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Gene transfer (virus)
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Retrovirus
> Titer Set (for Real Time PCR)
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ÃøÁ¤
Titer Set (for Real Time PCR)
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Takara
6166
Retrovirus Titer Set (for Real Time PCR)
100 ¹ÝÀÀºÐ
348,800¿ø
436,000¿ø
09.02 ~ 10.31
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
³»¿ë (100 ¹ÝÀÀ ºÐ ; 25§¡ ¹ÝÀÀ°è
*1
)
Forward Titer Primer FRT-1 (10 pmol/§¡)
50 §¡
Reverse Titer primer RRT-1 (10 pmol/§¡)
50 §¡
RNA Control Template (5 ¡¿ 10
8
copies/§¡)
10 §¡
EASY Dilution (Real Time PCR ¿ë)
1 §¢ ¡¿ 2
DNase I (RNase Free) (5 U/§¡)
40 §¡
10 ¡¿ DNase I Buffer
50 §¡
RNase Inhibitor (porcine liver) (40 U/§¡)
10 §¡
*1
Real Time RT- PCR 100ȸ ±×¸®°í DNase I ó¸® 20ȸÀÓ.
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RNA Control Template´Â ¹Ýº¹ÀûÀÎ µ¿°á À¶ÇØ¿¡ ÀÇÇÑ ºÐÇظ¦ ¸·±â À§ÇÏ¿© ¼Ò·® ºÐÁÖ ÈÄ Deep freezer (-70¡-80¡É)¿¡¼ ÀúÀåÇÏ´Â °ÍÀ» ÃßõÇÕ´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Real Time RT-PCRÀ» ÀÌ¿ëÇÏ¿© MLV (murine leukemia virus) À¯·¡ retrovirus vectorÀÇ RNA genome ·®À» ÃøÁ¤ÇÏ¿© ¿ª°¡(RNA titer)À» »êÃâÇÏ´Â Á¦Ç°ÀÌ´Ù.
º» Á¦Ç°À» One Step TB Green¢â PrimeScript RT-PCR Kit (Perfect Real Time) (Code RR066A/RR086A)°ú ÇÔ²² »ç¿ëÇÏ¿© ¹ÙÀÌ·¯½º »óµî¾×¿¡¼ Á÷Á¢ RNA titer¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ°í, ¾à 4½Ã°£ ¾È¿¡ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ¶ÇÇÑ, MLVÀÇ packaging signalºÎºÐÀ» ÁõÆø ´ë»óÀ¸·Î ÇÏ°í ÀÖ¾î °ÅÀÇ ¸ðµç MLV À¯·¡ RetrovirusÀÇ ¿ª°¡(RNA titer)¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù.
RetrovirusÀÇ °¨¿° ¿ª°¡¸¦ ÃøÁ¤ÇÒ °æ¿ì ÀϹÝÀûÀ¸·Î Ç¥Àû ¼¼Æ÷¿¡ Retrovirus¸¦ °¨¿°½ÃÅ°°í Çü±¤À̳ª ¾àÁ¦³»¼º µîÀ» ÀÌ¿ëÇÏ¿© °¨¿°È¿À²À» ÃøÁ¤ÇØ ¿ª°¡ (»ý¹°ÇÐÀû titer)À» »êÃâÇÏ´Â ¹æ¹ýÀÌ ÀÌ¿ëµÈ´Ù. ÀÌ ¹æ¹ýÀº ¼öÀÏÀÌ ¼Ò¿äµÇ¸ç, ¸¶Ä¿À¯ÀüÀÚ³ª ¾àÁ¦³»¼ºÀ¯ÀüÀÚ°¡ Æ÷ÇÔµÇÁö ¾ÊÀº Retrovirus vector¿¡´Â Àû¿ëÇÒ ¼ö ¾ø´Ù. ¶ÇÇÑ »ý¹°ÇÐÀû titer¸¦ ±âÁØÀ¸·Î MOI (multiplicity of infection)¸¦ ÃøÁ¤ÇØ ¼¼Æ÷¿¡ °¨¿°½Ãų °æ¿ì, ¿ª°¡ ÃøÁ¤¿¡ ¸çÄ¥ÀÌ ¼Ò¿äµÇ¹Ç·Î ÀÏ´Ü ¹ÙÀÌ·¯½º »óµî¾×À» µ¿°á º¸Á¸ÇÒ ÇÊ¿ä°¡ ÀÖ°í, µ¿°á À¶ÇØ¿¡ ÀÇÇØ ¹ÙÀÌ·¯½º ¿ª°¡°¡ ÀúÇ쵃 °¡´É¼ºÀÌ ÀÖÀ¸¹Ç·Î Ãʱâ ÃøÁ¤°ª°ú´Â ´Ù¸¥ MOIÀÇ ¹ÙÀÌ·¯½º°¡ ½ÇÇè¿¡ ÀÌ¿ëµÉ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº RetrovirusÀÇ RNA genome·®À» ÃøÁ¤Çϱ⠶§¹®¿¡ ¸¶Ä¿À¯ÀüÀÚ³ª ¾àÁ¦³»¼ºÀ¯ÀüÀÚ¿Í´Â °ü°è¾øÀÌ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¾à 4½Ã°£ ¸¸¿¡ ÃøÁ¤ °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ¸¹Ç·Î ¹ÙÀÌ·¯½º »óµî¾×À» µ¿°áÇÏÁö ¾Ê°í ¿øÇÏ´Â MOI·Î °¨¿°½Ãų ¼ö ÀÖ´Ù. ÇÔ²² »ç¿ëÇÏ´Â One Step TB Green¢â PrimeScript RTPCR Kit (Perfect Real Time) Àº TB Green¢â °ËÃâ¿¡ ÀÇÇÑ 1 step Real Time RT-PCR Å°Æ®ÀÌ´Ù. ½ÅÀ强ÀÌ ¶Ù¾î³ª ´Ü½Ã°£¿¡ È¿À²ÀûÀ¸·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÑ ¿ªÀü»ç È¿¼Ò PrimeScript ¿Í ´ÙÄ«¶óÀÇ ´ëÇ¥ÀûÀÎ PCR È¿¼ÒÀÎ
TaKaRa Ex Taq
HS¸¦ ÀÌ¿ëÇÏ¿© 1 step RT-PCR¿¡ ÃÖÀûÈÇÏ°í ÀÖÀ¸¸ç ¾ÈÁ¤ÀûÀ¸·Î 1 step real time RT-PCRÀ» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ ½Ã¾à
One Step TB Green¢â PrimeScript RT-PCR Kit (Perfect Real Time) (TaKaRa Code RR066A)
¶Ç´Â One Step TB Green¢â PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa Code RR086A)
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit