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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
4½Ã°£ ¸¸¿¡ LentivirusÀÇ ¿ª°¡¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Clontech
631235
Lenti-X¢â qRT-PCR Titration Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
823,200¿ø 
1,029,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32781, x32782, x32864
Clontech
632200
Lenti-X¢â p24 Rapid Titer Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
932,800¿ø 
1,166,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32776, x32777


  • Á¤È®ÇÑ Lentivirus ¿ª°¡ ÃøÁ¤À¸·Î ¸Å¿ì Àϰü¼ºÀÖ´Â Transduction (¼¼Æ÷°¨¿°) °¡´É
  • HIV-1 ±â¹ÝÀÇ Lentivirus ¿ª°¡¸¦ ½Å¼ÓÇϰí Á¤È®ÇÏ°Ô Á¤·®
  • 4½Ã°£ ¸¸¿¡ Á¤È®ÇÏ°Ô Lentivirus genome copy¸¦ ÃøÁ¤ÇÏ´Â qRT-PCR Kit
  • HIV-1 p24 capsid proteinÀ» ELISA ¹æ½ÄÀ¸·Î ¿ª°¡ ÃøÁ¤ÇÏ´Â p24 Rapid Titer Kit


Virus ¿ª°¡ ÃøÁ¤ÀÌ ÇÊ¿äÇÑ ÀÌÀ¯
    1. Lentivirus Packaging ¹ÝÀÀÀÌ Á¦´ë·Î µÇ¾ú´ÂÁö È®ÀÎ
    2. ¿ÀÂ÷¾øÀÌ ÀϰüµÈ ½ÇÇè°á°ú
    3. 100% TransductionÀ» À§ÇÑ ÃæºÐÇÑ MOI °áÁ¤
    4. Copy number Á¶ÀýÀ» ÅëÇÑ ´Ü¹éÁú ¹ßÇö¾ç Á¶Àý

Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
Lenti-X¢â qRT-PCR Titration Kit´Â °£´ÜÇÏ°í ºü¸¥ Real Time PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© Lentivirus ¿ª°¡¸¦ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº Real Time PCRÀ» ÀÌ¿ëÇÏ¿© Lentiviral genome copy¸¦ ÃøÁ¤ÇÏ´Â °ÍÀº ¹°·Ð, ½Å¼ÓÇÑ RNA Á¤Á¦ °úÁ¤À» Æ÷ÇÔÇϰí ÀÖ´Ù.

Ư¡ 1. Rapid determination of lentiviral titer
ÀÌÀü¿¡ »ç¿ëµÇ¾î¿Ô´ø Lentivirus ¿ª°¡ ÃøÁ¤ ¹æ¹ýÀÌ ¾à 10ÀÏ Á¤µµ°¡ ¼Ò¿äµÇ¾ú´ø ¹Ý¸é, Real Time PCRÀ» »ç¿ëÇÏ´Â º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¸ðµç HIV-1 ±â¹ÝÀÇ Lentivirus vector¸¦ ¾à 4½Ã°£ ¸¸¿¡ Á¤·®ÇÒ ¼ö ÀÖ´Ù. qRT-PCR ¹æ¹ýÀº ¹ÙÀÌ·¯½º ȸ¼ö (virus harvest)¿Í ¸ñÀû¼¼Æ÷ °¨¿° (target cell infection) ±îÁöÀÇ ½Ã°£À» ȹ±âÀûÀ¸·Î °¨¼Ò½Ãų ¼ö ÀÖ¾î, ¹ÙÀÌ·¯½º ³óÃà, ¿ª°¡ ÃøÁ¤, °¨¿°±îÁö ÇÏ·ç¿¡ ¿Ï·áÇÒ ¼ö ÀÖ´Ù.

Ư¡ 2. Achieve consistent lentiviral transductions
Lentiviral gene-deliveryÀÇ ÁÖ¿äÇÑ ÀåÁ¡Àº ¸ñÀû¼¼Æ÷¿¡ µµÀԵǴ viral genome number¸¦ ¿¹Ãø°¡´ÉÇÑ Multiplicity of infection (MOI)¸¦ ¿¹ÃøÇÔÀ¸·Î½á À¯ÀüÀÚ ¹ßÇöÀ» Á¶Àý °¡´ÉÇÏ´Ù´Â Á¡ÀÌ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé Lentivirus gene copy number¿Í lentiviral infectivity »çÀÌÀÇ »ó°ü°ü°è¸¦ ºü¸£°Ô °áÁ¤ÇÒ ¼ö Àֱ⠶§¹®¿¡, ½ÇÇè°£ÀÇ ¸ñÀû À¯ÀüÀÚ ¹ßÇöÀ» º¸´Ù ÀϰüµÇ°Ô Á¶Àý °¡´ÉÇÏ´Ù.

* º» Á¦Ç° ³»¿¡ Æ÷ÇÔµÈ primer´Â Packaging Signal¿¡ ÀÎÁ¢ÇÑ HIV-1 genome ¿µ¿ªÀ» ÁõÆøÇÒ ¼ö ÀÖµµ·Ï µðÀÚÀεǾî ÀÖ½À´Ï´Ù. º» ÇÁ¶óÀ̸Ӵ ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼­ ÆÇ¸ÅÇÏ´Â ¸ðµç Lentivirus vector »Ó¸¸ ¾Æ´Ï¶ó ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â HIV-1 based lentiviral vector¿¡ ȣȯ °¡´ÉÇÕ´Ï´Ù. ¸¸¾à, vector compatibilty¸¦ È®ÀÎÇϰíÀÚ ÇÑ´Ù¸é, °í°´Áö¿ø¼¾ÅÍ (TEL. 02-2081-2510)·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.


±×¸² 1. Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods.
Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.


±×¸² 2. Flowchart of the procedures for titrating lentivirus supernatants using the Lenti-X qRT-PCR Titration Kit.

Lenti-X¢â p24 Rapid Titer Kit (Code 632200)
Lenti-X¢â p24 Rapid Titer Kit´Â ¹ÙÀÌ·¯½º »óÃþ¾× ³»¿¡ Á¸ÀçÇÏ´Â p24 capsid proteinÀÇ ÀÜÁ¸·®À» ÃøÁ¤Çϴ ƯÀ̼º ³ôÀº ELISA¹ýÀ» ÀÌ¿ëÇÑ´Ù. ÀÌ ¶§ ÃøÁ¤ÇÏ´Â p24 capside proteinÀº ¹ÙÀÌ·¯½º ¿ª°¡¿Í Á÷Á¢ÀûÀÎ »ó°ü°ü°è°¡ ÀÖ´Ù. »óÃþ¾×¿¡ Á¸ÀçÇÏ´Â ¹ÙÀÌ·¯½º´Â Á¦Ç° ³» Æ÷ÇÔµÈ lysis buffer·Î ¿ëÇØÇÑ´Ù. ±× ÈÄ, murine anti-p24 antibody°¡ ÄÚÆÃµÈ 96-well microtiter plate¿¡ ¿ëÇØµÈ »ùÇÃÀ» ó¸® ¹× washingÀ» ÁøÇàÇÑ´Ù. ƯÀÌÀûÀ¸·Î °áÇÕµÈ p24 proteinÀ» biotinylated anti-p24 secondary antibody, streptavidine-HRP (horseradish peroxidase) ¹× ¹ß»ö ½Ã¾àÀ¸·Î ¹ÝÀÀ½ÃŲ´Ù. º» Á¦Ç°Àº standard curve¸¦ ÀÛ¼ºÇÒ ¼ö ÀÖ´Â p24 controlÀ» Æ÷ÇÔÇϰí ÀÖ´Ù.


±×¸² 3. Schematic of the Lenti-X p24 Rapid Titer Procedure. p24 from the culture supernatant is lysed and bound to anti-p24 coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a color reagent.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
  • Lenti-X qRT-PCR Titration Components
  • NucleoSpin RNA Virus Kit
  • Quant-X One-Step qRT-PCR TB Green Kit

Lenti-X¢â p24 Rapid Titer Kit (Code 632200)
  • Anti-p24 Coated Plate (96 wells)
  • p24 Control (10ng/ml)
  • Lysis Buffer
  • Anti-p24 (Biotin Conjugate)
  • Streptavidine HRP
  • Wash Buffer (20X Concentrate)
  • TMB Substrate
  • Stop Solution
°ü·ÃÁ¦Ç°
Citation (Lenti-X¢â qRT-PCR Titration Kit)
Temporal plasticity of apical progenitors in the developing mouse neocortex.
Nature | 2020 Mar 17 | PubMed ID: 31462778 | Read Article

A functional variant of SUMO4, a new IκBα modifier, is associated with type 1 diabetes
Nature Genetics | 2004 Jul 11 | PubMed ID: 15247916 | Read Article

Tumor invasion in draining lymph nodes is associated with Treg accumulation in breast cancer patients
Nature Communications | 2020 Jun 29 | PubMed ID: 32601304 | Read Article

Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia
Molecular Neurodegeneration | 2022 Jun 11 | PubMed ID: 35690868 | Read Article

Huaier Extract Inhibits Prostate Cancer Growth via Targeting AR/AR-V7 Pathway
Frontiers in Oncology | 2021 Feb 23 | PubMed ID: 33708629 | Read Article

JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells
Acta Pharmacologica Sinica | 2015 Aug 03 | PubMed ID: 26235742 | Read Article

In Vitro Exposure of Leukocytes to HIV Preexposure Prophylaxis Decreases Mitochondrial Function and Alters Gene Expression Profiles
Antimicrobial Agents and Chemotherapy | 2020 Dec 16 | Read Article

Engineered female-specific lethality for control of pest Lepidoptera.
ACS synthetic biology | 2014 Apr 01 | PubMed ID: 23802263 | Read Article

Interleukin-15 receptor-directed immunotoxins atteunuate disease severity in rat adjuvant arthritis
Molecular Immunology | 2010 Apr 01 | PubMed ID: 20188418 | Read Article

Heterogeneous fates of simultaneously-born neurons in the cortical ventricular zone
Scientific Reports | 2022 Apr 11 | PubMed ID: 35411060 | Read Article

Identification and Functional Analysis of MAPKAPK2 in Hyriopsis cumingii
Genes | 2022 Nov 07 | PubMed ID: 36360298 | Read Article

Colony-stimulating factor CSF2 mediates the phenotypic plasticity of small-cell lung cancer by regulating the p-STAT3/MYC pathway
Oncology Reports | 2022 May 17 | PubMed ID: 35583004 | Read Article

Identification and expression analysis of Langerhans cells marker Langerin/CD207 in grasscarp, Ctenopharyngodon idella.
Gene | 2021 Jan 18 | PubMed ID: 33220343 | Read Article

Identification and expression analysis of a fibrinogen alpha chain-like gene in Atlantic salmon (Salmo salar)
Aquaculture Reports | 2022 Feb 01 | Read Article

Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics
BMC Infectious Diseases | 2018 Dec 20 | PubMed ID: 30572833 | Read Article

>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)
Citation (Lenti-X¢â p24 Rapid Titer Kit)
Deep learning improves prediction of CRISPR-Cpf1 guide RNA activity.
Nature biotechnology | 2018 Dec 19 | PubMed ID: 29431740 | Read Article

Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis
Nature medicine | 2016 May 19 | PubMed ID: 26779814 | Read Article

Identification of evolutionarily conserved gene networks mediating neurodegenerative dementia.
Nature medicine | 2019 Apr 08 | PubMed ID: 30510257 | Read Article

Conversion of adult endothelium to immunocompetent haematopoietic stem cells
Nature | 2018 Feb 01 | PubMed ID: 28514438 | Read Article

The NAD + -mediated self-inhibition mechanism of pro-neurodegenerative SARM1.
Nature | 2021 Nov 16 | PubMed ID: 33053563 | Read Article

Non-Neutralizing Antibodies Alter the Course of HIV-1 Infection in Vivo
Cell | 2018 Aug 10 | PubMed ID: 28757252 | Read Article

Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant
Cell | 2021 Apr 20 | PubMed ID: 33991487 | Read Article

Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia
Cell | 2021 Apr 24 | PubMed ID: 33974910 | Read Article

Blockade of the Phagocytic Receptor MerTK on Tumor-Associated Macrophages Enhances P2X7R-Dependent STING Activation by Tumor-Derived cGAMP.
Immunity | 2020 Apr 21 | PubMed ID: 32049051 | Read Article

Mast Cells Are Crucial for Induction of Group 2 Innate Lymphoid Cells and Clearance of Helminth Infections.
Immunity | 2017 Sep 06 |  PubMed ID: 28514691 | Read Article

Inhibition of nuclear PTEN tyrosine phosphorylation enhances glioma radiation sensitivity through attenuated DNA repair
Cancer cell | 2019 Mar 18 | PubMed ID: 30827889 | Read Article

Tracking genome engineering outcome at individual DNA breakpoints
Nature methods | 2011 Aug 01 | PubMed ID: 21743461 | Read Article

Coupling endonucleases with DNA end-processing enzymes to drive gene disruption
Nature methods | 2013 Mar 20 | PubMed ID: 22941364 | Read Article

Astrocytic process plasticity and IKKβ/NF-κB in central control of blood glucose, blood pressure and body weight
Cell metabolism | 2018 May 02 | PubMed ID: 28467927 | Read Article

Astrocytic process plasticity and IKKβ/NF-κB in central control of blood glucose, blood pressure and body weight
Cell metabolism | 2018 May 02 | PubMed ID: 28467927 | Read Article

Astrocytic process plasticity and IKKβ/NF-κB in central control of blood glucose, blood pressure and body weight
Cell metabolism | 2018 May 02 | PubMed ID: 28467927 | Read Article

Reprogramming of T cell-derived small extracellular vesicles using IL2 surface engineering induces potent anti-cancer effects through miRNA delivery). Reprogramming of T cell-derived small extracellular vesicles using IL2 surface engineering induces potent anti-cancer effects through miRNA delivery
Journal of Extracellular Vesicles | 2022 Nov 29 | Read Article

>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)




Keyword : Lentivirus,·»Æ¼¹ÙÀÌ·¯½º,¿ª°¡,¿ª°¡ÃøÁ¤,Titer,Titration,ŸÀÌÅÍ,Á¤·®,¹ÙÀÌ·¯½º Á¤·®,¹ÙÀÌ·¯½º ·»Æ¼,Lenti

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