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Lentivirus
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½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer) °£ÀÌ ÃøÁ¤ (Instant test)
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Clontech
631280
Lenti-X¢â GoStix¢â Plus
20 ȸ
1,009,000¿ø
Clontech
631281
Lenti-X¢â GoStix¢â Plus
50 ȸ
2,040,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
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Adenovirus
Adeno-X¢â workflow
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Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
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qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
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EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
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pIRES Bicistronic Expr..
* º» Á¦Ç°Àº Lenti-X¢â GoStix (Code 631243)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
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Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
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Lentivirus »óµî¾×À» ȸ¼öÇϱâ À§ÇÑ ÃÖÀûÀÇ ½Ã°£ °áÁ¤
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Lenti-X¢â GoStix¢â Plus´Â Lentivirus »óµî¾×À¸·ÎºÎÅÍ ¾ÆÁÖ ºü¸£°í, °£´ÜÇÏ°í È¿À²ÀûÀ¸·Î Lentivirus ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Â °£ÀÌ ¿ª°¡ ÃøÁ¤±âÀÌ´Ù. Lenti-X¢â GoStix¢â Plus¸¦ ÀÌ¿ëÇÑ ¿ª°¡ ÃøÁ¤Àº GoStix Ä«¼¼Æ®¿¡ Lentivirus »óÃø¾× 20§¡¸¦ °¡ÇÏ°í, ¾à 10ºÐ ÈÄ Lentiviral p24ÀÇ ¿©ºÎ¸¦ ¹êµåÀÇ À¯¹«·Î È®ÀÎÇÑ´Ù (±×¸² 1, 2). ÀÌÀü ¹öÀüÀÇ GoStix Á¦Ç°¿¡ ºñÇÏ¿© Lenti-X¢â GoStix¢â Plus´Â ½º¸¶Æ®Æù ¾îÇÃÀ» ÅëÇÏ¿© ¹êµå °µµ¸¦ ºñ±³ ¹× ¼öÄ¡ÈÇÔÀ¸·Î½á ·»Æ¼¹ÙÀÌ·¯½ºÀÇ ´ë·«ÀûÀÎ ¿ª°¡¸¦ °£´ÜÇÏ°Ô ÃøÁ¤, ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
Lenti-X¢â GoStix¢â Plus ¾îÇÃÀ» ÀÌ¿ëÇÏ¿© µµÃâµÈ ¿ª°¡ (GoStix Value ¶Ç´Â GV)´Â ELISA ¹æ¹ýÀÇ °á°ú¿Í À¯»çÇÏÁö¸¸ ELISA º¸´Ù ´õ ½±°í °£´ÜÇÏ°Ô ¿ª°¡¸¦ ÃøÁ¤(±×¸² 6)ÇÒ ¼ö ÀÖÀ¸¸ç, °¢°¢ÀÇ ½ÇÇè (Batch)À¸·ÎºÎÅÍ ³ª¿Â LentivirusÀÇ ¾çÀ» ºñ±³ ºÐ¼®ÇÏ°í, ¹ÙÀÌ·¯½º¸¦ ȸ¼öÇϴµ¥ ÀûÀýÇÑ ½Ã°£ (virus harvest time)À» °áÁ¤ÇÏ´Â µ¥¿¡ ÃÖÀûÀÌ´Ù (±×¸² 4).
Lenti-X GoStix ¾îÇà ´Ù¿î·Îµå
Lenti-X GoStix PlusÀÇ »ç¿ë ¹æ¹ý
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Applying lentiviral supernatants to Lenti-X GoStix Plus cassettes
Lenti-X GoStix Plus ½º¸¶Æ®Æù ¾îÇà »ç¿ë ¿¹¿Í GoStix Value(GV) µµÃâ
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Using the Lenti-X GoStix App to analyze the test results
±×¸² 1. Lenti-X GoStix Plus´Â Lentivirus capsid¿¡ À§Ä¡ÇÑ p24 ´Ü¹éÁúÀ» °ËÃâÇÑ´Ù.
±×¸² 2. Lenti-X GoStix Plus¸¦ ÀÌ¿ëÇϸé 10ºÐ¸¸¿¡ LentivirusÀÇ ¿ª°¡¸¦ µµÃâ °¡´ÉÇÏ´Ù
±×¸² 3. Lenti-X GoStix PlusÀÇ ½ÇÇè °á°ú
Control band(C)´Â Ç×»ó °ËÃâÀÌ µÇ¸ç, Test band(T)°¡ ³ªÅ¸³µ´Ù´Â °ÍÀº »óÃþ¾×¿¡ ÃæºÐÇÑ Lentivirus°¡ Á¸ÀçÇÑ´Ù´Â °ÍÀ» ÀǹÌÇÑ´Ù
±×¸² 4. GoStix Value(GV) ¹× p24 »ý»ê·®°ú ¹ÙÀÌ·¯½º ȸ¼ö½Ã°£ÀÇ »ó°ü°ü°è
Lenti-X Single ShotsÀ» ÀÌ¿ëÇÏ¿© ZsGreen1 Lentivirus¸¦ 2 ¹Ýº¹ (duplicate)·Î Áغñ ÈÄ, ¹ÙÀÌ·¯½º ȸ¼ö½Ã°£¿¡ µû¶ó Infection unit°ú GV Value (GV)¸¦ ÃøÁ¤ÇÏ¿´´Ù.
±×¸² 5. Lenti-X GoStix Plus¸¦ ÀÌ¿ëÇÑ GoStix Value´Â ´Ù¾çÇÑ ¹ÙÀÌ·¯½º ½Ã½ºÅÛ¿¡ µû¶ó ½Å·Ú¼º ÀÖ´Â °á°ú¸¦ ³ªÅ¸³½´Ù.
°¢±â ´Ù¸¥ Lentiviral vector¿Í packaging systemÀ» ÀÌ¿ëÇÏ¿© ZsGreen1 Lentivirus¸¦ Á¦ÀÛ ÈÄ, Infection unit°ú GoStix Value(GV)¸¦ ÃøÁ¤ÇÏ¿´´Ù.
±×¸² 6. Lenti-X GoStix Plus¸¦ ÀÌ¿ëÇÑ GoStix Value(GV)´Â ELISA ¹æ½ÄÀÇ ¿ª°¡ ÃøÁ¤¹ý°ú À¯»çÇÏ´Ù
ZsGreen1 LentivirusÀÇ ¿ª°¡¸¦ ELISA ¹æ½ÄÀÇ Lenti-X p24 Rapid Titer Kit°ú Lenti-X GoStix Plus·Î ÃøÁ¤ÇÏ°í transduction È¿À²À» FACS·Î ºÐ¼®ÇÏ¿´´Ù.
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Confirm successful lentiviral packaging reactions
Compare virus amounts between different preparations
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Lenti-X GoStix Plus
Chase Buffer
p24 Control
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Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System