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Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > ´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã > [¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦

[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦

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[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦

´Ü¹éÁú Ư¼ºÀ» È®ÀÎÇÏ´Â °¡Àå °£´ÜÇÑ ¹æ¹ýÀº Ç×ü¸¦ ÀÌ¿ëÇÏ´Â °ÍÀÌ´Ù. Ç×ü´Â ´Ü¹éÁúÀÇ ¾ç°ú º¯È­¸¦ È®ÀÎÇϰųª, ¼¼Æ÷ ³» À§Ä¡ÀÇ °áÁ¤, Àü»ç ÈÄ º¯Çü ȤÀº ´Ù¸¥ ´Ü¹éÁú°úÀÇ »óÈ£ÀÛ¿ëÀ» È®ÀÎÇÏ´Â µî¿¡ »ç¿ëµÉ ¼ö ÀÖ´Ù. ÇÏÁö¸¸, Ç×ü´Â ´Ù¾çÇÑ ´Ü¹éÁú¿¡ Àû¿ëÀÌ ºÒ°¡ÇÏ°í, »ì¾ÆÀÖ´Â ¼¼Æ÷³ª Á¶Á÷ ³»¿¡¼­ÀÇ ´Ü¹éÁú È®ÀÎÀÌ ¾î·Æ´Ù´Â ÇÑ°è°¡ ÀÖ´Ù.

´Ü¹éÁú taggingÀº ÀÌ·¯ÇÑ Ç×ü¸¦ ´ë½ÅÇÏ¿© ´Ü¹éÁúÀÇ Æ¯¼ºÀ» È®ÀÎÇÒ ¼ö ÀÖÀ¸¸ç, »ì¾ÆÀÖ´Â ¼¼Æ÷³ª Á¶Á÷ µî¿¡¼­µµ È°¿ëÇÒ ¼ö ÀÖ´Ù. ¸ñÀû À¯ÀüÀÚ¿Í ÇÔ²² tag¸¦ cloningÇÔÀ¸·Î½á ´Ü¹éÁú°ú ÇÔ²² ¹ßÇöµÇ¾î, À̸¦ ÀÌ¿ëÇØ ¸ñÀû ´Ü¹éÁúÀ» °ËÃâÇϰųª ÃßÃâÇÒ ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ ´Ü¹éÁú tag´Â his- (polyhistidine), FLAG- (DYKDDDDK), GST-, Myc-tags µî ´Ù¾çÇÑ Á¾·ù·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.

[Application]
´ëºÎºÐÀÇ ´Ü¹éÁú tag´Â ´Ü¹éÁú Á¤Á¦»Ó ¾Æ´Ï¶ó, Western blotting, ELISAs, immunohistochemistry (IHC), immunocytochemistry (ICC), Çü±¤ °­µµ ÃøÁ¤, ChIP-seq µî°ú °°Àº ±â¼úÀ» ÀÌ¿ëÇÑ ´Ü¹éÁú °ËÃâ¿¡µµ »ç¿ëµÉ ¼ö ÀÖ´Ù. ƯÁ¤ tag´Â ¼÷ÁÖ ¼¼Æ÷¿¡ Ä¡¸íÀûÀÎ ´Ü¹éÁú ¹ßÇöÀ̳ª, ¹ßÇöµÈ ´Ü¹éÁúÀÌ ºÐÇصǴ °ÍÀ» ¹æÁöÇÏ´Â µîÀÇ ±â´ÉÀ» °¡Á®, ±âÁ¸ÀÇ ´Ü¹éÁú ¹ßÇö¿¡¼­ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¹®Á¦¸¦ ÇØ°áÇϴµ¥ À¯¿ëÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù (Li 2011). ¶ÇÇÑ, ÀϺΠtag´Â ¸ñÀû ´Ü¹éÁúÀ» ¼¼Æ÷ ³» ƯÁ¤ À§Ä¡¿¡ Á¸ÀçÇÏ°Ô Çϰųª, GST tag ¿Í °°ÀÌ ´Ü¹éÁú °¡¿ë¼º¸¦ Áõ°¡½ÃÅ°´Âµ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

´Ü¹éÁú tag´Â ÁÖ·Î ´Ü¹éÁúÀ» ƯÀÌÀûÀ¸·Î °ËÃâÇÏ´Â Ç×ü°¡ ¾øÀ» ¶§, Á¤Á¦¸¦ À§ÇØ »ç¿ëÇÑ´Ù. ´Ü¹éÁú ¹ßÇö º¤ÅÍ ½Ã½ºÅÛÀ» ÀÌ¿ëÇÏ¿© his (polyhistidine), FLAG (DYKDDDDK), GST, Myc¿Í °°Àº tag¸¦ ¸ñÀû ´Ü¹éÁú°ú ÇÔ²² ¹ßÇö½ÃÅ°°í, tag¸¦ ƯÀÌÀûÀ¸·Î °ËÃâÇÒ ¼ö ÀÖ´Â affinity resin ȤÀº antibody-linked beads µîÀ» ÀÌ¿ëÇØ ¸ñÀû ´Ü¹éÁúÀ» Á¤Á¦ÇÑ´Ù.

[´Ù¾çÇÑ Á¾·ùÀÇ tag]


His tag - The most versatile tag for protein purification

Myc tag - A small, immunoreactive tag

HA tag - unlikely to interfere with the function of your protein

GST tag - Binds with high affinity and specificity

FLAG tag - A common, well-characterized hydrophilic tag