¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
¼¼Æ÷¤ýÀ¯ÀüÀÚ Ä¡·á ¿¬±¸
>
¼¼Æ÷¹è¾ç °ü·Ã Á¦Ç°
> CycleavePCR Mycoplasma Detection Kit
Real Time PCR·Î Mycoplasma ½Å¼Ó °ËÃâ
CycleavePCR Mycoplasma Detection Kit
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
CY232
CycleavePCR Mycoplasma Detection Kit
50ȸ
315,200¿ø
394,000¿ø
09.02 ~ 10.31
Product index
Overview
RetroNectin¢ç - Viral ..
[FAQ] Retronectin
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
T cell ¿¬±¸ÀÚ¸¦ À§ÇÑ ..
[Cancer Research] Immu..
Cancer ¿¬±¸ °¡À̵å
T cell therapy ¿¬±¸
RetroNectin¢ç (Recombi..
Anti-CD3 mAb GMP grade
LymphoONE¢ç T-Cell Exp..
Cytokine GoStix¢â Plus..
GT-T551 Culture medium
¼¼Æ÷ ¹è¾ç¿ë °¡½º Åõ°ú..
Gene editing
Recombinant Cas9 ´Ü¹é..
sgRNA ÇÕ¼º°ú ½ºÅ©¸®´×
TransIT-X2¢ç Transfect..
[Upgrade] Guide-it¢â L..
TCR/BCR Profiling
Human TCR a/b Profilin..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
¼¼Æ÷¹è¾ç °ü·Ã Á¦Ç°
Cell culture ÇʼöÇ° - ..
¼¼Æ÷¹è¾ç½Ã Mycoplasma ..
MycoAlert PLUS mycopla..
MycoAlert Mycoplasma D..
MycoZap Antibiotics
MycoZap Mycoplasma Eli..
TaKaRa PCR Mycoplasma ..
CycleavePCR Mycoplasma..
Ç×»ýÁ¦ ½Ã¸®Áî I
Phosphate Buffered Sal..
TBS(Tris-Buffered Sali..
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Mycoplasma 16S rRNA À¯ÀüÀÚ¸¦ Ÿ°ÙÀ¸·Î Real Time PCR ÀåÄ¡¸¦ ÀÌ¿ëÇØ ´ëºÎºÐÀÇ Mycoplasma¸¦ ƯÀÌÀûÀ¸·Î °ËÃâÇϱâ À§ÇÑ kitÀÌ´Ù. Àû¾îµµ Mycoplasma ¼Ó ÁßÀÇ 10Á¾(
M. arginini, M. hominis, M. hyorhinis, M. orale, M. salivarium, M. fermentas, M. bovis, M. arthritidis, M. pirum, M. pneumoniae
) ¹× Acholeplasma ¼Ó ÁßÀÇ 1Á¾(A. laidlawii)¿¡ ´ëÇØ ¸Å¿ì ¹Î°¨ÇÏ°Ô °ËÃâÇÒ ¼ö ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.
º» kitÀÇ ÁõÆø ¹ÝÀÀ¿¡´Â Hot Start PCR¿ë È¿¼ÒÀÎ TaKaRa Ex Taq HS¸¦ »ç¿ëÇÏ°í ÀÖ¾î ¹ÝÀÀ¾× Á¶Á¦ ȤÀº ¹ÝÀÀ(º»°ÝÀûÀÎ cycle ¹ÝÀÀ)Àü¿¡ miss-primingÀ̳ª primer dimer¿¡¼ À¯·¡ÇÏ´Â ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù.
ÁõÆø »ê¹°ÀÇ °ËÃâ¿¡´Â cycling probe¹ýÀ» »ç¿ëÇÏ°í ÀÖ´Ù. ÀÌ ¹æ¹ýÀº RNA¿Í DNA·Î ±¸¼ºµÈ Å°¸Þ¶ó probe(chimera probe)¿Í RNase H¿ÍÀÇ Á¶ÇÕ¿¡ ÀÇÇÑ °í°¨µµ °ËÃâ ¹æ¹ýÀ¸·Î, ¸Å¿ì ƯÀ̼ºÀÌ ³ôÀº °ËÃâÀÌ °¡´ÉÇÏ´Ù. Probe´Â ÇÑÂÊ ¸»´ÜÀº Çü±¤ ¹°Áú·Î, ´Ù¸¥ ¸»´ÜÀº ±× Çü±¤ ¹°ÁúÀÌ ¹ßÇÏ´Â Çü±¤À» ¼Ò±¤ÇÏ´Â ¹°Áú(quencher)·Î Ç¥ÁöµÇ¾î ÀÖ´Ù. ÀÌ cycling probe´Â intactÇÑ »óÅ¿¡¼´Â quenching¿¡ ÀÇÇØ Çü±¤À» ¹ßÇÒ ¼ö ¾øÁö¸¸, »óº¸ÀûÀÎ ÁõÆø »ê¹°°ú ÇÏÀ̺긮µå¸¦ Çü¼ºÇÑ ÈÄ¿¡ RNase H¿¡ ÀÇÇØ RNA ºÎºÐ¿¡¼ Àý´ÜµÇ¸é, °ÇÑ Çü±¤À» ¹ßÇÏ°Ô µÈ´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î Çü±¤ °µµ¸¦ ÃøÁ¤ÇÏ¿© ÁõÆø »ê¹°·®À» ¸ð´ÏÅÍ ÇÒ ¼ö ÀÖ´Ù. º» kitÀÇ probe´Â Mycoplasma¿¡ Ư¡ÀûÀÎ ¿°±â¿¡ »óº¸ÀûÀ¸·Î °áÇÕÇÏ´Â ¿°±â ºÎºÐÀ» RNA·Î ÇÏ°í Àֱ⠶§¹®¿¡, Mycoplasma¸¸À» ƯÀÌÀûÀ¸·Î °í°¨µµ¿¡ °ËÃâÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
³»¿ë
1. 2¡¿CycleavePCR Reaction Mix
625 ¥ìl ¡¡
2. Myco. Primer/Probe Mix(5¡¿conc.)
250 ¥ìl
*1
3. RNase Free dH
2
O
1 ml
4. Myco. Positive Control(1¡¿10
6
copies/¥ìl)
20 ¥ìl
*2
5. Proteinase k
50 ¥ìl ¡¡
*1
Çü±¤ Ç¥Áö probe¸¦ Æ÷ÇÔÇÏ°í ÀÖÀ¸¹Ç·Î Â÷±¤¿¡ À¯ÀÇÇÑ´Ù.
*2
Real time PCR component (1~3)¿¡ À߸øÇØ È¥ÀÔÇÏ¸é ¿Ã¹Ù¸¥ °ËÃâ ¹ÝÀÀÀ» ½Ç½ÃÇÒ ¼ö ¾ø°Ô µÈ´Ù. º°µµÀÇ º¸°üÄÉÀ̽º¸¦ ¸¶·ÃÇÏ¿© µû·Î º¸Á¸ÇÑ´Ù.
º¸Á¸
- 20¡É
kit ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ ±â±â, ½Ã¾à
* Real Time PCR¿ë ÁõÆø ÀåÄ¡ ¹× Àü¿ë Æ©ºê
Thermal Cycler Dice Real Time System II(TP900/TP960)
Thermal Cycler Dice Real Time System Lite(TP700/TP760)
Çؼ®¿¡´Â Thermal Cycler Dice Real Time System Software Ver.4.0À» »ç¿ë.
Applied Biosystems 7500 Fast Real-Time PCR System(Life Technologies)
* 200 ¥ìl, 20 ¥ìl, 10 ¥ìl °¢ micropipette, tips
* Ź»ó¿ø½É±â µî
Positive Control¿¡ ´ëÇØ
Mycoplasma Positive ControlÀ» ÁÖÇüÀ¸·Î Mycoplasma Primer·Î ÁõÆøµÈ PCR »ê¹°Àº, FAM Ç¥Áö probe °ËÃâ ¿µ¿ª°ú ROX Ç¥Áö probe °ËÃâ ¿µ¿ªÀÇ ¾çÂÊ ¸ðµÎ¸¦ Æ÷ÇÔÇÏ°í, FAM Ç¥Áö probe¿Í ROX Ç¥Áö probe ¸ðµÎ¿¡¼ °ËÃâµÈ´Ù. »ùÇà Áß¿¡ ÀúÇØ ¹°ÁúÀÌ Æ÷ÇԵǾî ÀÖ´ÂÁö È®ÀÎÇϱâ À§Çؼ´Â »ç¿ëÇÏ´Â »ùÇÿ¡ Mycoplasma Positive ControlÀ» ÷°¡ÇÏ°í Control ½ÇÇèÀ» ½Ç½ÃÇØ ROX ÇÊÅÍ·Î °ËÃâ À¯¹«¸¦ È®ÀÎÇÑ´Ù.
Keyword :
¼¼Æ÷¹è¾ç °ü·Ã Á¦Ç°
Cell culture ÇʼöÇ° - antibiotics & mycoplasma °ü·ÃÁ¦Ç°
¼¼Æ÷¹è¾ç½Ã Mycoplasma ¿À¿°, ²À È®ÀÎÇϼ¼¿ä!
MycoAlert PLUS mycoplasma detection kit
20 ºÐ À̳»¿¡ MycoplasmaÀÇ ¿À¿° °ËÃâ °¡´É
MycoAlert Mycoplasma Detection Kit
Mycoplasma ¿À¿° ¹æÁö
MycoZap Antibiotics
Mycoplasma Á¦°Å ½Ã¾à
MycoZap Mycoplasma Elimination Reagent
Nested PCR·Î Mycoplasma Á¤¹Ð°ËÃâ
TaKaRa PCR Mycoplasma Detection Set
Real Time PCR·Î Mycoplasma ½Å¼Ó °ËÃâ
CycleavePCR Mycoplasma Detection Kit
Æ÷À¯·ù ¼¼Æ÷ selection¿ë
Ç×»ýÁ¦ ½Ã¸®Áî I
Phosphate Buffered Saline (PBS) Tablets ´ë¿ë·® Á¦Ç°
TBS(Tris-Buffered Saline) tablets ´ë¿ë·® Á¦Ç°