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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Mammalian Cell ¿ë Vector > pIRES Bicistronic Expression Vector

pIRES Bicistronic Expression Vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631605
pIRES Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33396
Clontech
631622
pIRESbleo3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33402
Clontech
631620
pIREShyg3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33403
Clontech
631621
pIRESneo3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33404
Clontech
631619
pIRESpuro3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33405
Clontech
632435
pIRES2-AcGFP1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,161,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33398
Clontech
632515
pIRES2-AcGFP1-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,161,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33399
Clontech
632420
pIRES2-DsRed2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,161,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33400
Clontech
632540
pIRES2 DsRed-Express2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,126,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33397
Clontech
632478
pIRES2-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
1,161,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33401
Clontech
631971
pEF1¥á-IRES-AcGFP1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33355
Clontech
631980
pEF1¥á-IRES-DsRed-Express2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33356
Clontech
631976
pEF1¥á-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
1,060,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33357
Clontech
632520
pRetroX-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
1,121,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33539, x33005
Clontech
632521
pRetroX-IRES-DsRed-Express Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,135,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33538, x33005
Clontech
632187
pLVX-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
1,333,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33451, x33863
Clontech
631237
pLVX-IRES-mCherry Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ug
1,358,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33447, x33859
Clontech
631238
pLVX-IRES-tdTomato Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ug
1,358,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33450, x33862
Clontech
631982
pLVX-EF1a-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 µg
1,291,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33434, x33848
Clontech
631987
pLVX-EF1a-IRES-mCherry Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33432, x33846

  • ÇϳªÀÇ mRNA·ÎºÎÅÍ ¸ñÀû ´Ü¹éÁú°ú selection marker (Ç×»ýÁ¦, Çü±¤ ´Ü¹éÁú) µ¿½Ã ¹ßÇö
  • Selection marker¸¦ ÀÌ¿ëÇØ, ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ¼¼Æ÷ÀÇ ½Äº° °úÁ¤ ´ÜÃà
  • ´õ ºü¸£°í, ¾ÈÁ¤ÀûÀ¸·Î cloneÀ» ¼±º°Çϱâ À§ÇÑ bicistronic expression
IRESÀÇ È°¿ë
IRES (Internal Ribosome Entry Site) promoter°¡ µµÀÔµÈ vector´Â ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â cloneÀ» ºü¸£°í È¿°úÀûÀ¸·Î ¼±º°ÇÒ ¼ö ÀÖ°Ô ÇÑ´Ù. ÀÌ vector´Â ÇϳªÀÇ promoter·Î ¸ñÀû À¯ÀüÀÚ¿Í ¼±º° ¸¶Ä¿ (Figure 1) ¶Ç´Â Living Colors Fluorescent Protein (Figure 2) ¸ðµÎ¸¦ ¹ßÇöÇÒ ¼ö ÀÖ´Â cassette¸¦ °¡Áö°í ÀÖ´Ù. ±×·¯¹Ç·Î ½ÇÁúÀûÀ¸·Î ¸¶Ä¿¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷´Â ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇÑ´Ù. ÀÌ·± vector¸¦ ÀÌ¿ëÇϸé ÃÖ¼ÒÇÑÀÇ ¼±º°°úÁ¤À¸·Î ¸ñÀû ´Ü¹éÁúÀ» ³ô°Ô ¹ßÇöÇÏ´Â cloneÀ» ¼±º°ÇÒ ¼ö ÀÖ´Ù.

 
Figure 1. Map of the pIRES Selection
Vectors.
Figure 2. Map of the Fluorescent pIRES2
Bicistronic Expression Vectors
.
Ç×»ýÁ¦¸¦ Selection marker·Î ¹ßÇöÇÏ´Â pIRES2 VectorÀÇ È°¿ë
pIRESpuro3, pIREShyg3, pIRESneo3, pIRESbleo3¿Í °°ÀÌ Ç×»ýÁ¦¸¦ selection marker·Î »ç¿ëÇÏ´Â pIRES2 vector´Â ¸ñÀû À¯ÀüÀÚ¿Í selection marker¸¦ ÇϳªÀÇ mRNA·Î ¹ßÇöÇÑ´Ù. ÀÌ vector°¡ µµÀԵǾî puromycin, hygromycin, G418, bleomycin¿¡ ÀúÇ×¼º ÀÖ´Â ¼¼Æ÷ ´ëºÎºÐÀº ¸ñÀû ´Ü¹éÁúÀ» ³ô°Ô ¹ßÇöÇÑ´Ù.
Çü±¤ ´Ü¹éÁúÀ» Selection marker·Î ¹ßÇöÇÏ´Â pIRES2 VectorÀÇ È°¿ë
Living Colors pIRES2 Vector´Â ¿ÏÀüÈ÷ È°¼ºÈ­µÈ IRES ¼­¿­À» Æ÷ÇÔÇÏ°í ÀÖ¾î Çü±¤´Ü¹éÁúÀ» ³ô°Ô ¹ßÇö½Ãų ¼ö ÀÖ´Ù. ¸¶Ä¿´Â À¶ÇÕ ´Ü¹éÁúÀ̱⠺¸´Ù´Â °³º°ÀûÀÎ ´Ü¹éÁúÀ̱⠶§¹®¿¡ ¸ñÀû ´Ü¹éÁúÀÇ »ý¹°ÇÐÀû È°¼º¿¡ ´ëÇÑ À§ÇèÀÌ °ÅÀÇ ¾ø´Ù. pIRES2 vector´Â ÀϽÃÀûÀ¸·Î transfectionµÈ ¼¼Æ÷ÀÇ ³óÃàÀ̳ª Çü±¤ °­µµ¸¦ ÅëÇØ ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇö ¼öÁØÀ» °üÂû Çϴµ¥ À¯¿ëÇÏ°Ô È°¿ëµÉ ¼ö ÀÖ´Ù.


Figure 3. Schematic diagram of bicistronic mRNA translation.
The internal ribosome entry site (IRES) permits a protein of interest and either an antibiotic resistance marker or fluorescent protein to be translated from the same mRNA. IVS = synthetic intron.
±¸¼º ¹× º¸°ü
- ÀÚ¼¼ÇÑ ±¸¼º°ú º¸°ü Á¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
- Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477-483.
- Jang, S. K. et al. (1988) J. Virol. 62:2636-2643.
- Rees, S. et al. (1996) Biotechniques 20:102-110.

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