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Home > ÀüÁ¦Ç°º¸±â > ¼¼Æ÷¤ýÀ¯ÀüÀÚ Ä¡·á ¿¬±¸ > T cell therapy ¿¬±¸ > RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Retrovirus ¹× LentivirusÀÇ transduction enhancer

RetroNectin¢ç (Recombinant Human Fibronectin Fragment)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
T100A
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
0.5 mg
(0.5 ml)
224,000¿ø 
280,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
T100A_B_e.v1705.pdf
Takara
T100B
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
2.5 mg
(2.5 ml)
857,600¿ø 
1,072,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
T110A
RetroNectin¢ç Dish (RetroNectin Pre-coated Dish, 35 mm¥õ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 dishes
426,400¿ø 
533,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
T110A_e.v1705.pdf
Takara
T202
RetroNectin¢ç GMP grade (Recombinant Human Fibronectin Fragment CH-296)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
2.5 mg
(2.5 ml)
2,247,200¿ø 
2,809,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
T202_DS.v1912.pdf





¡Ø RetroNectin¢ç GMP grade (Code T202) Á¦Ç° ÁÖ¹®½Ã Àü¿ë ÁÖ¹®¼­¸¦ ÀÛ¼ºÇÏ¿© ´ÙÄ«¶óÄÚ¸®¾Æ(support@takara.co.kr)·Î º¸³»ÁÖ¼¼¿ä.
¡Ø µ¿°á°ÇÁ¶ ÇüÅÂÀÇ RetroNectin¢ç GMP grade (Code T201, Á¾¸Å)ÀÌ solution ÇüÅ (Code T202) ·Î ´ëüµÇ¾ú½À´Ï´Ù.

  • Lentivirus ¿Í retrovirusÀÇ transduction enhancer
  • Polybrene ¶Ç´Â protamineÀÇ Àû¿ëÀÌ ¾î·Á¿î ¼¼Æ÷µµ ³·Àº µ¶¼ºÀ¸·Î °íÈ¿À² transduction ½ÇÇö
  • Hematopoietic cell°ú °°Àº suspension cellµµ °íÈ¿À² transduction °¡´É
  • T-cell È®´ë¹è¾ç¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ¾î CAR-T cell ¿¬±¸¿¡ ÃÖÀû
    - ±âÁ¸ T-cell È®´ë¹è¾ç¹ý ´ëºñ ¾ÐµµÀûÀÎ È®´ë¹è¾ç
    - T-cell È®´ë¹è¾ç Àû¿ë ¿¹: ±¹¹® (Korean)¿µ¹® (English)
Á¦Ç°¼³¸í
RetroNectin® (Recombinant Human Fibronectin CH-296)Àº ¨ç Cell-binding domain (C-domain, VLA-5¿Í °áÇÕ) /  ¨è Heparin-binding domain (H-domain, ¹ÙÀÌ·¯½º¿Í °áÇÕ) / ¨é CS-1 (VLA-4¿Í °áÇÕ), ÃÑ 3Á¾·ùÀÇ ±â´É¼º ´Ü¹éÁú ´ÜÆíÀ» °¡Áø human fibronectinÀÌ´Ù (±×¸² 1). ¸ñÀû ¼¼Æ÷°¡ VLA-4 ¶Ç´Â VLA-5¸¦ ¹ßÇöÇÏ¸é °¢°¢ CS-1 ¶Ç´Â Cell-binding domain°ú °áÇÕÇÏ°í À̶§ H-domain¿¡ °áÇÕÇÑ ¹ÙÀÌ·¯½º¿Í ¸ñÀû ¼¼Æ÷ÀÇ ¹°¸®Àû °Å¸®°¡ ÃÖ¼ÒÈ­µÇ´Â ¿ø¸®·Î transductionÀÌ ÃËÁøµÈ´Ù.


±×¸² 1. Fibronectin°ú RetroNectin®ÀÇ ±¸Á¶ ¹× transduction ¸ð½Äµµ ÀϹÝÀûÀ¸·Î Hematopoietic cells ¶Ç´Â ±× ¿ÜÀÇ suspension ¼¼Æ÷µéÀº transduction È¿À²ÀÌ ³·Áö¸¸ RetroNectin®Àº ³·Àº µ¶¼ºÀ¸·Î ÀÌ·± ¼¼Æ÷¿¡¼­µµ °íÈ¿À² transductionÀÌ °¡´ÉÇÏ´Ù. ÇöÀç, RetroNectin®Àº hematopoietic cellsÀÇ lentiviral / retroviral transduction¿¡ gold standard·Î »ç¿ëµÇ´Â enhancerÀ̸ç, 40°Ç ÀÌ»óÀÇ ÀÓ»ó ½ÃÇè¿¡ »ç¿ëµÇ¾î ÀÓ»ó Àû¿ë¿¡µµ Å« ±â´ë¸¦ ¹Þ°í ÀÖ´Ù.

¸ñÀû¼¼Æ÷·ÎÀÇ µµÀÔ¿¡´Â (1) supernatant¹ý ¶Ç´Â (2) RBV (RetroNectin-bound virus)¹ýÀ» Àû¿ëÇÒ ¼ö ÀÖ´Ù.

(1) Supernatant ¹ý
¼¼Æ÷¿Í ¹ÙÀÌ·¯½º¸¦ È¥ÇÕÇÏ¿© RetroNectin® ÄÚÆà Ç÷¹ÀÌÆ®¿¡ µµÀÔÇÏ´Â ¹æ¹ýÀ¸·Î ´Ü½Ã°£¿¡ °£ÆíÇÏ°Ô transductionÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù (¸ñÀû ¼¼Æ÷ ÷°¡ ÈÄ plate¸¦ ¿ø½ÉºÐ¸®ÇÏ¸é ¸ñÀû ¼¼Æ÷¿Í ¹ÙÀÌ·¯½ºÀÇ »óÈ£ÀÛ¿ëÀ» ÃËÁøÇÒ ¼ö ÀÖ´Ù.).


(2) RBV (RetroNectin-bound virus) ¹ý
¹ÙÀÌ·¯½º ¿ë¾×¿¡ packaging cell-line¿¡¼­ ºÐºñµÈ proteoglycanÀ̳ª envelope proteinÀÌ È¥ÀԵǴ °æ¿ì transductionÀ» ÀúÇØÇÏ´Â °æ¿ì°¡ ÀÖ´Ù. RBV¹ýÀº ÀÌ·¯ÇÑ ÀúÇع°ÁúÀ» Á¦°ÅÇÏ°í transductionÇÏ´Â ¹æ¹ýÀÌ´Ù. RetroNectin® ÄÚÆà Ç÷¹ÀÌÆ®¿¡ lentivirus ¶Ç´Â retrovirus¸¦ ¸ÕÀú °áÇÕ½ÃÅ°°í transduction inhibitor°¡ È¥ÀÔµÈ ¹èÁö¸¦ Á¦°ÅÇÑ ÈÄ, ¸ñÀû¼¼Æ÷¸¦ ÷°¡ÇÏ¿© transductionÇÑ´Ù. Supernatant¹ýÀ¸·Î ÃæºÐÇÑ transduction È¿À²ÀÌ ³ª¿ÀÁö ¾ÊÀ» °æ¿ì RBV¹ýÀ» ÃßõÇÏ¸ç °í³óµµ·Î ¹ÙÀÌ·¯½º¸¦ »ç¿ëÇÏ´Â °æ¿ì¿¡¼­ ³ôÀº È¿À²À» º¸Àδ٠(¹ÙÀÌ·¯½ºÀÇ titer°¡ ºÎÁ·ÇÑ °æ¿ì ¹ÙÀÌ·¯½º ¿ë¾× ÷°¡ ÈÄ plate¸¦ ¿ø½ÉºÐ¸®ÇÏ¸é ¹ÙÀÌ·¯½ºÀÇ transduction È¿À²À» ´õ¿í ³ôÀÏ ¼ö ÀÖ´Ù.).


* RetroNectin® Precoated Dish (Code T110A)´Â RetroNectin®ÀÌ 35 mm dish¿¡ ¹Ì¸® ÄÚÆÃµÈ ready-to-use dishÀÌ´Ù. ÄÚÆðúÁ¤À» »ý·«ÇÏ°í ÀÏÁ¤ÇÑ À¯ÀüÀÚ µµÀÔ È¿À²À» ¾òÀ» ¼ö ÀÖ´Ù.

* RetroNectin® GMP (Code T202)´Â GMP °¡À̵å¶óÀο¡ ÁØÇÑ Á¦Á¶ ¹× Ç°Áú °ü¸® °øÁ¤¿¡ ÀÇÇØ »ý»êµÈ GMP gradeÀÇ RetroNectin®À¸·Î ¿¬±¸¿ëÀ¸·Î ÆǸŵǴ RetroNectin® (Code T100A/B)º¸´Ù °íÇ°Áú·Î À¯ÀüÀÚÄ¡·á³ª ¼¼Æ÷ Á¶Á¦ µîÀÇ ÀÓ»ó ¿¬±¸¿¡ »ç¿ë °¡´ÉÇÏ´Ù.
¿ëµµ
- LA-4 ¶Ç´Â VLA-5¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷ (´Ù¾çÇÑ hematopoietic cell Æ÷ÇÔ)¿¡ lentivirus ¶Ç´Â retrovirusÀÇ µµÀÔ(Transduction enhancer)
- T-cell (CD8+) È®´ë¹è¾ç

Àû¿ë ¿¹

±×¸² 2. ´Ù¾çÇÑ Áٱ⼼Æ÷¿¡ ´ëÇÑ retrovirusÀÇ À¯ÀüÀÚ µµÀÔ È¿À² ºñ±³.
´Ù¾çÇÑ Áٱ⼼Æ÷¿¡ ¼¼ °³ÀÇ transduction enhancer¸¦ ÀÌ¿ëÇÏ¿© retrovirus¸¦ »ç¿ëÇÑ Çü±¤ ´Ü¹éÁúÀÇ ¹ßÇö È¿À²À» ºñ±³ÇÏ¿´´Ù. ±× °á°ú, RetroNectin®Àº GALV envelope receptorÀÇ ¹ßÇöÀÌ ³·Àº hCD34+, hADSC °°Àº Áٱ⼼Æ÷¿¡¼­µµ ³ôÀº È¿À²ÀÇ transductionÀÌ °¡´ÉÇÏ¿´´Ù.

Cell

Efficiency of Gene Transfer (%)

TF-1

53.3

HEL

28.1

NIH/3Y3

80.5

CD34+bMC

86.9

Mice BM MNC fraction

29.2

K-562

93.5

HL-60

89.4


Ç¥ 1. RetroNectin®À» »ç¿ëÇÑ transduction efficiency ¿¹½Ã


±×¸² 3. Lentivirus¸¦ »ç¿ëÇÑ suspension cell (SUP-T1) À¯ÀüÀÚ µµÀÔ »ç·Ê
suspension cellÀÎ SUP-T1¿¡ lentivirus¸¦ »ç¿ëÇÑ Çü±¤ ´Ü¹éÁúÀÇ ¹ßÇöÀ» ÀÌ¿ëÇÏ¿© transduction ¹æ¹ý º° È¿À²À» ºñ±³ÇÏ¿´´Ù. ±× °á°ú RetroNectin®À» »ç¿ëÇÑ Supernatant¹ý (static¹ý, spin¹ý), RBV-Spin¹ý ¸ðµÎ polybreneº¸´Ù ³ôÀº È¿À²·Î transductionÀÌ °¡´ÉÇÏ¿´´Ù.
Çü»ó

RetroNectin® (Recombinant Human Fibronectin Fragment) (Code T100A/B)

- 20¡É

RetroNectin® Dish (35 mm diameter Dish) (Code T110A)

4¡É

RetroNectin® GMP grade (Recombinant Human Fibronectin Fragment CH-296) (Code T202)

- 30¡É ÀÌÇÏ

±â¿ø
Human fibronectin fragment CH-296À» ¹ßÇöÇÏ´Â E.coli
¼øµµ
HPLC¿¡¼­ ¼øµµ 90% ÀÌ»ó.
»ç¿ë »óÀÇ ÁÖÀÇ
- Freeze/thaw´Â 10ȸ* Á¤µµ±îÁö °¡´ÉÇÏ´Ù.
* RetroNectin® GMP grade (Code T202)´Â Çѹø ¿ëÇØ ÈÄ room temperature¿¡¼­ 12½Ã°£ À̳» ¸ðµÎ »ç¿ëµÇ¾ß ÇÑ´Ù.
- °ú°ÝÇÑ ±³¹ÝÀº ÇÇÇÑ´Ù.
ºñ°í
RetroNectin®Àº lentivirus ¶Ç´Â retrovirusÀÇ À¯ÀüÀÚ µµÀÔ È¿À² Áõ°¡ »Ó¸¸ ¾Æ´Ï¶ó, T cellÀÇ È®´ë¹è¾ç¿¡µµ Àû¿ë °¡´ÉÇÔÀÌ È®ÀεǾú´Ù. ÀϹÝÀûÀ¸·Î T cell È®´ë ¹è¾çÀº Anti-CD3 antibody (OKT3)ÀÇ coating ÈÄ ¹è¾ç¾×¿¡ IL-2¸¦ ÷°¡ÇÏ¿© ½Ç½ÃÇÑ´Ù. À̶§, RetroNectin®À» Anti-CD3 antibody¿Í ÇÔ²² coatingÇÏ¸é ±âÁ¸ÀÇ È®´ë ¹è¾ç¹ý ´ëºñ ¾à 1.5¹è ÀÌ»ó È®´ë ¹è¾çÀÌ °¡´ÉÇÏ°í ³ôÀº naive T cell ºñÀ²À» À¯ÁöÇÒ ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀº CAR-T cell ¿¬±¸¿¡ Gold standard·Î »ç¿ëµÇ°í ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ³ôÀº °¨¿° È°¼ºÀ» °¡Áö´Â AAV/lentivirus »ý»êÀ» À§ÇÑ transfection ½Ã¾à TransIT-VirusGEN® Transfection Reagent (Code MIR6700) (GMP gradeµµ ÆǸŠÁß)
- Mycoplasma ¿À¿° °ËÃâ, luminometer¸¦ »ç¿ëÇÑ MycoAlert PLUS Mycoplasma detection kit (Code LT07-701)
- Mycoplasma ¿À¿° °ËÃâ, nested PCRÀ» »ç¿ëÇÑ TaKaRa PCR Mycoplasma Detection Set (Code 6601)
- Mycoplasma ¿À¿° °ËÃâ, Real-Time PCRÀ» »ç¿ëÇÑ CycleavePCR® Mycoplasma Detection Kit (Code CY232)

Keyword : T100,retronectin

T cell therapy ¿¬±¸
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Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
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T cell stimulation¿ë Ab
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LymphoONE¢ç T-Cell Expansion Xeno-Free Medium
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