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Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > Overview > ´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó¾÷Àû ÀÌ¿ë ¾È³»

´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó¾÷Àû ÀÌ¿ë ¾È³»

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»ó¾÷Àû È°¿ëÀÌ °¡´ÉÇÑ Ç°¸ñ ¸®½ºÆ®
1. ÀçÁ¶ÇÕ ´Ü¹éÁúÀÇ foldingÀ» µ½´Â chaperone plasmid set

Code

Á¦Ç°¸í

¿ë·®

º¹¼öÀÇ chaperone ºÐÀÚ°¡ È¿À²ÀûÀ¸·Î ¹ßÇöµÇ´Â plasmid set

3340P

Chaperon Plasmid Set

1 Kit


2. ÀÏ¹Ý E. coli ¹ßÇö½Ã½ºÅÛº¸´Ù »ý»êÈ¿À² ¹× °¡¿ë ¹ßÇöÀÌ Çâ»óµÈ vector system

Code

Á¦Ç°¸í

¿ë·®

Background ¹ßÇö ¾ïÁ¦, cold shock promoter·Î ¹ßÇöÈ¿À² up

3360P

pCold Vector Set

1 Set (°¢ 5 §¶)

3361P

pCold I DNA

25 §¶

3362P

pCold II DNA

25 §¶

3363P

pCold III DNA

25 §¶

3364P

pCold IV DNA

25 §¶

3365P

pCold TF DNA

25 §¶

3371P

pCold ProS2 DNA

25 §¶

3372P

pCold GST DNA

25 §¶


3. In-Fusion PCR cloningÀ¸·Î ¼Õ½±°Ô ÃÖÀûÀÇ secretion peptide screening ÈÄ ¸ñÀû´Ü¹éÁú ´ë·® ¹ßÇö

Code

Á¦Ç°¸í

¿ë·®

¸ñÀû´Ü¹éÁú¿¡ ¸Â´Â signal peptide ¼±Åà ÈÄ ´ë·®¹ßÇö

3380P

B. subtilis Secretory Protein Expression System

10 ȸ

Á¦Ç° ±¸¼º

1.

°¢ ÄÚµåÀÇ Á¤Á¦Ç°

1 set

2.

»ó¾÷Àû ÀÌ¿ë¿¡ ´ëÇÑ permission letter

1ºÎ



[Âü°í - Bacterial expression Overview ; ¿ø¹®¸µÅ©]

Selection guide for bacterial expression system

 

 ÀåÁ¡

¹è¾ç º¼·ý´ç
´Ü¹éÁú ¼öÀ²

 Àû¿ë

Low-temperature expression system

pCold DNA cold shock
expression system

· ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö ¼öÀ² ¹× ¼øµµ¸¦ ³ôÀ̴ Àú¿Â ¹ßÇö ½Ã½ºÅÛ
- Àú¿Â¿¡¼­ ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö¸¸ À¯µµÇÏ°í ±× ¿Ü¿¡ E. coli ¼÷ÁÖ ´Ü¹éÁúÀÇ ¹ßÇö ¹× protease È°¼ºÀ» ¾ïÁ¦

· Chaperone plasmid¸¦ ÇÔ²² »ç¿ëÇÏ¸é ¸ñÀû ´Ü¹éÁúÀÇ °¡¿ë¼ºÀ» ³ô¿©, ȸ¼öÀ²À» °³¼±ÇÒ ¼ö ÀÖÀ½

 10 §·
~ 90 §·/L

· ºÒ¿ë¼º ´Ü¹éÁúÀÇ ¹ßÇö

· ´Ù¾çÇÑ Á¾·ùÀÇ His-tagged protein expression vector ¶óÀξ÷

High-yield expression systems

B. subtilis Secretory Protein Expression System

· ¸ñÀû´Ü¹éÁúÀ» ¼÷ÁÖ ¹Ù±ùÀ¸·Î ºÐºñ ¹ßÇö½ÃÅ°´Â ½Ã½ºÅÛ (Secretion)À¸·Î ´Ü¹éÁú ´ë·® »ý»ê¿¡ ÀûÇÕ

· 173Á¾·ùÀÇ B. subtilis À¯·¡ secretory signal peptide¸¦ In-Fusion Cloning ½Ã½ºÅÛÀ¸·Î Æí¸® ÇÏ°Ô cloningÇÏ¿© Àû¿ë °¡´É

25 g/L ÀÌ»ó

· ¸ñÀû ´Ü¹éÁúÀÇ ´ë·® ºÐºñ (secretion) ¹è¾ç
(Large scale fermentation µî)