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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Mammalian Cell ¿ë Vector > ¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors

¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631630
pBI-CMV1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
1,067,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33278
Clontech
631631
pBI-CMV2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,094,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33279
Clontech
631632
pBI-CMV3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,094,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33280
Clontech
631633
pBI-CMV4 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,094,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33281

  • ÇϳªÀÇ ¸ñÀû ´Ü¹éÁú°ú ÇϳªÀÇ reporter ´Ü¹éÁúÀ» Ç×½ÃÀûÀ¸·Î ¹ßÇö
  • ³ì»öÀ̳ª Àû»ö ¶Ç´Â chemiluminescent reporter ¼±Åà °¡´É
Á¦Ç° ¼³¸í
Bidirectional vector´Â ºñ½ÁÇÑ ¼öÁØÀ¸·Î ¸ñÀû ´Ü¹éÁú°ú reporter ´Ü¹éÁú (¶Ç´Â 2 °³ÀÇ ¸ñÀû ´Ü¹éÁú)À» Ç×½ÃÀûÀ¸·Î ¹ßÇöÇÒ ¼ö ÀÖµµ·Ï Á¦À۵Ǿú´Ù. ¸ñÀû ´Ü¹éÁúÀÇ Ç×½ÃÀû ¹ßÇö½Ã½ºÅÛÀ» À§ÇÑ vector´Â ¾Æ·¡¿¡¼­ ¸ñÀû¿¡ ¸Â°Ô ¼±Åà °¡´ÉÇÏ´Ù.
* °¢ vector¿¡ »ðÀԵǴ ¸ñÀû À¯ÀüÀÚ´Â start codon(initiation codon)°ú stop codonÀ» Æ÷ÇÔÇØ¾ß ÇÑ´Ù.

Á¦Ç°¸í

¹ßÇö ´Ü¹éÁú

Vector ±¸Á¶

pBI-CMV1 Vector

2 °³ÀÇ ¸ñÀû ´Ü¹éÁú

pBI-CMV2 Vector

Monomeric GFP AcGFP1

pBI-CMV3 Vector

¾ÆÁÖ ¹àÀº GFP ZsGreen1

pBI-CMV4 Vector

RFP DsRed2

±¸¼º ¹× º¸°ü
- ÀÚ¼¼ÇÑ ±¸¼º°ú º¸°ü Á¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
- Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477-483.
- Jang, S. K. et al. (1988) J. Virol. 62:2636-2643.

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