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Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packaging cell line

Packaging (293T Cell Line)

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Clontech
632273
AAVpro¢ç 293T Cell Line
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1 ml
737,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32372

[ÁÖÀÇ] µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
       ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.

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AAVpro 293T Cell lineÀº °í¿ª°¡ÀÇ Adeno-associated virus (AAV) Á¦ÀÛÀ» À§ÇÑ AAV packaging¿ë HEK293T Cell line À¯·¡ÀÇ ¼¼Æ÷ÁÖÀÌ´Ù. º» ¼¼Æ÷ÁÖ´Â SV40 Large T antigenÀ» »ó½Ã ¹ßÇöÇϸç, AAVpro Helper Free System (Code 6230)°ú ÇÔ²² »ç¿ëÇÏ¸é º¸´Ù ³ôÀº ¿ª°¡ÀÇ AAV¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù.
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AAVpro 293T Cell Line 1 ml(2.0¡¿106 cells/tube)
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¾×üÁú¼Ò(-196¡ÆC) ¶Ç´Â -150¡É freezer¿¡ º¸°ü
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Cell Freezing Medium, DMSO 1X (Sigma-Aldrich, C6164)
Shipping Conditions
Dry ice (-70¡É)
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Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)

Keyword : Adeno Associated Virus,in vivo,aav virus

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
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Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
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¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
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Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
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AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
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Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
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´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
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AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
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AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
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ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
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°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
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±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution