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Gene transfer (virus)
>
Mammalian Cell ¿ë Vector
> epitope tag (HA or c-Myc) Vectors
epitope tag (HA or c-Myc) Vectors
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Clontech
631206
c-Myc Monoclonal Antibody
200 ¥ìg
779,000¿ø
Clontech
631208
c-Myc Monoclonal Antibody-Agarose Beads
1 ml
1,186,000¿ø
Clontech
635689
pCMV-Myc Vector Set
each
1,020,000¿ø
,
,
Clontech
635690
pCMV-HA Vector Set
each
1,020,000¿ø
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Myc-Tag & HA-Tag Vectors
Æ÷À¯·ù ¼¼Æ÷¿¡¼ myc-tagged ¶Ç´Â HA-tagged proteinsÀÇ Co-immunoprecipitation¿¡ Àû¿ë
Áö¼ÓÀûÀÌ°í ³ôÀº ´Ü¹éÁú ¹ßÇö
Æ÷À¯·ù ¼¼Æ÷¿¡¼ yeast two-hybrid interactions È®Àο¡ Àû¿ë
Western blotÀ» ÅëÇØ ¼±¸íÇÑ band È®ÀÎ °¡´É
c-MycÀ̳ª hemagglutinin (HA) epitope tagÀ¸·Î Ç¥½ÄµÈ ´Ü¹éÁú Æ÷À¯·ù ¹ßÇö º¤ÅÍÀÌ´Ù. c-Myc¿Í HA´Â ¸é¿ª¹ÝÀÀ¿ëÀ¸·Î ƯȵǾî ÀÖ¾î co-immunoprecipitation ¿¬±¸ µî¿¡ ÀÌ»óÀûÀ̸ç western blotÀ¸·Î ½±°Ô °ËÃâ ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ c-Myc¿Í HA´Â ÀÛÀº tag·Î ¸ñÀû À¯ÀüÀÚÀÇ ±â´É¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â´Ù.
pCMV vector
´Â CMV promoter¸¦ ÀÌ¿ëÇØ ´Ù¾çÇÑ Æ÷À¯·ù ¼¼Æ÷¿¡¼ Áö¼ÓÀûÀÌ°í ³ôÀº ¹ßÇöÀ» º¸ÀδÙ. MycÀ¸·Î Ç¥ÁöµÈ ¸ñÀû ´Ü¹éÁúÀº western blotÀ̳ª anti-myc antibody¸¦ »ç¿ëÇÏ¿© fluorescence microscopy·Î °ËÃâÇÏ´Â ¹æ¹ýÀÌ ÀÖ´Ù.
pCMV-Myc & pCMV-HA Vector Set
´Â c-Myc Monoclonal Antibody¿Í HA-Tag Polyclonal Antibody¸¦ Æ÷ÇÔÇÏ°í ÀÖÀ¸¸ç ÀÌ Ç×ü¸¦ ÀÌ¿ëÇϸé Western blot, ELISA, ¶Ç´Â ¸é¿ª¿°»ö¹ý immunostaining assays¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ insertÀÇ reading frameÀÌ ¿Ã¹Ù¸¥Áö ¿©ºÎ¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Â sequencing primer°¡ Æ÷ÇԵǾî ÀÖ´Ù.
HA Tag sequence
: ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT
Myc Tag sequence
: ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG
pCMV-Myc and pCMV-HA vector maps.
Keyword :
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
epitope tag (HA or c-Myc) Vectors
in vivo Vector, pLIVE Vector ½Ã¸®Áî
Magnetic Myc IP Kit
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-CMVvector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
pIRES Bicistronic Expression Vector