Lenti-X 선택가이드
Lentiviral product selection guide
Use the list below to choose the right lentiviral product for your research:
Lentiviral vectors
Lentiviral vector selection guideLearn more about the core elements included in all of our lentiviral vectors and see schematics of each vector type. |
CMV promoter vectorsExpress high levels of your transgene from a strong CMV promoter. |
EF-1 alpha promoter vectorsExpress your gene of interest from an EF-1 alpha promoter in all cell types, including those in which CMV promoters are often silenced, such as hematopoietic cells and stem cells. |
All-in-one tetracycline-inducible expression systemsSingle-vector systems to easily generate inducible expression constructs with high-fold induction. |
Vectors to control protein levels (ProteoTuner)Clone your gene downstream of a ProteoTuner destabilization domain (DD) and rapidly control the level of your protein with Shield1 or Guard1. |
Fluorescent protein expression vectorsMonitor expression and transduction efficiency with bright red, cyan, and green fluorescent proteins. |
IRES bicistronic vectorsCoexpress your transgene and an antibiotic or fluorescent marker using IRES-containing lentiviral vector systems. |
Inducible protein dimerization vectors (iDimerize)Fuse DmrB domains to your protein(s) of interest and induce homo- or heterodimerization with dimerizing ligands. |
Promoter reporter vectors (luciferase)Monitor the activity of your favorite promoter using a secreted luciferase. |
Promoterless reporter vectors (fluorescent protein)Monitor activity of your favorite promoter using a destabilized cyan, green, or red fluorescent protein. |
Cell-capture vectors (CherryPicker)Capture a homogeneous cell population on beads, based on gene expression or promoter activity. |
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Lentiviral packaging systems and cell line
High-titer lentiviral packaging system—pantropic (VSV-G) pseudotypeObtain titers >107 IFU/ml. If you are not currently getting at least 107 IFU/ml, you need Lenti-X Packaging Single Shots. |
High-titer lentiviral ecotropic packaging systemGet the same high titers, but with virus that has an ecotropic envelope. This limits transduction to murine cells or human cells manipulated to express the mCAT1 receptor. |
Integrase-deficient lentiviral packaging systemProduce lentivirus that does not integrate into the target-cell genome. |
Lenti-X 293T cell lineNot all 293T cell lines are created equal. This one was clonally selected to produce high lentiviral titers. |
Titration kits
Ten-minute titer testDecide if there is enough lentivirus in your culture supernatant using a simple kit that works like a pregnancy test kit. |
ELISA titration kitMeasure the amount of p24 capsid protein present in your viral supernatant and correlate it directly with lentivirus titer. |
qRT-PCR titration kitUse a fast and simple quantitative RT-PCR kit to measure lentivirus titer. |
Integrated provirus quantitation kitDetermine how many lentiviral genomes have integrated into your target cells (e.g., the copy number). |
Concentration reagent
Lenti-X ConcentratorIncrease lentivirus titer 100X—no ultracentrifugation. Just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge. |
Transduction enhancers
Magnetic transduction beadsPerform lentiviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull lentivirus or retrovirus to your cells with a magnet. |
RetroNectin reagentCoat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes lentivirus (or retrovirus) particles with your target cells. Perfect for hard-to-transduce suspension cells and stem cells. |
Ecotropic Receptor BoosterEfficiently transduce human cells with ecotropic lentivirus or retrovirus that otherwise could only infect rodent cells. The booster consists of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein, the target for ecotropic viruses. |
Purification kit
Lenti-X Maxi Purification KitSimple—just like plasmid maxipreps. A gentle gravity flow-based purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of up to 80%. |
