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Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > Overview > ´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó¾÷Àû ÀÌ¿ë ¾È³»

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[Âü°í - Bacterial expression Overview ; ¿ø¹®¸µÅ©]

Selection guide for bacterial expression system

 

 ÀåÁ¡

¹è¾ç º¼·ý´ç
´Ü¹éÁú ¼öÀ²

 Àû¿ë

Low-temperature expression system

pCold DNA cold shock
expression system

· ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö ¼öÀ² ¹× ¼øµµ¸¦ ³ôÀ̴ Àú¿Â ¹ßÇö ½Ã½ºÅÛ
- Àú¿Â¿¡¼­ ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö¸¸ À¯µµÇÏ°í ±× ¿Ü¿¡ E. coli ¼÷ÁÖ ´Ü¹éÁúÀÇ ¹ßÇö ¹× protease È°¼ºÀ» ¾ïÁ¦

· Chaperone plasmid¸¦ ÇÔ²² »ç¿ëÇÏ¸é ¸ñÀû ´Ü¹éÁúÀÇ °¡¿ë¼ºÀ» ³ô¿©, ȸ¼öÀ²À» °³¼±ÇÒ ¼ö ÀÖÀ½

 10 §·
~ 90 §·/L

· ºÒ¿ë¼º ´Ü¹éÁúÀÇ ¹ßÇö

· ´Ù¾çÇÑ Á¾·ùÀÇ His-tagged protein expression vector ¶óÀξ÷

High-yield expression systems

B. subtilis Secretory Protein Expression System

· ¸ñÀû´Ü¹éÁúÀ» ¼÷ÁÖ ¹Ù±ùÀ¸·Î ºÐºñ ¹ßÇö½ÃÅ°´Â ½Ã½ºÅÛ (Secretion)À¸·Î ´Ü¹éÁú ´ë·® »ý»ê¿¡ ÀûÇÕ

· 173Á¾·ùÀÇ B. subtilis À¯·¡ secretory signal peptide¸¦ In-Fusion Cloning ½Ã½ºÅÛÀ¸·Î Æí¸® ÇÏ°Ô cloningÇÏ¿© Àû¿ë °¡´É

25 g/L ÀÌ»ó

· ¸ñÀû ´Ü¹éÁúÀÇ ´ë·® ºÐºñ (secretion) ¹è¾ç
(Large scale fermentation µî)