Mammalian expression Overview
Mammalian expression system |
|
Applications/advantages |
CHOgro¢ç High Yield
Expression System |
- Suspension CHO cellÀ» ÀÌ¿ëÇÑ Ç×ü/´Ü¹éÁú ¹ßÇö¿ë transfection & medium system
- Chemically defined, hydrolysate-free, animal origin-free medium Æ÷ÇÔ
- Ç×ü, ´Ü¹éÁúÀ» °í¼öÀ²·Î ¹ßÇö
- Dry power, polybag µî ´Ù¾çÇÑ ÇüÅÂÀÇ medium º°µµ ÆǸÅ
|
Human Cell-Free
Protein
Expression System |
- Á÷Á¢ ¼¼Æ÷¸¦ ¹è¾çÇÏÁö ¾Ê°í, Human cell extract¸¦ ÀÌ¿ëÇÑ in vitro ´Ü¹éÁú ÇÕ¼º ½Ã½ºÅÛ
- ÇϳªÀÇ Æ©ºê¿¡¼ RNA Àü»çü·ÎºÎÅÍ ´Ü¹éÁú ÇÕ¼º±îÁö ¿Ï·á
- 150 kDaÀÌ»óÀÇ °íºÐÀÚ ´Ü¹éÁú ÇÕ¼º¿¡µµ ÀÀ¿ë °¡´É
- His-Tag³ª c-Myc-tagged protein expression vector º°µµ ÆǸÅ
|
pHEK293 Ultra
Expression Vectors |
- Human HEK293 ¼¼Æ÷¿¡¼ transient overexpression¿¡ ÃÖÀûÈµÈ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ
- TAR-Tat systemÀ» ÀÌ¿ëÇÏ¿©, ±âÁ¸ÀÇ CMV À¯·¡ ÇÁ·Î¸ðÅÍ ½Ã½ºÅÛº¸´Ù ÃÖ´ë 10¹è ³ôÀº
´Ü¹éÁú ¹ßÇö °¡´É
|
HA-tag & Myc-tag
Expression System |
- 10 §¶/§¢ - 10 §¶/§¢
- N- / C-¸»´ÜÀÇ HA-tagged ¶Ç´Â Myc-tagged expression vector Æ÷ÇÔ
- HA-tagging ´Ü¹éÁú ¶Ç´Â Myc-tagging ´Ü¹éÁúÀ» ¹ßÇö½ÃÄÑ Western blot, ELISA,
immunoprecipitation, immunostaining µî¿¡ Àû¿ë °¡´É
|