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Transfection 시약을 포함하여 더 간단한 packaging vector mix

Packaging single shots

제조사	제품코드	제품명	용량	가격 (부가세별도)	사용자매뉴얼
Clontech	631275	Lenti-X™ Packaging Single Shots (VSV-G)	16 회	1,350,000원	,
Clontech	631276	Lenti-X™ Packaging Single Shots (VSV-G)	96 회	3,779,000원	,
Clontech	631282	Lenti-X™ Packaging Single Shots (96-well, VSV-G)	4 x 96-well plates	1,812,000원
Clontech	631277	Lenti-X™ Packaging Single Shots (Integrase Deficient)	16 회	1,658,000원
Clontech	631278	Lenti-X™ Packaging Single Shots (Ecotropic)	16 회	1,658,000원

Product index

* Lenti-X™ Packaging Single shots은 기존의 Lenti-X™ HTX Packaging System의 업그레이드 제품입니다.

convenience with high performance
Consistently high titers (10⁷-10⁸ IFU/ml)
Simple protocol-Just add your lentiviral DNA, mix, and add to 293T cells
No optimization or additional transfection reagent required

Lenti-X™ Packaging Single Shots (VSV-G)는 간단하고 효율적으로 고역가의 렌티바이러스를 제작할 수 있는 Clontech의 4세대 Lentiviral packaging system이다. Lenti-X™ Packaging Single Shots (VSV-G)는 VSV-G 타입의 pseudotype Lenti-X™ Lentivral packaging plasmid에 최적화되어있는 Xfect™ Transfection reagent가 미리 포함된, pre-aliquoted, lyophilized 시약이기 때문에 실험 과정 중에서 trasnfection reagent를 따로 사용하지 않아도 되는 편리한 제품이다.
또한, single tube 형태와 96 well plate 형태를 선택할 수 있어, high-throughput이 요구되는 실험에도 적용할 수 있다.

그림 1. The Lenti-X™ Packaging Single Shots (VSV-G) protocol.

그림 2. Lenti-X™ Packaging Single Shots (VSV-G) consist of single tubes of lyophilized Xfect™ Transfection Reagent premixed with VSV-G pseudotyped lentiviral packaging plasmids. To produce high-titer lentivirus, simply add your lentiviral vector in sterile water to a tube, mix, and add to 293T cells.

그림 3. Consistent, high-efficiency transfections lead to high titers. A lentiviral vector containing the ZsGreen1 gene was packaged according to the Lenti-X™ Single Shots protocol in four independent experiments. Briefly, 7 μg of pLVX-ZsGreen1 plasmid was added to each of four Lenti-X™ Single Shots; the tubes were vortexed for 20 sec and incubated at room temperature for 10 min. Then, the mixture was added to cultured Lenti-X™ 293T cells that were approximately 80% confluent. Forty-eighty hours after transfection, the cells were imaged by fluorescence microscopy (Panel A, top) and light microscopy (Panel A, bottom). After images were taken, the supernatant was harvested and used infect HT1080 cells for titer determination (Panel B, IFU/ml).

적용

- Lentiviral transduction of a broad range of hard-to-transfect cell types
- Easily create stable cell lines
- Create VSV-G pseudotyped lentivirus

구성품 (자세한 내용은 CoA를 참조하세요)

* Lenti-X™ Packaging Single Shots (VSV-G) (Code 631275)
   1 box containing 16 Lenti-X™ Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X™ Packaging Single Shots (VSV-G) (Code 631276)
   1 box containing 96 Lenti-X™ Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X™ Packaging Single Shots (96-well, VSV-G) (Code 631282)
   4 x Lenti-X Packaging Single Shots 96-well Plate (VSV-G) in foil pouches

* Lenti-X™ Packaging Single Shots (Ecotropic) (Code 631277)
   1 box containing 16 Lenti-X Packaging Single Shots (Ecotropic), in a foil pouch

* Lenti-X™ Packaging Single Shots (Integrase deficient) (Code 631278)
   1 box containing 16 Lenti-X™ Packaging Single Shots (Integrase Deficient), in a foil pouch

Keyword : Lentivirus,Lenti-virus,packaging,Lentiviral,Lentivral delivery,Lenti,soeasy,so easy

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