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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Adeno-Associated Virus (AAV) > Purification Kit Maxi/Midi (All Serotypes)
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû

Purification Kit Maxi/Midi (All Serotypes)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6666
AAVpro¢ç Purification Kit Maxi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
4 ȸ
(Package)
859,200¿ø 
1,074,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6666_e.v1912Da.pdf
Takara
6675
AAVpro¢ç Purification Kit Midi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
4 ȸ
(Package)
360,800¿ø 
451,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6675_6675S_e.v2001Da.pdf

AAVpro¢ç Purification Kit Maxi (All Serotypes) (Code 6666)Àº cell »Ó¸¸ ¾Æ´Ï¶ó Sup.¿¡ ÀÖ´Â AAV±îÁö Çѹø¿¡ Á¤Á¦ÇÒ ¼ö ÀÖ´Â AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes) (Code 6676)·Î ¾÷±×·¹ÀÌµå µÇ¾ú½À´Ï´Ù.
ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é »ý»êµÈ AAV¸¦ ³²±è¾øÀÌ Á¤Á¦ÇØ ´õ¿í ³ôÀº ¼öÀ²À» ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
  • ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ °í¼øµµ·Î Á¤Á¦ °¡´É
  • º¹ÀâÇÏ°í ½Ã°£ÀÌ ¿À·¡ ¼Ò¿äµÇ´Â Ultracentrifuge °úÁ¤ ºÒÇÊ¿ä
  • µ¶ÀÚÀûÀÎ AAV ÃßÃâ¹ýÀ¸·Î Freeze/thaw, sonication ºÒÇÊ¿ä (ƯÇã Ãâ¿ø Áß)
  • µ¶ÀÚÀûÀÎ AAV Á¤Á¦¹æ¹ýÀ¸·Î cell lysate µîÀ» È¿À²ÀûÀ¸·Î Á¦°Å (ƯÇã Ãâ¿ø Áß)
  • Producing cell ¿¡¼­ÀÇ AAV ÃßÃâºÎÅÍ Á¤Á¦¿¡ ÇÊ¿äÇÑ ¸ðµç component Æ÷ÇÔ
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­, ºñºÐ¿­ ¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡ ´ëÇØ Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇϸç ƯÈ÷, ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÈ­µÈ virusÀÌ´Ù.
in-vivo ½ÇÇè¿¡ Àû¿ëÇÏ´Â °æ¿ì, µ¶¼ºÀ» °¨¼Ò½ÃÅ°±â À§ÇÏ¿© Packaging cell line ¶Ç´Â ¹èÁö À¯·¡ÀÇ ºÒ¼ø¹°À» Á¦°ÅÇÏ¿© °í¼øµµ·Î Á¤Á¦µÈ AAV¸¦ »ç¿ëÇÏ´Â °ÍÀº ¸Å¿ì Áß¿äÇÏ´Ù. ¶ÇÇÑ, culture cellÀÇ °æ¿ì¿¡µµ AAV¸¦ Á¤Á¦ÇÏ¿© »ç¿ëÇÏ¸é ºÒ¼ø¹°¿¡ ÀÇÇÑ ¿µÇâÀ» ÃÖ¼ÒÈ­ÇÒ ¼ö ÀÖ´Ù.

AAV Á¤Á¦¿¡´Â ÀϹÝÀûÀ¸·Î CsCl2/iodixanol gradient ultracentrifugation ¹ýÀÌ »ç¿ëµÇÁö¸¸ °íµµÀÇ ¼÷·ÃµÈ ±â¼úÀ» ÇÊ¿ä·Î Çϸç, ȸ¼öÀ²ÀÌ ³·°í, ½Ã°£ÀÌ ¿À·¡ ¼Ò¿äµÇ´Â ´ÜÁ¡ÀÌ ÀÖ´Ù.

º» Á¦Ç°Àº ¾à 4½Ã°£¸¸¿¡ ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ °£ÆíÇÏ°í È¿À²ÀûÀ¸·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Â kit·Î, µ¶ÀÚÀûÀÎ AAV extraction ¹æ¹ýÀ» »ç¿ëÇϹǷΠFreeze/thaw¹ý, sonication¹ý°ú °°ÀÌ ¹ø°Å·Î¿î Á¶ÀÛÀÌ ºÒÇÊ¿äÇÏ°í, ÀÚü °³¹ßÇÑ AAV Á¤Á¦¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© °£´ÜÇÏ°Ô ¼¼Æ÷, ¹èÁöÀ¯·¡ ºÒ¼ø¹°À» Á¦°ÅÇÏ°í °í¼øµµÀÇ AAV¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
AAVpro¢ç Purification Kit (All Serotypes)¿¡´Â T225 flask 5°³ ºÐ·®, AAVpro¢ç Purification Kit Midi (All Serotypes)¿¡´Â T225 flask 1°³ ºÐ·®ÀÇ producing cell¿¡¼­ AAV¸¦ Á¤Á¦Çϱâ À§ÇÑ buffer, concentration column 4ȸºÐÀ» Æ÷ÇÔÇÏ°í ÀÖ´Ù.

[Maxi kit¿Í Midi kit¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Â »ùÇþç]

1ȸ Á¤Á¦´ç
Àû¿ë°¡´ÉÇÑ »ùÇà ¾ç*

Code

Á¦Ç°¸í

¿ë·®

T225
flask

15 cm
dish

6666

AAVpro¢ç Purification Kit Maxi (All Serotypes)

4 purifications

x5

x7.5

6675

AAVpro¢ç Purification Kit Midi (All Serotypes)

4 purifications

x1

x1.5

*ÇØ´ç ºÐ·®ÀÇ AAV producing cellÀ» Á¤Á¦¿¡ »ç¿ë
*[¾È³»] ½ÅÁ¦Ç° AAVpro¢ç Purification Kit Midi (All Serotypes)ÀÇ Ãâ½Ã¿¡ µû¶ó ±âÁ¸ AAVpro¢ç Purification Kit (All Serotypes) (Code 6666)ÀÇ Á¦Ç°¸íÀÌ AAVpro¢ç Purification Kit Maxi (All Serotypes) ·Î º¯°æµÇ¾ú½À´Ï´Ù.
Á¦Ç°¸í ÀÌ¿Ü¿¡ Á¦Ç°ÄÚµå, ±¸¼ºÇ°, °¡°Ý, »ç¿ëȽ¼ö¿Í ¹æ¹ýÀº º¯°æµÇÁö ¾Ê¾ÒÀ¸´Ï ÀÌ¿ë¿¡ Âü°í ¹Ù¶ø´Ï´Ù.
Workflow
³»¿ë
   

Maxi
(Code 6666)

Midi
(Code 6675)

1.

AAV Extraction Solution A plus

22 §¢¡¿ 2°³

4.4 §¢¡¿ 2°³
2.

AAV Extraction Solution B

4.5 §¢

0.9 §¢
3.

Precipitator A*1

5 §¢

1 §¢
4.

Precipitator B

2.5 §¢

0.5 §¢
5.

Millex-HV 0.45¥ìm

4°³

4°³
6.

Amicon Ultra-15 ¶Ç´Â -4, 100 kDa

4°³

4°³
7. Suspension Buffer

60 §¢¡¿ 2°³

12 §¢¡¿ 2°³
8. Cryonase Cold-active Nuclease

500 §¡

100 §¡

*1 Àú¿Â º¸Á¸¿¡ ÀÇÇØ ¹é»ö ħÀü¹°ÀÌ »ý±â´Â °æ¿ì, 37¡É¿¡¼­ ¿ÏÀüÈ÷ ¿ëÇØ ÈÄ »ç¿ëÇϼ¼¿ä
º¸Á¸

Cryonase Cold-active Nuclease

-20¡É

±× ¿Ü

½Ç¿Â

AAVpro¢ç Purification Kit Maxi (All Serotypes) Àû¿ë ¿¹ - ´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦ ¹× °¨¿° ´É·Â È®ÀÎ
1) AAV Á¤Á¦ ¹× ¼øµµ ÃøÁ¤
T225 flask 5°³ ºÐ·®ÀÇ producing cell·ÎºÎÅÍ º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© Çü±¤´Ü¹éÁú ZsGreen 1À» Æ÷ÇÔÇÏ´Â ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ Á¤Á¦ÇÏ¿´´Ù. AAVpro¢ç Titration Kit (for Real Time PCR)·Î Á¤Á¦µÈ AAVÀÇ ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿© 1 x109 vg/laneÀ¸·Î SDS-PAGE¸¦ ¼öÇàÇÏ¿´´Ù. ±× °á°ú AAV Capsid ´Ü¹éÁú VP1, VP2, VP3 band¸¦ È®ÀÎÇÏ¿´´Ù.



2) Á¤Á¦µÈ AAVÀÇ °¨¿° È¿À² ºñ±³
Á¤Á¦ ÈÄ °¢ serotypeÀÇ AAV¸¦ ÀÌ¿ëÇÏ¿© cell lineº° °¨¿° ¿ª°¡¸¦ È®ÀÎÇÏ¿´´Ù.
Á¤Á¦µÈ AAV¸¦ 5,000 vg/cell (serotype 1, 2, 3, 6) ¶Ç´Â 50,000 vg/cell (serotype 5)À¸·Î °¨¿°½ÃÅ°°í 3ÀÏ ÈÄ¿¡ ÈÄ·Î cytometry Çؼ®À» ½Ç½ÃÇÑ °á°ú, º» Á¦Ç°À¸·Î Á¤Á¦ÇÑ AAV´Â °¨¿°´É·ÂÀ» º¸À¯ÇÏ°í ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.

°ü·ÃÁ¦Ç°

Keyword : Adeno Associated Virus,in vivo,aav virus

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
>
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
>
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
>
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
>
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
>
Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
>
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
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>
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
>
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
>
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
>
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
>
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution