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Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packaging vector mix

Packaging single shots

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631275
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,350,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778, x32785
Clontech
631276
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
3,779,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631282
Lenti-X¢â Packaging Single Shots (96-well, VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
4 x 96-well plates
1,812,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x110886
Clontech
631277
Lenti-X¢â Packaging Single Shots (Integrase Deficient)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,658,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631278
Lenti-X¢â Packaging Single Shots (Ecotropic)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,658,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778

* Lenti-X¢â Packaging Single shotsÀº ±âÁ¸ÀÇ Lenti-X¢â HTX Packaging SystemÀÇ ¾÷±×·¹À̵å Á¦Ç°ÀÔ´Ï´Ù.
  • convenience with high performance
  • Consistently high titers (107-108 IFU/ml)
  • Simple protocol-Just add your lentiviral DNA, mix, and add to 293T cells
  • No optimization or additional transfection reagent required

Lenti-X¢â Packaging Single Shots (VSV-G)´Â °£´ÜÇϰí È¿À²ÀûÀ¸·Î °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ClontechÀÇ 4¼¼´ë Lentiviral packaging systemÀÌ´Ù. Lenti-X¢â Packaging Single Shots (VSV-G)´Â VSV-G ŸÀÔÀÇ pseudotype Lenti-X¢â Lentivral packaging plasmid¿¡ ÃÖÀûÈ­µÇ¾îÀÖ´Â Xfect¢â Transfection reagent°¡ ¹Ì¸® Æ÷ÇÔµÈ, pre-aliquoted, lyophilized ½Ã¾àÀ̱⠶§¹®¿¡ ½ÇÇè °úÁ¤ Áß¿¡¼­ trasnfection reagent¸¦ µû·Î »ç¿ëÇÏÁö ¾Ê¾Æµµ µÇ´Â Æí¸®ÇÑ Á¦Ç°ÀÌ´Ù.
¶ÇÇÑ, single tube ÇüÅÂ¿Í 96 well plate ÇüŸ¦ ¼±ÅÃÇÒ ¼ö ÀÖ¾î, high-throughputÀÌ ¿ä±¸µÇ´Â ½ÇÇè¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.



±×¸² 1. The Lenti-X¢â Packaging Single Shots (VSV-G) protocol.



±×¸² 2. Lenti-X¢â Packaging Single Shots (VSV-G) consist of single tubes of lyophilized Xfect¢â Transfection Reagent premixed with VSV-G pseudotyped lentiviral packaging plasmids. To produce high-titer lentivirus, simply add your lentiviral vector in sterile water to a tube, mix, and add to 293T cells.



±×¸² 3. Consistent, high-efficiency transfections lead to high titers. A lentiviral vector containing the ZsGreen1 gene was packaged according to the Lenti-X¢â Single Shots protocol in four independent experiments. Briefly, 7 ¥ìg of pLVX-ZsGreen1 plasmid was added to each of four Lenti-X¢â Single Shots; the tubes were vortexed for 20 sec and incubated at room temperature for 10 min. Then, the mixture was added to cultured Lenti-X¢â 293T cells that were approximately 80% confluent. Forty-eighty hours after transfection, the cells were imaged by fluorescence microscopy (Panel A, top) and light microscopy (Panel A, bottom). After images were taken, the supernatant was harvested and used infect HT1080 cells for titer determination (Panel B, IFU/ml).
Àû¿ë
- Lentiviral transduction of a broad range of hard-to-transfect cell types
- Easily create stable cell lines
- Create VSV-G pseudotyped lentivirus
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
* Lenti-X¢â Packaging Single Shots (VSV-G) (Code 631275)
   1 box containing 16 Lenti-X¢â Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X¢â Packaging Single Shots (VSV-G) (Code 631276)
   1 box containing 96 Lenti-X¢â Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X¢â Packaging Single Shots (96-well, VSV-G) (Code 631282)
   4 x Lenti-X Packaging Single Shots 96-well Plate (VSV-G) in foil pouches

* Lenti-X¢â Packaging Single Shots (Ecotropic) (Code 631277)
   1 box containing 16 Lenti-X Packaging Single Shots (Ecotropic), in a foil pouch

* Lenti-X¢â Packaging Single Shots (Integrase deficient) (Code 631278)
   1 box containing 16 Lenti-X¢â Packaging Single Shots (Integrase Deficient), in a foil pouch




Keyword : Lentivirus,Lenti-virus,packaging,Lentiviral,Lentivral delivery,Lenti,soeasy,so easy

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