TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Ű¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø¼¾ÅÍ 
  • ¾÷¹«¾È³»¦¢ÇмúÁö¿ø
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, ÇмúÁö¿ø
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Retrovirus vector¸¦ ÀÌ¿ëÇÏ¿© Mammalian cell·Î °íÈ¿À²ÀûÀ¸·Î À¯ÀüÀÚ µµÀÔ

RetroNectin¢ç (Recombinant Human Fibronectin Fragment)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
T100A
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
0.5 mg
(0.5 ml)
262,000¿ø  T100A_B_e.v1705.pdf
Takara
T100B
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
2.5 mg
(2.5 ml)
1,001,000¿ø 
Takara
T110A
RetroNectin¢ç Dish (RetroNectin Pre-coated Dish, 35 mm¥õ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 dishes
499,000¿ø  T110A_e.v1705.pdf
Takara
T202
RetroNectin¢ç GMP grade (Recombinant Human Fibronectin Fragment CH-296)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
2.5 mg
(2.5 ml)
2,624,000¿ø  T202_DS.v1912.pdf





¡Ø RetroNectin¢ç GMP grade (Code T202) Á¦Ç° ÁÖ¹®½Ã Àü¿ë ÁÖ¹®¼­¸¦ ÀÛ¼ºÇÏ¿© ´ÙÄ«¶óÄÚ¸®¾Æ(support@takara.co.kr)·Î º¸³»ÁÖ¼¼¿ä.
¡Ø µ¿°á°ÇÁ¶ ÇüÅÂÀÇ RetroNectin¢ç GMP grade (Code T201, Á¾¸Å)ÀÌ solution ÇüÅ (Code T202) ·Î ´ëüµÇ¾ú½À´Ï´Ù.

  • ÃÖ°íÀÇ Transduction enhancer : Lentivirus, RetrovirusÀÇ À¯ÀüÀÚ µµÀÔ È¿À² ±Ø´ëÈ­
  • Low toxicity for cells : Polybrene, Protamine ´ëºñ ³·Àº µ¶¼º
  • Enhanced T cell expansion : ±âÁ¸ T cell È®´ë¹è¾ç¹ý ´ëºñ ¾ÐµµÀûÀÎ T cell È®´ë¹è¾ç °¡´É (CAR-T cell ¿¬±¸¿¡ ÃÖÀû)
Á¦Ç°¼³¸í
RetroNectin¢ç, Recombinant Human Fibronectin CH-296Àº Human fibronectin Áß ±â´ÉÀ» °¡Áø 3°³ ´Ü¹éÁú Á¶°¢ (¨ç Cell-binding domain (C-domain, VLA-5¿Í °áÇÕ), ¨è Heparin-binding domain (H-domain, virus °áÇÕ), ¨é CS-1 (VLA-4 °áÇÕ))À¸·Î ÀÌ·ç¾îÁø ÀçÁ¶ÇÕ ´Ü¹éÁúÀÌ´Ù(±×¸² 1). Target cellÀÌ VLA-4¸¦ ¹ßÇöÇϸé CS-1 ºÎÀ§¿Í, VLA-5¸¦ ¹ßÇöÇϸé Cell-binding domain°ú °¢°¢ °áÇÕÇÏ°Ô µÈ´Ù. ÇÑÆí, Lenti/Ret¡©rovirus´Â Heparin-binding domain¿¡ °áÇÕÇÏ¿©, ¼¼Æ÷¿Í virus°¡ ¹°¸®ÀûÀ¸·Î °¡±î¿öÁ® À¯ÀüÀÚ µµÀÔ È¿À²ÀÌ ±ØÀûÀ¸·Î Áõ°¡ÇÑ´Ù.

RetroNectin¢ç GMP (Code T202)´Â GMP gradeÀÇ RetroNectin¢çÀ¸·Î GMP¿¡ °üÇÑ °¡À̵å¶óÀο¡ µû¶ó Á¦Á¶ ¹× ǰÁú °ü¸®µÇ°í ÀÖ´Ù. µû¶ó¼­ ¿¬±¸¿ëÀ¸·Î ÆÇ¸ÅµÇ´Â RetroNectin¢ç (Code T100A/B)º¸´Ù °íǰÁú·Î À¯ÀüÀÚÄ¡·á³ª ¼¼Æ÷ Á¶Á¦ µîÀÇ ÀÓ»ó ¿¬±¸¿¡ »ç¿ë °¡´ÉÇÏ´Ù.



Figure 1. Hypothesized Mechanism of Action of RetroNectin¢ç. RetroNectin Reagent is a recombinant human fibronectin fragment that contains three functional domains: the (1) cell-binding domain (C-domain), (2) the heparin-binding domain (H-domain), and the (3) CS-1 sequence. RetroNectin¢ç Reagent enhances lentiviral- and retroviral-mediated gene transduction by aiding the co-localization of target cells and viral particles. Specifically, virus particles bind RetroNectin¢ç Reagent via interaction with the H-domain, and target cells bind mainly through the interaction of cell surface integri n receptors VLA-5 and/or VLA-4 with the fibronectin C-domain and CS-1 sites, respectively. By facilitating close physical proximity, RetroNectin¢ç Reagent can enhance viral-mediated gene transfer to target cells expressing integrin receptors VLA-4 and/or VLA-5.


±×¸² 2. ´Ù¾çÇÑ Áٱ⼼Æ÷¿¡ ´ëÇÑ RetrovirusÀÇ À¯ÀüÀÚ µµÀÔ È¿À² ºñ±³. ´ëÇ¥ÀûÀÎ Transduction enhancerÀÎ Polybrene, Protamine°ú RetroNectin¢çÀ» »ç¿ëÇÑ À¯ÀüÀÚ µµÀÔ È¿À² ºñ±³ °á°ú, RetroNectin¢çÀÌ ´Ù¾çÇÑ Áٱ⼼Æ÷¿¡ ´ëÇØ °¡Àå ³ôÀº À¯ÀüÀÚ µµÀÔÈ¿À²À» ³ªÅ¸³»¾ú´Ù.


Ç¥ 1. RetroNectin¢çÀ» »ç¿ëÇÑ Hematopoietic stem cell À¯·¡ ¼¼Æ÷¿¡ ´ëÇÑ À¯ÀüÀÚ µµÀÔ È¿À². RetroNectin¢çÀ» »ç¿ëÇϸé ÀϹÝÀûÀ¸·Î hard-to-transfection cell·Î ¾Ë·ÁÁø, Hematopoietic stem cell À¯·¡ ¼¼Æ÷¿¡ ´ëÇØ¼­µµ ³ôÀº È¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÏ´Ù


±×¸² 3. RetroNectin¢çÀ» »ç¿ëÇÑ È¿À²ÀûÀÎ iPS generation. Human iPS Cell Generation All-in-One Vector (Code 3671)¸¦ RetroNectin¢ç ¹× PolybreneÀ» »ç¿ëÇÏ¿© Àΰ£ ÇǺΠ¼¶À¯¾Æ¼¼Æ÷ (NHDF-Ad)¿¡ À¯ÀüÀÚ µµÀÔ ÈÄ iPS ¼¼Æ÷ ÄÝ·Î´Ï ¼ö ÃøÁ¤ÇÑ °á°ú, RetroNectin¢çÀ» »ç¿ëÇϸé È¿À²ÀûÀÎ iPS generationÀÌ °¡´ÉÇÏ´Ù.
º¸Á¸
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
- 20¡É
RetroNectin¢ç Dish (35 mm diameter Dish) (Code T110A)
4¡É
RetroNectin¢çGMP grade (Recombinant Human Fibronectin Fragment CH-296)
   - 30¡É ÀÌÇÏ
Çü»ó
RetroNectin¢ç (Recombinant Human Fibronectin Fragment) (Code T100A)
0.5 §· (0.5 §¢)
RetroNectin¢ç (Recombinant Human Fibronectin Fragment) (Code T100B)
2.5 §· (2.5 §¢)
RetroNectin¢ç Dish (35 mm diameter Dish) (Code T110A)
10 dishes
RetroNectin¢ç GMP grade (Recombinant Human Fibronectin Fragment CH-296)
    2.5 §· (2.5 §¢)
ÁÖÀÇ »çÇ×
¹«±Õ ȯ°æ¿¡¼­ ÀÛ¾÷ÇϽʽÿÀ. ¿ëÇØ ÈÄ RetroNectin¢ç ¿ë¾×Àº ½Ç¿Â¿¡ µÎ°í 12½Ã°£ À̳»¿¡ »ç¿ëÇÏ½Ã°í ¿ëÇØµÈ RetroNectin¢ç ¿ë¾×ÀÇ Àç»ç¿ëÀº ÇÇÇϽʽÿÀ.
ºñ°í
RetroNectin¢çÀº Lenti/RetrovirusÀÇ À¯ÀüÀÚ µµÀÔ È¿À² Áõ°¡»Ó¸¸ ¾Æ´Ï¶ó, T cellÀÇ È®´ë¹è¾ç¿¡µµ Àû¿ë °¡´ÉÇÑ °ÍÀÌ È®ÀεǾú´Ù. ÀϹÝÀûÀÎ T cell È®´ë ¹è¾çÀº ¹è¾ç plate¿¡ Anti-CD3 antibody (OKT3)ÀÇ coating°ú ¼¼Æ÷¹è¾ç¾×¿¡ IL-2¸¦ ÷°¡ÇÏ¿© ½Ç½ÃÇÑ´Ù. RetroNectin¢çÀº Anti-CD3 antibody¿Í ÇÔ²² coatingÇÏ¿©, ±âÁ¸ÀÇ È®´ë ¹è¾ç¹ý ´ëºñ ¾à 1.5¹è ÀÌ»ó È®´ë ¹è¾ç °¡´ÉÇϸç, ³ôÀº Naive T cell ºñÀ²À» À¯ÁöÇϴ Ư¡À¸·Î, CAR-T cell ¿¬±¸¿¡ Gold standard·Î »ç¿ëµÇ°í ÀÖ´Ù.
License Notice : [L9, M62]

Keyword : T cell,T-cell,CAR-T,À¯ÀüÀÚ Ä¡·á,¸é¿ª¼¼Æ÷,retrovirus,lentivirus,transduction,polybrene,protamine,T201

Retrovirus
>
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
>
ÇÊ¿äÇÑ À¯ÀüÀÚ¸¦ ÇϳªÀÇ vector¿¡ žÁ¦½ÃÄÑ Hum..
iPS Cell Generation¢â All-in-One Vector
>
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
>
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
>
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
>
Virus »óû¾×¿¡¼­ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
>
Retrovirus vector¸¦ ÀÌ¿ëÇÏ¿© Mammalian cell..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤