´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Vector

pCold¢â TF DNA

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3365
pCold¢â TF DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
1,011,200¿ø 
1,264,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3365_e.v1909Da.pdf



*º» Á¦Ç°±ºÀ» »ó¾÷Àû ¿ëµµ·Î ±¸¸ÅÇÏ´Â °æ¿ì ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
Á¦Ç°¼³¸í
pCold¢â TF DNA´Â E. coli ChaperoneÀÇ ÇϳªÀÎ Trigger Factor (TF, ºÐÀÚ·® 48kDa)¸¦ soluble tag·Î ÇÏ´Â fusion Cold Shock expression vectorÀÌ´Ù. pCold DNA¿Í ¸¶Âù°¡Áö·Î E. coli Cold Shock À¯ÀüÀÚÀÇ ÇϳªÀÎ cspA À¯ÀüÀÚÀÇ promoter¸¦ ÀÌ¿ëÇÏ°í ÀÖÀ¸¸ç, cspA promoterÀÇ down-stream¿¡ 5¡¯ untranslated region (5'UTR), translation enhancing element (TEE), His tag, TF tag, multi-cloning site (MCS) µîÀÌ ¹èÄ¡µÇ¾î ÀÖ°í, Promoter downstream¿¡´Â ¹ßÇöÀ» Á¦¾îÇϱâ À§ÇÑ lac operator°¡ »ðÀԵǾî ÀÖ´Ù.
TF tag¿Í MCS »çÀÌ¿¡´Â HRV 3C Protease, Thrombin, Factor Xa Àνļ­¿­ÀÌ ÀÖ¾î ¹ßÇö ÈÄ À¶ÇÕ ´Ü¹éÁú¿¡¼­ tag¸¦ Á¦°ÅÇÏ´Â µ¥¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Cold Shock expression systemÀº Àú¿Â¿¡¼­ ¸ñÀû´Ü¹éÁúÀÇ ¹ßÇöÀ» À¯µµÇϱ⠶§¹®¿¡ ¼÷ÁÖ À¯·¡ ´Ü¹éÁúÀÇ ÇÕ¼ºÀº ¾ïÁ¦µÇ°í ¸ñÀû ´Ü¹éÁú¸¸À» °íÈ¿À²·Î ¾òÀ» ¼ö ÀÖ¾î ±âÁ¸ÀÇ E. coli ¹ßÇö½Ã½ºÅÛ°ú ºñ±³ÇÏ¿© ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö·®À̳ª °¡¿ë¼ºÀÇ Çâ»óÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ TF soluble tag°ú chaperone ±â´ÉÀ¸·Î ÀÎÇØ ±× µ¿¾È ¹ßÇöÀÌ ¾î·Á¿ü´ø À¯ÀüÀÚ¸¦ º¸´Ù ³ôÀº È®·ü·Î soluble proteinÀ¸·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù.
*Translation enhancing elementÀÇ ¾àÀÚ·Î, ¹ø¿ªÀ» ÃËÁøÇÏ´Â ±â´ÉÀ» °¡Áø´Ù.


±×¸². pCold¢â TF vector map
º¸Á¸
-20 ¡É
GenBank
Accession No. : AB213654
»ç¿ë »ó ÁÖÀÇ»çÇ×
º» Á¦Ç°Àº ¿¬±¸ ¸ñÀûÀ¸·Î¸¸ »ç¿ë °¡´ÉÇÑ Á¦Ç°ÀÔ´Ï´Ù (Research use only).
º» Á¦Ç° ¶Ç´Â º» Á¦Ç°À¸·Î ¸¸µé¾îÁø »ê¹°À» »ó¾÷Àû ¸ñÀûÀ¸·Î ÀÌ¿ëÇÏ´Â °æ¿ì, ¶óÀ̼±½º°¡ Æ÷ÇÔµÈ Á¦Ç°À» ±¸ÀÔÇÏ½Ç ¼ö ÀÖÀ¸´Ï ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
°ü·ÃÁ¦Ç°
- ÀçÁ¶ÇÕ ´Ü¹éÁú ¹ßÇö¿ë E. coli Comp. cell : TaKaRa Competent Cells BL21
- Â÷¼¼´ë Membrane ±â¼úÀÇ His-tagged protein Á¤Á¦ column : Capturem¢â His-Tagged Purification
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî

Keyword :

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
>
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
>
ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
>
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
>
Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
>
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
>
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
>
¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)