Â´Ù. ¶ÇÇÑ ClontechÀÇ BacPAK6 DNA¸¦ ÀÌ¿ëÇÑ recombinant baculovirus¸¦ °¨¿°½ÃŲ ¼¼Æ÷´Â ¥â-galactosidase¸¦ ¹ßÇöÇÏ°í, wild type baculovirus´Â °¨¿°¼¼Æ÷¿¡¼­ polyhedrinÀ» ´ë·® ¹ßÇöÇϹǷΠnegative control·Î ÀûÇÕÇÏÁö ¾Ê´Ù. µû¶ó¼­ º» NC (negative control) Á¦Ç°À» baculovirus °¨¿°¿¡ ÀÇÇÑ ´Ü¹éÁú ¹ßÇö ¿¬±¸ÀÇ negative control ½ÇÇè¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.

GC (Green Control) :
GC (Green Control)´Â Aequorea Victoria À¯·¡ green-fluorescent protein (GFP)¸¦ ¹ßÇöÇÏ´Â baculovirus·Î, ¹ÙÀÌ·¯½º Áõ½ÄÀÇ ¸ð´ÏÅ͸µÀ̳ª ¹è¾ç Á¶°ÇÀÇ ÃÖÀûÈ­, ¿ª°¡ÃøÁ¤ µî¿¡ ÀÌ¿ëÇÏ´Â °æ¿ìÀÇ positive control·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.

GCP (Green Control Plasmid) :
GCP (Green Control Plasmid)´Â AcMNPV polyhedrin promoterÀÇ Á¶ÀýÇÏ¿¡ Aequorea Victoria À¯·¡ green-fluorescent protein (GFP) À¯ÀüÀÚ¸¦ ÄÚµùÇÏ°í ÀÖ´Â transfer vectorÀÌ´Ù. ProEasy ½Ã¸®Áî, GFP¸¦ Æ÷ÇÔÇÏÁö ¾Ê´Â ProFold ½Ã¸®Áî (ProFold¢â-0, ProFold-PDI, ProFold-PDI*)Clontech BacPAK6, BD BaculoGold µîÀÇ AcNPV À¯·¡ linearized baculovirus DNA¿Í ÇÔ²² °ïÃæ¼¼Æ÷ ³»·Î co-transfection ÇÏ°í polyhedrin ºÎÀ§ÀÇ homologous recombinationÀ¸·Î GFP¸¦ ¹ßÇöÇÏ´Â recombinant baculovirus¸¦ Á¦ÀÛÇÏ¿© positive control·Î ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. NC, GC, recombinant baculovirus control (BC)¸¦ °¨¿°½ÃŲ °ïÃæ ¼¼Æ÷ÀÇ ´Ü¹éÁú ºÐ¼®
°¢ baculovirus¸¦ Spodoptera frigiperda À¯·¡ Sf9 ¼¼Æ÷ °¨¿°½ÃŲ ÈÄ 60½Ã°£ ÈÄ¿¡ ȸ¼öÇÏ¿© 50 mM Tris-HCl(pH8.0), 150 mM EDTA, 0.5% NP-40À¸·Î lysisÇÏ¿©, ºÒ¿ë¼º ±¸È¹Àº 30,000¡¿g 15 ºÐ ¿ø½ÉºÐ¸®·Î Á¦°ÅÇÏ¿´´Ù. ¼ö¿ë¼º ´Ü¹éÁúÀ» 11% SDS-PAGE¿¡¼­ ºÐ¸®ÇÏ¿© Coomassie blueÀ¸·Î ¿°»öÇÏ¿´´Ù.
º¸Á¸
NC (negative control), GC (Green Control) : 4 ¡É
GCP (green control plasmid): -20 ¡É
°ü·ÃÁ¦Ç°
- gp64 protein ¿°»öÀ» ÅëÇÑ titration : BacPAK¢â Baculovirus Rapid Titer Kit
- qPCRÀ» ÀÌ¿ëÇÑ ºü¸¥ titration : BacPAK¢â qPCR Titration Kit
- Insect cell line : IPLB-Sf21 Insect Cells
- All-in-One baculovirus system : BacPAK¢â Baculovirus Expression System
- °íÈ¿À² ´Ü¹éÁú ¹ßÇöÀ» À§ÇÑ baculvirus DNA : flashBAC¢â Baculovirus Expression System
- °ïÃæ¼¼Æ÷ Àü¿ë transfection ½Ã¾à : TransIT¢ç-Insect Transfection Reagent
- ÃÊ°£´Ü His-Tagged protein Á¤Á¦ Ä÷³ : Capturem¢â His-Tagged Purification ½Ã¸®Áî (mini, maxi, plate µî)

Keyword :

°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
>
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
>
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
>
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
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Baculovirus DNA & transfer vector set
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°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
>
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
>
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>
Sf9, Sf21 cell¿¡ ÃÖÀûÈ­µÈ Transfection reage..
ProFectin
>
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ­ È¿À² Çâ»ó
ProFold Baculovirus Vectors
>
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
>
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors