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Gene transfer (virus)
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Adeno-Associated Virus (AAV)
> Packaging (293T Cell Line)
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packaging cell line
Packaging (293T Cell Line)
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Clontech
632273
AAVpro¢ç 293T Cell Line
1 ml
737,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
[ÁÖÀÇ]
µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE:
We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
³ôÀº ¿ª°¡ÀÇ Adeno-associated virus (AAV) Á¦ÀÛ¿¡ ÃÖÀûÀÎ 293T Cell line
AAVpro Helper Free System (Code 6230 ¿Ü)
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AAVpro 293T Cell lineÀº °í¿ª°¡ÀÇ Adeno-associated virus (AAV) Á¦ÀÛÀ» À§ÇÑ AAV packaging¿ë HEK293T Cell line À¯·¡ÀÇ ¼¼Æ÷ÁÖÀÌ´Ù. º» ¼¼Æ÷ÁÖ´Â SV40 Large T antigenÀ» »ó½Ã ¹ßÇöÇϸç,
AAVpro Helper Free System (Code 6230)
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AAVpro 293T Cell Line 1 ml(2.0¡¿10
6
cells/tube)
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¾×üÁú¼Ò(-196¡ÆC) ¶Ç´Â -150¡É freezer¿¡ º¸°ü
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Cell Freezing Medium, DMSO 1X (Sigma-Aldrich, C6164)
Shipping Conditions
Dry ice (-70¡É)
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Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
Keyword : Adeno Associated Virus,in vivo,aav virus
Adeno-Associated Virus (AAV)
Overview - All about AAV
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
Cell°ú ¹è¾ç ¹èÁö¿¡¼ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution