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Gene transfer (virus)
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Lentivirus
> Lenti-X¢â CRISPR/Cas9 System
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene delivery
Lenti-X¢â CRISPR/Cas9 System
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
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»ç¿ëÀڸŴº¾ó
Clontech
632629
Lenti-X¢â CRISPR/Cas9 System
Each
(Package)
2,211,000¿ø
Clontech
632630
pLVX-hyg-sgRNA1 Vector System
10 Rxns
(Package)
592,000¿ø
Clontech
632633
Lenti-X¢â Tet-On¢ç 3G CRISPR/Cas9 System
1 System
(Package)
3,562,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Cas9 gene°ú sgRNA¸¦ Lentivirus¸¦ ÀÌ¿ëÇÏ¿© ¼¼Æ÷ ³» µµÀÔ
TransfectionÀÌ ¾î·Á¿î mammalian ¼¼Æ÷ (proliferating and non-proliferating cells)¿¡¼ÀÇ CRISPR/Cas9 Genome editing
Lenti-X¢â Packaging Single shotsÀ» ÀÌ¿ëÇÏ¿© °£´ÜÇÏ°Ô °í¿ª°¡ÀÇ Lentivirus Á¦ÀÛ
Lenti-X¢â Tet-On 3G CRISPR/Cas9 systemÀº Cas9 ¹ßÇöÀ» È¿À²ÀûÀ¸·Î Á¶ÀýÀÌ °¡´ÉÇϸç, ¼¼Æ÷ µ¶¼º ¹®Á¦³ª Cas9ÀÇ Áö¼ÓÀûÀÎ ¹ßÇöÀ¸·Î ÀÎÇÑ off-target effect °¨¼Ò¿¡ È¿°úÀû
Á¦Ç°¼³¸í
Lenti-X¢â CRISPR/Cas9 SystemÀº lentiviral-mediated CRISPR/Cas9 genome editingÀ» À§ÇÑ ¸ðµç ±¸¼ºÇ°ÀÌ Æ÷ÇÔµÈ complete systemÀÌ´Ù.
º» Á¦Ç°Àº °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ´Â Lenti-X¢â Packaging Single Shots°ú Cas9 ´Ü¹éÁú, sgRNA¸¦ ¹ßÇöÇÏ´Â µÎ °³ÀÇ Çö󽺹̵å·Î ±¸¼ºµÇ¾îÀÖ´Ù. ¸ñÀû À¯ÀüÀÚ¸¦ Ÿ°ÙÇÏ´Â sgRNA¸¦ ¹ßÇöÇÏ´Â pLVX-hgy-sgRNA1 plasmid´Â ÀÌ¹Ì ¼±ÇüÈ µÇ¾îÀÖ¾î ¾ÆÁÖ ½±°Ô sgRNA ¼¿À» »ðÀÔ, ¶óÀÌ°ÔÀ̼ÇÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù. Lentivirus¸¦ ÀÌ¿ëÇÏ¿© sgRNA¿Í Cas9 geneÀ» »ðÀÔÇϸé ÀϹÝÀûÀ¸·Î TransfectionÀÌ ¾î·Æ´Ù°í ¾Ë·ÁÁø ¼¼Æ÷ÁÖ¿¡¼ÀÇ CRISPR/Cas9 genome editingÀÌ °¡´ÉÇÏ´Ù (±×¸² 1).
Lenti-X¢â Tet-On 3G CRISPR/Cas9 SystemÀº doxycyclineÀ» ÀÌ¿ëÇÏ¿© Cas9 ¹ßÇöÀ» À¯µµÇÒ ¼ö ÀÖ´Â Tet-on 3G inducible expression Á¦Ç°À¸·Î½á, doxycycline ó¸® ½Ã Cas9 ´Ü¹éÁúÀ» ¹ßÇöÇÑ´Ù. Cas9 ¹ßÇöÀ» ¾ö°ÝÇÏ°Ô Á¶ÀýÇÒ ¼ö Àֱ⠶§¹®¿¡ ¿µ±¸ÀûÀÎ Cas9 ´Ü¹éÁú ¹ßÇöÀ¸·Î ÀÎÇÑ off-target effect³ª ¼¼Æ÷ µ¶¼ºÀ» ÁÙÀÏ ¼ö ÀÖ´Ù (±×¸² 2, ±×¸² 3).
±×¸² 1. Either Jurkat or HT1080 cells were first transduced with LVX-hyg-CD81-sgRNA and then selected for stable integration using hygromycin.
Stable clones were then transduced with LVX-puro-Cas9. Stable clones were selected for using puromycin and then screened for CD81 knockout efficiency using FACS. Positive and negative controls of parental cells without Cas9 transduction were done either with or without anti-CD81 antibody.
±×¸² 2. Inducible knockout of
CD81
in HEK293 cells.
Target cells were first transduced with Tet-On 3G lentivirus and selected with G418. Cas9 induction was then assayed by Western blot using
polyclonal anti-Cas9 antibody (Code 632607)
, diluted 1:1,000, and ECL reagent.
Panel A.
Western blot results for six independent Tet-On 3G-Cas9-positive cell populations cultured in the absence (-) or presence (+) of 0.5 ¥ìg/ml doxycycline, assayed with anti-Cas9 antibody.
Panel B.
FACS results for three independent Tet-On 3G-Cas9-positive 293T cell populations transduced with CD81-sgRNA lentivirus and cultured in the absence (-Dox) or presence (+Dox) of 0.5 ¥ìg/ml doxycycline.
±×¸² 3. Inducible editing of
AAVS1
in HEK293 cells.
Induction of Cas9 expression in six stable Tet-On 3G-Cas9-positive HEK293 cell lines was assayed by qRT-PCR. The
Guide-it Mutation Detection Kit (Code 631443)
was then used to screen for editing of
AAVS1
.
Panel A.
qRT-PCR results for Cas9 expression in uninduced (-Dox) and induced (+Dox) cells for each clone.
Panel B.
Results of Guide-it resolvase assay for detection of
AAVS1
gene editing in uninduced (?) and induced (+) clones.
Applications
Lentivirus-based delivery of a user-defined sgRNA and Cas9 for mammalian genome editing using CRISPR/Cas9 technology
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Lenti-X CRISPR/Cas9 System (Code 632629)
pLVX-hyg-sgRNA1 Vector (Linear) (
Vector map
/
Full sequence (txt.)
)
pLVX-puro-Cas9 Vector (
Vector map
/
Full sequence (txt.)
)
Stellar Competent Cells
Guide-it Ligation Components v2
Lenti-X Packaging Single Shots
pLVX-hyg-sgRNA1 Vector System (Code 632630)
pLVX-hyg-sgRNA1 Vector (
Vector map
/
Full sequence (txt.)
)
Guide-it Ligation Components v2
Stellar Competent Cells
Lenti-X
¢â Tet-On 3G CRISPR/Cas9 System (Code 632633)
pLVX-hyg-sgRNA1 Vector (Linear) (
Vector map
/
Full sequence (txt.)
)
pLVX-TRE3G-Cas9-puro Vector Set
- pLVX-TRE3G-Cas9-puro Vector (
Vector map
/
Full sequence (txt.)
)
- pLVX-TRE3G-Luc Control Vector (
Vector map
/
Full sequence (txt.)
)
pLVX-EF1a-Tet3G Vector (
Vector map
/
Full sequence (txt.)
)
Stellar Competent Cells
Guide-it Ligation Components v2
Lenti-X Packaging Single Shots
Keyword : À¯ÀüÀÚÆíÁý,CRISPR/Cas9,CRISPR,Cas9,gene editing,gene edit,mutation,Knockout,Knock outKO,Lentivirus,Lentivirus CRISPR,·»Æ¼¹ÙÀÌ·¯½º
Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System