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Gene transfer (virus)
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Lentivirus
> Packaging Cell Lines
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿À²
Packaging Cell Lines
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(ºÎ°¡¼¼º°µµ)
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Clontech
632180
Lenti-X¢â 293T Cell Line
1 ml
737,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
[ÁÖÀÇ]
µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE:
We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
°í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º Á¦ÀÛÀ» À§ÇØ Á¦ÀÛµÈ 293T Clone
293FT Cell LineÀÇ 6¹è, HEK293 Cell Line¿¡ ºñÇÏ¿© 30¹è ¿ª°¡°¡ ³ôÀº ¹ÙÀÌ·¯½º »ý»ê
´Ù¾çÇÑ Á¾·ùÀÇ ·»Æ¼¹ÙÀÌ·¯½º º¤ÅÍ¿¡ Àû¿ë °¡´É
Lenti-X¢â Packaging single shots (Code 631275)
¿Í ÇÔ²² »ç¿ëÇϸé 10^8 IFUÀÇ ¹ÙÀÌ·¯½º¸¦ Á¦ÀÛ °¡´É (10cm plate ±âÁØ)
Á¦Ç°¼³¸í
Lenti-X¢â 293T Cell LineÀº °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ Á¦ÀÛ °¡´ÉÇÑ ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡¿ë ¼¼Æ÷ÁÖÀÌ´Ù. º» ¼¼Æ÷ÁÖ¸¦ ÀÌ¿ëÇϸé ÀϹÝÀûÀ¸·Î »ç¿ëÇÏ´Â 293FT Cell Line°ú HEK293 Cell LineÀ» ÀÌ¿ëÇÏ¿´À» ¶§º¸´Ù ¾à 6¹è-30¹è ¿ª°¡°¡ ³ôÀº ¹ÙÀÌ·¯½º¸¦ ¾òÀ» ¼ö ÀÖ´Ù (±×¸² 1)
±×¸² 1. Lenti-X¢â 293T Cell LineÀÇ ¶Ù¾î³ ¹ÙÀÌ·¯½º »ý»ê ´É·Â
We used our 4th generation lentiviral packaging system and one of our pLVX lentiviral vectors to compare the virus production of the Lenti-X 293T Cell Line to that of two other commonly used HEK 293-based cell lines. Lenti-X 293T cells clearly outperformed the other cell lines?producing over 6 times more virus than 293FT cells and up to 30 times more virus than the parental HEK 293 cell line.
½Ã³ÊÁö È¿°ú : ³ôÀº ¿ª°¡¸¦ À§ÇÑ ÇØ´ä
¾Æ·¡ÀÇ ±¸¼º¿ä¼Ò¸¦ 293T ¼¼Æ÷¿Í ÇÔ²² »ç¿ëÇϸé ClontechÀÇ Lenti-X System Vector »Ó¸¸ ¾Æ´Ï¶ó ´Ù¸¥ lentivirus vector¸¦ ÀÌ¿ëÇÒ ¶§µµ ÃÖ°í ¿ª°¡ÀÇ ¾ÈÀüÇÑ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
ÃÖÀûÈµÈ Packaging Mix - ClontechÀÇ 4¼¼´ë packaging mixÀÎ Lenti-X¢â Packaging Single Shots´Â °í¿ª°¡ÀÇ Lentivirus¸¦ °£´ÜÇÏ°í È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï ÃÖÀûÈ µÇ¾î ÀÖ´Ù. °¢°¢ÀÇ packaging vector¿Í transfection ½Ã¾àÀÌ ¸ðµÎ ÃÖÀûÀÇ ºñÀ²·Î È¥ÇÕµÇ¾î µ¿°á°ÇÁ¶ »óÅ·ΠÁ¦°øµÇ¸ç, lentiviral expression vectorÀÇ Ã·°¡¸¸À¸·Î ¾ÆÁÖ ¼Õ½±°Ô packagingÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
Tet System Transactivation - Tetracycline-responsive promoter element(TRE)ÀÇ Tet-Off transactivationÀ» ÅëÇÏ¿© packaging¿¡ ÇÊ¿äÇÑ ¸î °¡Áö ÁÖ¿ä ±¸¼º¿ä¼ÒµéÀ» °íµµ·Î ¹ßÇö½ÃŲ´Ù. HEK293 ±â¹ÝÀÇ cell lines¿¡¼ Tet System À¯µµ¹ßÇöÀº CMV promoter¿¡ ÀÇÇØ »ý»êµÇ´Â ´Ù¸¥ ½Ã½ºÅÛ¿¡¼ º¸´Ù ÈξÀ ³ôÀº ¹ßÇöÀ» À̲ø¾î³½´Ù (1).
ÃÖÀûÈµÈ transfection - xfect´Â °íÈ¿À²ÀÇ ºñµ¶¼º transfectionÀ» °¡´ÉÇÏ°Ô Çϸç, lipid-based transfection ¹ýº¸´Ù ´õ ³ôÀº ¹ßÇöÀ» À¯µµÇÑ´Ù. Lentiphos HT´Â 293T packaging cells¿¡ ÃÖÀûȵǾî ÀÖÀ¸¸ç, 99% ÀÌ»óÀÇ transfection È¿À²À» ¾òÀ» ¼ö ÀÖ´Ù. ÃÖÀûÈµÈ pH¿Í buffer Á¶¼º, ¿°³óµµ, ¹ÝÀÀ ¿ë·®°ú ÇÔ²² °£´ÜÇÑ ½ÇÇè Á¶ÀÛ °úÁ¤Àº ÃÖÀûÀÇ transfectionÀ» º¸ÀåÇÑ´Ù.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº COA¸¦ ÂüÁ¶Çϼ¼¿ä)
1ml Lenti-X 293T Cell Line (2.0 x 10
6
cells/tube)
º¸Á¸
Liquid nitogren (-196 ¡É)
¶Ç´Â Á¦Ç° ¼ö·É ÈÄ, ¹Ù·Î À¶ÇØ ¹× ¹è¾ç ±ÇÀå
Shipping
Dry ice (-80 ¡É)
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
Yin, D. X. et al. (1996) Anal. Biochem. 235:195-201.
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Keyword :
Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System