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Gene transfer (virus)
>
Mammalian Cell ¿ë Vector
> pIRES Bicistronic Expression Vector
pIRES Bicistronic Expression Vector
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
631605
pIRES Vector
20 ¥ìg
840,000¿ø
Clontech
631622
pIRESbleo3 Vector
20 ¥ìg
840,000¿ø
Clontech
631620
pIREShyg3 Vector
20 ¥ìg
840,000¿ø
Clontech
631621
pIRESneo3 Vector
20 ¥ìg
840,000¿ø
Clontech
631619
pIRESpuro3 Vector
20 ¥ìg
840,000¿ø
Clontech
632435
pIRES2-AcGFP1 Vector
20 ¥ìg
1,161,000¿ø
Clontech
632515
pIRES2-AcGFP1-Nuc Vector
20 ¥ìg
1,161,000¿ø
Clontech
632420
pIRES2-DsRed2 Vector
20 ¥ìg
1,161,000¿ø
Clontech
632540
pIRES2 DsRed-Express2 Vector
20 ¥ìg
1,126,000¿ø
Clontech
632478
pIRES2-ZsGreen1 Vector
20 ¥ìg
1,161,000¿ø
Clontech
631971
pEF1¥á-IRES-AcGFP1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631980
pEF1¥á-IRES-DsRed-Express2 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631976
pEF1¥á-IRES-ZsGreen1 Vector
10 ¥ìg
1,060,000¿ø
Clontech
632520
pRetroX-IRES-ZsGreen1 Vector
20 ¥ìg
1,121,000¿ø
,
Clontech
632521
pRetroX-IRES-DsRed-Express Vector
20 ¥ìg
1,135,000¿ø
,
Clontech
632187
pLVX-IRES-ZsGreen1 Vector
10 ¥ìg
1,333,000¿ø
,
Clontech
631237
pLVX-IRES-mCherry Vector
20 ug
1,358,000¿ø
,
Clontech
631238
pLVX-IRES-tdTomato Vector
20 ug
1,358,000¿ø
,
Clontech
631982
pLVX-EF1a-IRES-ZsGreen1 Vector
10 µg
1,291,000¿ø
,
Clontech
631987
pLVX-EF1a-IRES-mCherry Vector
10 µg
1,352,000¿ø
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
ÇϳªÀÇ mRNA·ÎºÎÅÍ ¸ñÀû ´Ü¹éÁú°ú selection marker (Ç×»ýÁ¦, Çü±¤ ´Ü¹éÁú) µ¿½Ã ¹ßÇö
Selection marker¸¦ ÀÌ¿ëÇØ, ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ¼¼Æ÷ÀÇ ½Äº° °úÁ¤ ´ÜÃà
´õ ºü¸£°í, ¾ÈÁ¤ÀûÀ¸·Î cloneÀ» ¼±º°Çϱâ À§ÇÑ bicistronic expression
IRESÀÇ È°¿ë
IRES (Internal Ribosome Entry Site) promoter°¡ µµÀÔµÈ vector´Â ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â cloneÀ» ºü¸£°í È¿°úÀûÀ¸·Î ¼±º°ÇÒ ¼ö ÀÖ°Ô ÇÑ´Ù. ÀÌ vector´Â ÇϳªÀÇ promoter·Î ¸ñÀû À¯ÀüÀÚ¿Í ¼±º° ¸¶Ä¿ (Figure 1) ¶Ç´Â Living Colors Fluorescent Protein (Figure 2) ¸ðµÎ¸¦ ¹ßÇöÇÒ ¼ö ÀÖ´Â cassette¸¦ °¡Áö°í ÀÖ´Ù. ±×·¯¹Ç·Î ½ÇÁúÀûÀ¸·Î ¸¶Ä¿¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷´Â ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇÑ´Ù. ÀÌ·± vector¸¦ ÀÌ¿ëÇϸé ÃÖ¼ÒÇÑÀÇ ¼±º°°úÁ¤À¸·Î ¸ñÀû ´Ü¹éÁúÀ» ³ô°Ô ¹ßÇöÇÏ´Â cloneÀ» ¼±º°ÇÒ ¼ö ÀÖ´Ù.
Figure 1. Map of the pIRES Selection
Vectors.
Figure 2. Map of the Fluorescent pIRES2
Bicistronic Expression Vectors
.
Ç×»ýÁ¦¸¦ Selection marker·Î ¹ßÇöÇÏ´Â pIRES2 VectorÀÇ È°¿ë
pIRESpuro3, pIREShyg3, pIRESneo3, pIRESbleo3¿Í °°ÀÌ Ç×»ýÁ¦¸¦ selection marker·Î »ç¿ëÇÏ´Â pIRES2 vector´Â ¸ñÀû À¯ÀüÀÚ¿Í selection marker¸¦ ÇϳªÀÇ mRNA·Î ¹ßÇöÇÑ´Ù. ÀÌ vector°¡ µµÀԵǾî puromycin, hygromycin, G418, bleomycin¿¡ ÀúÇ×¼º ÀÖ´Â ¼¼Æ÷ ´ëºÎºÐÀº ¸ñÀû ´Ü¹éÁúÀ» ³ô°Ô ¹ßÇöÇÑ´Ù.
Çü±¤ ´Ü¹éÁúÀ» Selection marker·Î ¹ßÇöÇÏ´Â pIRES2 VectorÀÇ È°¿ë
Living Colors pIRES2 Vector´Â ¿ÏÀüÈ÷ È°¼ºÈµÈ IRES ¼¿À» Æ÷ÇÔÇÏ°í ÀÖ¾î Çü±¤´Ü¹éÁúÀ» ³ô°Ô ¹ßÇö½Ãų ¼ö ÀÖ´Ù. ¸¶Ä¿´Â À¶ÇÕ ´Ü¹éÁúÀ̱⠺¸´Ù´Â °³º°ÀûÀÎ ´Ü¹éÁúÀ̱⠶§¹®¿¡ ¸ñÀû ´Ü¹éÁúÀÇ »ý¹°ÇÐÀû È°¼º¿¡ ´ëÇÑ À§ÇèÀÌ °ÅÀÇ ¾ø´Ù. pIRES2 vector´Â ÀϽÃÀûÀ¸·Î transfectionµÈ ¼¼Æ÷ÀÇ ³óÃàÀ̳ª Çü±¤ °µµ¸¦ ÅëÇØ ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇö ¼öÁØÀ» °üÂû Çϴµ¥ À¯¿ëÇÏ°Ô È°¿ëµÉ ¼ö ÀÖ´Ù.
Figure 3. Schematic diagram of bicistronic mRNA translation.
The internal ribosome entry site (IRES) permits a protein of interest and either an antibiotic resistance marker or fluorescent protein to be translated from the same mRNA. IVS = synthetic intron.
±¸¼º ¹× º¸°ü
- ÀÚ¼¼ÇÑ ±¸¼º°ú º¸°ü Á¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
- Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477-483.
- Jang, S. K. et al. (1988) J. Virol. 62:2636-2643.
- Rees, S. et al. (1996) Biotechniques 20:102-110.
Keyword :
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
epitope tag (HA or c-Myc) Vectors
in vivo Vector, pLIVE Vector ½Ã¸®Áî
Magnetic Myc IP Kit
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-CMVvector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
pIRES Bicistronic Expression Vector