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50 mM DTT, 5 mM EDTA¸¦ ÇÔÀ¯ÇÏ´Â 250 mM Sodium phosphate ¹öÆÛ (pH7.0) 1 ml
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ÃÊ°í¿Â¼º ¼¼±ÕÀÎ Pyrococcus furiosus À¯·¡ÀÇ Ãʳ»¿¼º È¿¼Ò·Î N-¸»´ÜÀÌ pyroglutamate·Î blockingµÈ ÆéŸÀÌµå ¹× ´Ü¹éÁú¿¡¼ °í¿ÂÀÇ ´Ü¹éÁú º¯¼ºÁ¶°Ç¿¡¼ ³ôÀº È¿À²·Î pyroglutamate¸¦ À¯¸®ÇÑ´Ù.
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Pyrococcus furiosus ; À¯ÀüÀÚ ÀçÁ¶ÇÕ ±â¼ú·Î ´ëÀå±Õ¿¡¼ »ý»ê
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N-¸»´ÜÀÌ pyroglutamateÀÎ ÆéŸÀÌµå ¹× ´Ü¹éÁú·ÎºÎÅÍ pyroglutamate¸¦ À¯¸®ÇÑ´Ù.
È°¼ºÀÇ Á¤ÀÇ
Pyroglutamate-p-nitroanilide¸¦ ±âÁú·Î ÇÏ¿©, pH7.0, 37¡É¿¡¼ 1ºÐ µ¿¾È 1 ¥ìmolÀÇ p-nitroanilineÀ» »ý¼ºÇÏ´Â È¿¼ÒÈ°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
Specific Activity
15.4 U/ mg protein (37¡É¿¡¼ ÃøÁ¤, ´ëÇ¥½Ã·á ºÐ¼®Ä¡)
100 U/ mg protein (75¡É¿¡¼ ÃøÁ¤, ´ëÇ¥½Ã·á ºÐ¼®Ä¡)
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SDS-PAGE¿¡¼ ¼øµµ 90% ÀÌ»ó, ±âŸ proteaseÀÇ È°¼ºÀº ³ªÅ¸³ªÁö ¾Ê´Â´Ù.
License Notice : [L10, M33]