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Gene transfer (virus)
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Mammalian Cell ¿ë Vector
> EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
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Clontech
631973
pEF1¥á-AcGFP1-N1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631974
pEF1¥á-AcGFP1-C1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631978
pEF1¥á-DsRed-Monomer-N1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631977
pEF1¥á-DsRed-Monomer-C1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631979
pEF1¥á-DsRed-Express2 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631981
pEF1¥á-E2-Crimson Vector
10 ¥ìg
1,110,000¿ø
Clontech
631969
pEF1¥á-mCherry-N1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631972
pEF1¥á-mCherry-C1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631975
pEF1¥á-tdTomato Vector
10 ¥ìg
1,110,000¿ø
Clontech
631970
pEF1¥á-IRES Vector
10 ¥ìg
1,060,000¿ø
Clontech
631992
pEF1¥á-HA Vector
10 ¥ìg
901,000¿ø
Clontech
631991
pEF1¥á-Myc Vector
10 ¥ìg
901,000¿ø
Clontech
631971
pEF1¥á-IRES-AcGFP1 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631980
pEF1¥á-IRES-DsRed-Express2 Vector
10 ¥ìg
1,110,000¿ø
Clontech
631976
pEF1¥á-IRES-ZsGreen1 Vector
10 ¥ìg
1,060,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
EF-1 Alpha Promoter Vectors
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts (1). EF-1 alpha is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells; 2).
EF-1 alpha promoter vectors are available in multiple formats for either plasmid or lentiviral delivery:
Fluorescent protein fusion vectors
IRES vectors for coexpression of 2 genes
Vectors with highly immunoreactive Myc- or HA-tags that can be used to confirm Matchmaker interactions in mammalian cells
Figure 1. EF-1 alpha expression vectors for many applications. Plasmid and lentiviral vector choices are available carrying the EF-1 alpha promoter. IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site
Storage Conditions
-20¡ÆC
References
1.Teschendorf, C., et al. (2002) Anticancer Res. 22(6A):3325-3330. 2.Kim S, et al. (2007) Stem Cells Dev. 16(4):537-45. 3.Wang, et al. (2008) Stem Cells Dev. 17:279-289.
Keyword :
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
epitope tag (HA or c-Myc) Vectors
in vivo Vector, pLIVE Vector ½Ã¸®Áî
Magnetic Myc IP Kit
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-CMVvector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
pIRES Bicistronic Expression Vector