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Retro-X Q vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631516
Retro-X Q Vector Set
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg¡¿4
1,180,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33528, x33630, x33005
Clontech
631514
pQCXIN Retroviral Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
858,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33528, x33005
Clontech
631515
pQCXIX Retroviral Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
858,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33529, x33005
Clontech
632505
pRetroQ-AcGFP1-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33532, x33005
Clontech
632506
pRetroQ-AcGFP1-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33531, x33005
Clontech
632507
pRetroQ-DsRed-Monomer-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33534, x33005
Clontech
632508
pRetroQ-DsRed-Monomer-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33533, x33005
Clontech
632567
pRetroQ-mCherry-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33535, x33005
Clontech
632568
pRetroQ-mCherry-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33536, x33005

  • 5£§ LTRÀÇ promoter·Î ´Ù¸¥ retroivirus vectorº¸´Ù ³ôÀº ¿ª°¡¸¦ »ý»ê
  • Promoter interference Á¦°Å·Î ½Å·Ú¼º ³ôÀº ¹ßÇö°ú bicistronic ¹ßÇö
  • Çü±¤ ¸¶Ä¿¸¦ Æ÷ÇÔÇÏ¿© ´Ù¼öÀÇ ¼±º° ¸¶Ä¿
Retro-X Q Vector´Â °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º »ý»ê°ú ½Å·Ú¼º ³ôÀº ¹ßÇöÀ» ½ÇÇöÇÏ°í, promoter interferenceÀÇ °¡´É¼ºÀ» ÃÖ¼ÒÈ­ Çϵµ·Ï ¼³°èµÇ¾ú´Ù (1).
º» Q Vector´Â Ç×»ýÁ¦ ¶Ç´Â Çü±¤ ¼±º° ¸¶Ä¿ (Figure 1)¿Í ÇÔ²² ¸ñÀû À¯ÀüÀÚ°¡ ¹ßÇöµÇ°í, ¼÷ÁÖÀÇ genomeÀ¸·Î »ðÀÔµÈ ÈÄ 5£§LTRÀÇ promoter°¡ ºñÈ°¼ºµÇµµ·Ï ¼³°èµÇ¾ú´Ù (Figure 2). Self-inactivating vector´Â promoter interference (2-5)ÀÇ °¡´É¼ºÀ» °¨¼Ò½ÃÄÑ ¹ßÇöÀ» Çâ»ó½ÃŲ´Ù. MMLV LTR¿¡¼­ transcript¸¦ È¿°úÀûÀ¸·Î ¹ßÇöÇÏÁö ¸øÇÏ´Â ¼¼Æ÷¿¡¼­µµ È®½ÇÇÑ ¹ßÇöÀ» º¸¿©ÁØ´Ù(6).


Figure 1. Composite Retro-X Q Vector Set map. In this generalized version of the Q Vector Map, the common elements of all the vectors are represented. Immediately downstream of the CMV immediate early promoter, the multiple cloning site (MCS) is followed by a eukaryotic IRES that ensures a second ORF (an antibiotic resistance marker or another gene in the case of pQCXIX) is cotranscribed with the gene cloned into the MCS. The expression cassette has all of the essential elements for retroviral integration and expression.
Self-Inactivation Vector·Î ³ôÀº ¹ÙÀÌ·¯½º ¿ª°¡ »ý»ê.
5£§LTRÀÇ CMV/MSV hybrid promoter´Â packaging °úÁ¤ µ¿¾È ³ôÀº ¿ª°¡¸¦ À¯µµÇÑ´Ù. ±×·± ´ÙÀ½ ¼÷ÁÖ genomeÀ¸·Î »ðÀԵǴ µ¿¾È 3£§LTRÀÇ U3 ¿µ¿ªÀÇ °á½ÇÀÌ 5£§LTR¿¡ º¹Á¦µÇ¾î ºñÈ°¼ºÈ­½ÃŲ´Ù. µû¶ó¼­ MCSÀÇ ¹Ù·Î »ó·ù¿¡ À§Ä¡ÇÑ ³»ºÎ CMV promoter¿¡ ÀÇÇؼ­¸¸ transcript°¡ ¹ßÇöµÈ´Ù. Vector set´Â 3°³ÀÇ Ç×»ýÁ¦ ¼±º° ¸¶Ä¿ (Hyg, Pur, Neo)¸¦ °¡Áö°í ÀÖÀ¸¸ç, À̵éÀº ¸ñÀû À¯ÀüÀÚ¿Í ÇÔ²² internal ribosome entry site (IRES)¸¦ ÅëÇÏ¿© bicistron Çü½ÄÀ¸·Î ¹ßÇöµÈ´Ù. LacZ control vector (pQCLIN) ¶ÇÇÑ ¼¼Æ®¿¡ Æ÷ÇԵǾî ÀÖ´Ù.


Figure 2. Self-inactivation mechanism of the Retro-X Q Vectors. Plus strand viral RNA (blue) from the expression cassette is reverse transcribed. During integration, a circular intermediate is formed that results in duplication of the deletion in the U3 region of the 3' LTR. This inactivates the CMV/MSV hybrid promoter in the 5' LTR so that transcription can only be driven from the internal promoter, PCMV IE (Figure 1).
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Julius, M. A. et al. (2000) BioTechniques 28:702-708.
2. Emerman, M. & Temin, H. M. (1984) Cell 39:449-467.
3. Yee, J. K. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5197-5201.
4. Nakajima, K. et al. (1993) FEBS Lett. 315:129-133.
5. Zufferey, R. et al. (1998) J. Virol. 72:9873-9880.
6. Soriano, P. et al. (1991) J. Virol. 65:2314-2319.

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