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°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ

TransIT-VirusGEN¢ç Transfection Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 6700
TransIT¢ç-VirusGEN¢â Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1.5 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
transit-virusgen-transfection.pdf
Mirus
MIR 6703
TransIT¢ç-VirusGEN¢â Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.3 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
Mirus
MIR 6704
TransIT¢ç-VirusGEN¢â Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.75 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
Mirus
MIR 6705
TransIT¢ç-VirusGEN¢â Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 x 1.5 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
Mirus
MIR 6706
TransIT¢ç-VirusGEN¢â Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 x 1.5 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 §¢
°¡°Ý¹®ÀÇ   °¡°ÝÇÒÀÎ
05.02 ~ 06.30
transduceit-reagent-pds.pdf




  • ³ôÀº °¨¿°È°¼ºÀ» °¡Áö´Â °íǰÁúÀÇ Adeno associated virus (AAV), Lentivirus »ý»ê
  • AAV, Lentivirus titer Áõ°¡
  • HEK293 ¼¼Æ÷ ¹è¾ç ½Ã½ºÅÛ¿¡ ÃÖÀû (adherent ¹× suspension ¹è¾ç ¸ðµÎ Àû¿ë)
  • Animal Origin Free
Á¦Ç°¼³¸í
TransIT-VirusGEN¢ç Transfection Reagent´Â transfer vector¿Í packaging vector¸¦ HEK293 ŸÀÔÀÇ ÆÐŰ¡ ¼¼Æ÷·Î È¿À²ÀûÀ¸·Î µµÀÔÇÏ¿©, AAV ¶Ç´Â LentivirusÀÇ »ý»êÀ²À» ³ô¿©ÁÖ´Â transfection ½Ã¾àÀÌ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© AAV ¶Ç´Â Lentivirus¸¦ »ý»êÇϸé virusÀÇ ¿ª°¡¸¦ ³ô¿©ÁÙ »Ó¸¸ ¾Æ´Ï¶ó, °¨¿°´É·ÂÀÌ ³ôÀº °íǰÁúÀÇ ¹ÙÀÌ·¯½º (active virus)¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
TransduceIT¢â Transduction Reagent´Â hexadimethrine bromide À¸·Î, À̸¦ »ç¿ëÇϸé lentivirus ¶Ç´Â retrovirus transduction È¿À²À» ³ôÀÏ ¼ö ÀÖ´Ù.
*ÀÓ»ó½ÃÇèÀ» À§ÇÑ Viral vector ´ë·®»ý»ê¿ë transfection ½Ã¾àÀº TransIT-VirusGEN¢ç SELECT¸¦ È®ÀÎÇϼ¼¿ä.


±×¸²1. Ÿ»ç´ëºñ ³ôÀº AAV functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A)¿Í Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡ Trans IT-VirusGEN¢ç Transfection Reagent (reagent : DNA (vol : wt) = 2 : 1), A»çÁ¦Ç°1, A»çÁ¦Ç°2, 25kDa PEI¸¦ »ç¿ëÇÏ¿© pAAV-hrGFP, pAAV-RC, pAAV-Helper (1 : 1 : 1 ratio, 1.5 §¶/§¢, Agilent Technologies)¸¦ transfection ÇÏ¿© ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ¿´´Ù. »ý»êµÈ ¹ÙÀÌ·¯½º´Â HT1080¼¼Æ÷¿¡ °¨¿°, 48½Ã°£ µÚ GFP¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.


±×¸²2. Ÿ»ç´ëºñ ³ôÀº Lentivirus functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A), Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡ Trans IT-VirusGEN¢ç Transfection Reagent (reagent : DNA (vol : wt) = 3 : 1), A»çÁ¦Ç°1, A»çÁ¦Ç°2, 25kDa PEI¸¦ »ç¿ëÇÏ¿© pLKO.1-puro-CMV-TurboGFP transfer vector, MISSION Lentivirus Packaging Mix (1 : 1 ratio, 1 §¶/§¢)À» transfection ÇÏ¿´´Ù. TransfectionµÈ ¼¼Æ÷ÀÇ ¹è¾ç »óÃþ¾×À» 0.45 §­ ÇÊÅÍ·Î ¿©°úÇÏ¿© 8 §¶/§¢TransduceIT¢â °ú ÇÔ²² 293T/18¿¡ °¨¿°½ÃÄ×´Ù. 72½Ã°£ µÚ GFP¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.


±×¸²3. ´Ù¾çÇÑ AAV »ý»ê ½ºÄÉÀÏ¿¡ Àû¿ë °¡´É
Suspension ÇüÅ·Π¹è¾çÇÑ FreeStyle 293-F ¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A), Adherent ÇüÅ·Π¹è¾çÇÑ 293T / 17 ¼¼Æ÷(B)¿¡ Trans IT-VirusGEN¢ç Transfection Reagent¸¦ »ç¿ëÇÏ¿© pAAV -hrGFP, pAAV-RC, pAAV-Helper (1 : 1 : 1 ratio, 1.5 §¶/§¢, Agilent Technologies)¸¦ transfectionÇÏ¿© ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ¿´´Ù (reagent : DNA (vol : wt) = 2 : 1). »ý»êµÈ ¹ÙÀÌ·¯½º´Â HT1080 ¼¼Æ÷¿¡ °¨¿°, 48 ½Ã°£ µÚ¿¡ GFP ¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.
º¸Á¸ : - 20¡É
°ü·ÃÁ¦Ç°
- ´Ù¾çÇÑ SerotypeÀÇ vector system: AAVpro¢ç Helper Free System (AAV1, 2, 5, 6)
- CRISPR/Cas9 Vector System: AAVpro¢ç CRISPR/Cas9 Helper Free System (AAV2)
- AAV extraction: AAVpro¢ç Extraction Solution
- AAV purification: AAVpro¢ç Purification Kit (All Serotypes)
- AAV titration: AAVpro¢ç Titration Kit (for Real Time PCR)
- Packaging (293T Cell Line): AAVpro¢ç 293T Cell Line

Keyword : AAV,lentifirus,lenti,adeno associated,adeno,virus

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°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ
TransIT-VirusGEN¢ç Transfection Reagent
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Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
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(ÀüÀÓ»ó¿ë) °í¿ª°¡ÀÇ AAV, Lentivirus »ý»ê ½Ã..
TransIT-VirusGEN¢ç Transfection Reagent