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Anti-TF(Trigger Factor)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
M201
Anti-TF, Monoclonal
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.1 mg
432,800¿ø 
541,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
M201_DS.v1902Da.pdf

Á¦Ç° ¼³¸í
Trigger FactorÀÇ ºÎºÐ ÆéŸÀ̵å(165~178)-KLH ±³Â÷°áÇÕ¹°ÁúÀ» Ç׿øÀ¸·Î ÇÑ ¸¶¿ì½º ´ÜÀÏ Å¬·Ð Ç×ü

Clone No.

ƯÀ̼º

Subclass

TF 19-7F

pCold TF DNA¸¦ ÀÌ¿ëÇØ ¹ßÇö½ÃŲ TF(Trigger Factor) tagged À¶ÇÕ ´Ü¹éÁúÀÇ TF¿Í ƯÀÌÀûÀ¸·Î ¹ÝÀÀÇÑ´Ù.

¸¶¿ì½º IgG1

³»¿ë
´ÜÀÏ Å¬·Ð Ç×ü(µ¿°á°ÇÁ¶) 0.1 mg
¼ö¼Û
½Ç¿Â ÀÌÇÏ
º¸Á¸
4¡É
º» Á¦Ç°Àº ¹æºÎÁ¦¸¦ Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê½À´Ï´Ù. ³ìÀÎ ÈÄ stock ¿ë¾×(2.0 mg/ml)Àº ÇÊ¿ä¿¡ µû¶ó ºÐ ÁÖÇÏ¿©, 20¡É º¸Á¸½Ã 1³â, ȤÀº ¹æºÎÁ¦(0.1% Sodium Azide)¸¦ ÷°¡ÇÏ¿© 4¡É¿¡¼­ ¾à 6°³¿ù°£ º¸Á¸°¡´ÉÇÕ´Ï´Ù. µ¿°á À¶ÇØ ¹Ýº¹Àº È¿À²À» ÀúÇϽÃų ¼ö ÀÖÀ¸¸ç, ¶ÇÇÑ Èñ¼® ÈÄÀÇ º¸Á¸Àº °¡´ÉÇÑ ÇÑ ÇÇÇØ ÁÖ½Ç °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
À¯·¡
Trigger FactorÀÇ ºÎºÐ ÆéƼµå(165~178)[TIDFTGSVDGEEFE]-KLH ±³Â÷°áÇÕ¹°ÁúÀ» °¨ÀÛ(sensitization)ÇÑ C57BL/6 ¸¶¿ì½º ¸²Æı¸¿Í ¸¶¿ì½º °ñ¼öÁ¾¼¼Æ÷ P3U1¸¦ À¶ÇÕÇØ ¾òÀº ÀâÁ¾¼¼Æ÷(hybrid cell)¸¦ SCID ¸¶¿ì½º º¹°­³»¿¡¼­ Áõ½Ä ½ÃÄÑ ¾òÀº º¹¼ö.
Á¤Á¦
Column chromatography¸¦ ÀÌ¿ëÇØ immunoglobulin(IgG) Á¤Á¦ ÈÄ, 1.0% BSA¸¦ Æ÷ÇÔÇÑ 10 mM PBS(pH7.4)¿¡ ¿ëÇØÇØ µ¿°á°ÇÁ¶.
Ç×ü ¿ëÇØ
D.W. 50 ¥ìl¿¡ ¿ëÇØ. (2.0 mg/ml, ¹æºÎÁ¦¸¦ ÷°¡ÇÏÁö ¾Ê´Â´Ù).
Èñ¼®¾×
10 mM PBS(pH7.4)
1.0 % BSA
(0.1% Sodium Azide)
±³Â÷ ¹ÝÀÀ
´ëÀå±Õ À¯·¡ÀÇ ´Ù¸¥ ´Ü¹éÁú¿¡´Â ¹ÝÀÀÇÏÁö ¾Ê´Â´Ù.
ProS2 tag¿¡ ¹ÝÀÀÇÏÁö ¾Ê´Â´Ù.
¿ëµµ
* pCold TF DNA¸¦ ÀÌ¿ëÇØ ¹ßÇö½ÃŲ TF-tagged À¶ÇÕ ´Ü¹éÁú °ËÃâ
- Western Blotting (1~5 ¥ìg/ml) : ȯ¿ø ¹× ºñȯ¿ø Á¶°Ç
- ¸é¿ª ħ°­(5~10 ¥ìg/ml)

Keyword :

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
>
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
>
ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
>
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
>
Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
>
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
>
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
>
¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)