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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Mammalian Cell ¿ë Vector > EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)

EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631973
pEF1¥á-AcGFP1-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33348
Clontech
631974
pEF1¥á-AcGFP1-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33347
Clontech
631978
pEF1¥á-DsRed-Monomer-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33351
Clontech
631977
pEF1¥á-DsRed-Monomer-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33350
Clontech
631979
pEF1¥á-DsRed-Express2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33349
Clontech
631981
pEF1¥á-E2-Crimson Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33352
Clontech
631969
pEF1¥á-mCherry-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33359
Clontech
631972
pEF1¥á-mCherry-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33358
Clontech
631975
pEF1¥á-tdTomato Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33361
Clontech
631970
pEF1¥á-IRES Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
1,060,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33354
Clontech
631992
pEF1¥á-HA Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
901,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33353
Clontech
631991
pEF1¥á-Myc Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
901,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33360
Clontech
631971
pEF1¥á-IRES-AcGFP1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33355
Clontech
631980
pEF1¥á-IRES-DsRed-Express2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,110,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33356
Clontech
631976
pEF1¥á-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
1,060,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33357

EF-1 Alpha Promoter Vectors
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts (1). EF-1 alpha is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells; 2).
EF-1 alpha promoter vectors are available in multiple formats for either plasmid or lentiviral delivery:
  • Fluorescent protein fusion vectors
  • IRES vectors for coexpression of 2 genes
  • Vectors with highly immunoreactive Myc- or HA-tags that can be used to confirm Matchmaker interactions in mammalian cells

Figure 1. EF-1 alpha expression vectors for many applications. Plasmid and lentiviral vector choices are available carrying the EF-1 alpha promoter. IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site
Storage Conditions
-20¡ÆC
References
1.Teschendorf, C., et al. (2002) Anticancer Res. 22(6A):3325-3330. 2.Kim S, et al. (2007) Stem Cells Dev. 16(4):537-45. 3.Wang, et al. (2008) Stem Cells Dev. 17:279-289.

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