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Gene transfer (virus)
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Lentivirus
> Lentivirus Á¤Á¦
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀûÀÎ Lentivirus Á¤Á¦
Lentivirus Á¤Á¦
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Clontech
631233
Lenti-X¢â Maxi Purification Kit
2 ȸ
570,000¿ø
Clontech
631234
Lenti-X¢â Maxi Purification Kit
5 ȸ
924,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
ÇüÁúµµÀÔ ÀúÇØÁ¦¿Í ¼¼Æ÷µ¶¼º ÀÖ´Â ¿À¿°¹°ÁúÀÇ È¿°úÀûÀÎ Á¦°Å
¹ÙÀÌ·¯½º¸¦ 10 ¹è±îÁö ³óÃà
°£ÆíÇÑ gravity-flow Á¶ÀÛ °úÁ¤
È¿À²ÀûÀÌ°í ÀÏ°ü¼º ÀÖ´Â ÇüÁúµµÀÔ°ú ´õ¿í °Ç°ÇÑ ¼¼Æ÷¸¦ À§ÇØ »ç¿ë
Lenti-X Maxi Purification Kit´Â Á¤Á¦¸¦ °ÅÄ¡Áö ¾ÊÀº crude »óÃþ¾×À¸·Î ºÎÅÍ ¸Å¿ì ¸¹Àº ¾çÀÇ ¹ÙÀÌ·¯½º¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Filter¸¦ ÀÌ¿ëÇÑ Á¤Á¦ ¹æ¹ýÀº lentivirus ÀÔÀÚ¿¡ ¼Õ»óÀ» Áְųª Á¤Á¦·®À» °¨¼Ò½ÃÅ°´Âµ¥ ºñÇÏ¿© gravity column ¹æ½ÄÀÇ ½ÇÇèÁ¶ÀÛÀ¸·Î ºü¸£°í °£ÆíÇϸç È¿°úÀûÀÌ´Ù. Gravity-flow columnÀº ¼¼Ã´ °úÁ¤ µ¿¾È columnÀ» ÅëÇØ ºÎÂøµÇÁö ¾ÊÀº ¹°ÁúµéÀÌ Èê·¯°¡´Â ¹Ý¸é »óÃþ¾× ³»¿¡ ¹ÙÀÌ·¯½º ÀÔÀÚµéÀÌ ³²¾ÆÀÖ°Ô µÇ¾î, Á¤Á¦µÈ ¹ÙÀÌ·¯½º´Â ¿ÂÀüÇÑ ÇüÅÂ¿Í ±â´ÉÀ» ȸº¹ÇÑ´Ù. º» ½Ã½ºÅÛÀº ¹ÙÀÌ·¯½º¸¦ Á¤Á¦ÇÏ´Â °úÁ¤ÀÌ plasmid DNA Á¤Á¦¸¸Å °£ÆíÇÏ´Ù.
¿Ö Lentivirus¸¦ Á¤Á¦ÇØ¾ß Çϴ°¡?
¹ÙÀÌ·¯½ºÀÇ Á¤Á¦´Â ÇüÁú µµÀÔÇÏ´Â ½ÇÇè¿¡ ¿µÇâÀ» ¹ÌÄ¥ ¼ö ÀÖ´Â ¼¼Æ÷ ¿À¿°¹°Áúµµ Á¦°ÅÇÒ ¼ö ÀÖ´Ù. Á¤Á¦µÇÁö ¾ÊÀº »óÃþ¾×Àº ÀÜÁ¸ plasmid DNA, ¾Ë·ÁÁöÁö ¾ÊÀº °¨¿°°ú ÇüÁú µµÀÔ ÀúÇØÁ¦, ¼¼Æ÷ ³» ´Ü¹éÁú°ú Ç÷û ´Ü¹éÁú, °íµµÀÇ ¸é¿ª¼º ¹ÙÀÌ·¯½º ´Ü¹éÁú, ÇÙ»ê, ¹ÙÀÌ·¯½º ´ÜÆíÀ» Æ÷ÇÔÇÏ°í ÀÖ´Ù. ¹Î°¨ÇÑ ¸ñÀû ¼¼Æ÷ ¶Ç´Â in vivo Àû¿ëÀ» À§Çؼ´Â lentivirus »ç¿ë Àü¿¡ ¹ÙÀÌ·¯½º¸¦ Á¤Á¦ÇÏ¿© ÀÌ·¯ÇÑ ¿À¿°¹°ÁúÀ» Á¦°ÅÇÏ´Â °ÍÀÌ ÇʼöÀûÀÌ´Ù.
Á¤Á¦µÈ ¹ÙÀÌ·¯½º¸¦ »ç¿ëÇÏ¸é ¡®pseudo-transduction¡® ¶ÇÇÑ ¹æÁöÇÒ ¼ö ÀÖ´Ù.
°£´ÜÇÑ Á¤Á¦ °úÁ¤
Lenti-X Maxi ColumnÀº »ç¿ëÇϱâ Æí¸®Çϵµ·Ï ±¸¼ºµÇ¾î ÀÖ´Ù. ½Ã·á »óÃþ¾× (9~45 ml)¿¡ 10x binding buffer¸¦ ÷°¡ÇÑ ÈÄ column¿¡ ³ÖÀ¸¸é Á߷¿¡ ÀÇÇØ ½Ã·á¿Í buffer°¡ Åë°úÇÏ°í, 2 ȸ column ¼¼Ã´ °úÁ¤ µ¿¾È ºÒ¼ø¹°µéÀº Á¦°ÅµÇ°í 3 mlÀÇ elution buffer·Î Á¤Á¦µÈ ¹ÙÀÌ·¯½º¸¦ ȸ¼öÇÑ´Ù.
Filter Systemº¸´Ù ¶Ù¾î³ ȸ¼ö·Â°ú ¼øµµ
¹ÙÀÌ·¯½ºÀÇ °¨¿°·ÂÀ» À¯ÁöÇÏ°í ¶Ù¾î³ ¹ÙÀÌ·¯½º ȸ¼ö´É·ÂÀ» ¹ßÈÖÇÏ´Â °ÍÀÌ Á¤Á¦ °úÁ¤ÀÇ ÇÙ½ÉÀÌ´Ù. ÀϹÝÀûÀ¸·Î Lenti-X columnÀº ½Ã·á¿¡¼ 60% ÀÌ»óÀÇ ¹ÙÀÌ·¯½º°¡ Á¤Á¦µÇ°í ¶ÇÇÑ °¨¿°·ÂÀÌ ÀÖ´Ù (Figure 2). Anion exchange-based membrane systemÀº Lenti-X column º¸´Ù ³·Àº ȸ¼ö´É·ÂÀ» º¸À̸ç Á¾Á¾ 20% ÀÌÇÏÀÎ °æ¿ìµµ ÀÖ´Ù. Lenti-X columnÀº filter systemº¸´Ù ´õ ³ôÀº ¹ÙÀÌ·¯½º ¼øµµ¿Í ¸Å¿ì ÀûÀº ´Ü¹éÁú °ËÃâÀ» º¸ÀδÙ. Filter Á¤Á¦ ¹æ½ÄÀº ¹ÙÀÌ·¯½º¿ÍÇÔ²² Á¤Á¦µÈ ¿ÜºÎ ´Ü¹éÁúÀ» ´Ù·® Æ÷ÇÔÇÏ°í ÀÖ´Ù.
Figure 1. The Lenti-X Maxi Purification Kit allows you to generate high yields of purified lentivirus from crude packaging cell supernatants.
The gravity column-based method (bind, wash, elute) is extremely simple and effective, and preserves virus infectivity much better than filter-based methods.
Figure 2. The Lenti-X Maxi Purification Kit produces excellent yields of highly purified lentivirus
. Virus content in the indicated column fractions was tracked using either Lenti-X qRT-PCR (RNA copies) or flow cytometry/ fluorescence (IFU) titration. The mean values from seven experiments are shown.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System