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Gene transfer (virus)
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Retrovirus
> Retrovirus vector £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
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Clontech
631508
Retro-X System
1 Set
(Package)
1,366,400¿ø
1,708,000¿ø
11.01 ~ 12.27
,
,
,
Clontech
631509
pLXSN Retroviral Vector
20 ¥ìg
662,400¿ø
828,000¿ø
11.01 ~ 12.27
,
Clontech
631503
pLNCX2 Retroviral Vector
20 ¥ìg
641,600¿ø
802,000¿ø
11.01 ~ 12.27
,
Clontech
631511
LRCX Retroviral Vector Set
1 Set
868,800¿ø
1,086,000¿ø
11.01 ~ 12.27
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
RetroNectin¢ç Pro (Rec..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Retro-X system
Retrovirus ¸Å°³¼º À¯ÀüÀÚ µµÀÔ¿¡ ÀÇÇØ °ÅÀÇ 100 %ÀÇ ¼¼Æ÷¿¡ ÇüÁú µµÀÔ
µµÀÔÀÌ °ï¶õÇÑ ¼¼Æ÷ÁÖ¿¡µµ È¿À²ÁÁ°Ô µµÀÔ
¾ÈÁ¤µÈ ¼¼Æ÷ÁÖ¸¦ °£´ÜÈ÷ Á¦ÀÛ
Retro-X systemÀ» »ç¿ëÇÏ¸é º¹Á¦´ÉÀÌ ¾ø´Â °¨¿°¼º retrovirus¸¦ È¿À²ÁÁ°Ô »ý¼ºÇÏ°í in vitro ¹× in vivo·Î ±¤¹üÀ§ÇÑ Æ÷À¯·ù ¼¼Æ÷¿¡ ¸ñÀûÀ¯ÀüÀÚ¸¦ µµÀÔÇÒ ¼ö ÀÖ´Ù. Retrovirus°¡ Áö´Ñ ÇüÁúµµÀÔ´ÉÀº »ó´çÈ÷ È¿À²ÀÌ ³ô¾Æ °ÅÀÇ ¸ðµç ºÐ¿¼¼Æ÷ÀÇ host genome ¼Ó¿¡ ¸ñÀûÀ¯ÀüÀÚ¸¦ ¾ÈÁ¤ÀûÀ¸·Î µµÀÔÇÒ ¼ö ÀÖ´Ù(1, 2).
Retro-X system & vectors
Retro-X system¿¡´Â ¸ð·Î´Ï »ýÁã ¹éÇ÷º´ virusÀÇ 3 Á¾·ù retrovirus ¹ßÇö vector¿Í RetroPack PT67ÀÌ Æ÷ÇԵǾî ÀÖ´Ù. pLNCX2¿Í pLXSNÀº ±âº»ÀûÀÎ retrovirus vector·Î, ´Ù¸¥ promoter¿¡¼ ¸ñÀûÀ¯ÀüÀÚ¸¦ ¹ßÇö½Ãų ¼ö ÀÖ´Ù. pLAPSNÀº ¼¼±ÕÀÇ ¾ËÄ®¸®¼º phosphatase À¯ÀüÀÚ¸¦ pLXSN¿¡ µµÀÔÇÏ¿© Á¦ÀÛÇÑ control reporter vectorÀÌ´Ù.
Q vector setÀÇ pQCXIN°ú pQCXIX´Â ÀÚ±âºÒÈ°È retrovirus vectorÀÌ´Ù. °í¿ª°¡¿Í È®½ÇÇÑ ¹ßÇö levelÀ» ½ÇÇöÇÏ°í promoter °£¼· °¡´É¼ºÀ» ÁÙÀ̵µ·Ï ¼³°èµÇ¾î ÀÖ´Ù.
RetroPack PT67 packaging ¼¼Æ÷ÁÖ
RetroPack PT67 cell lineÀº NIH/3T3ÀÇ packaging ¼¼Æ÷ÁÖ·Î, 10A1 virusÀÇ envelope ´Ü¹éÁúÀ» ¹ßÇöÇÑ´Ù. PT67 ¼¼Æ÷·Î packageÇÑ ¼¼Æ÷´Â 2 Á¾·ùÀÇ ´Ù¸¥ Ç¥¸éºÐÀÚ, Áï amphotropic retrovirus ¼ö¿ëü¿Í CALV ¼ö¿ëü¸¦ ÅëÇØ ¼¼Æ÷¿¡ ħÀÔÇÏ¿© ´Ù¸¥ ¼¼Æ÷ÁÖ·Î packageÇÑ virusº¸´Ùµµ ±¤¹üÀ§ÇÑ ¼÷ÁÖ¿¡ °¨¿°µÈ´Ù(4, 5).
RetroPack PT67 cell line, EcoPack 2-293 cell line, AmphoPack-293 cell line, Retro-X vectors´Â º°µµ·Î ±¸ÀÔÇÒ ¼ö ÀÖ´Ù.
Retro-X universal packaging system
Retro-X universal packaging systemÀº GP2-293 packaging ¼¼Æ÷ÁÖ¸¦ Ư¡À¸·Î Çϸç(6) packagingÇÑ virusÀÇ ¼÷ÁÖ¿ªÀ» Ç¥Àû ¼¼Æ÷ÁÖ¿¡ ÇÕÄ¥ ¼ö ÀÖ´Ù. Envelope ´Ü¹éÁúÀ» selectionÇÏ¿© retrovirus ¹ßÇö vector¿Í ÇÔ²² cotransfectÇÑ´Ù. 48½Ã°£ À̳»¿¡ °í¿ª°¡ÀÇ amphotropic, ecotropic, dueltropic ¶Ç´Â pantropicÇÑ virus ÀÔÀÚ¸¦ ¾òÀ» ¼ö ÀÖ´Ù(7).
±¸¼º¹°
Retro-X system ±¸¼º¹°
RetroPack PT67 cell line
pLNCX2 retroviral vector
pLXSN retroviral vector
pLAPSN retroviral vector
LNCX Seq/PCR primers
LXSN Seq/PCR primers
User manual(PT3132-1)
Retro-X universal packaging system ±¸¼º¹°
GP2-293 packaging cell line
p10A1 vector
pAmpho vector
pEco vector
pVSV-G vector
pQCLIN control vector
User manual(PT3132-1)
Vector information packets(PT3343-5, PT3748-5, PT3749-5, PT3750-5)
º¸°ü
-196 ¡É : RetroPack PT67, GP2-293 packaging cell lines
-20 ¡É : ÀÌ¿ÜÀÇ ¸ðµç ½Ã¾à
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
Âü°í¹®Çå
1. Miller, A. D. & Rosman, G. J.(1989) Bio techniques 7:980-990.
2. Miller, D. G., et al.(1994) Proc. Natl. Acad. Sci. USA 91:78-82.
3. Retro-X Q vectors(2002) Clontechniques ¥¹¥¶(3):13-14.
4. Miller, D. G. & Miller, A. D.(1994) J. Virol. 68:8270-8276.
5. Miller, A. D.(1996) Proc. Natl. Acad. Sci. USA 93:11407-11413.
6. Viral systems overview(1999) Clontechniques ¥¹¥³(2):20-21.
7. Retro-X universal packaging system(October 2003) Clontechniques ¥¹¥·(4):16-17.
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Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit