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Gene transfer (virus)
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Adenovirus
> qPCR titer ÃøÁ¤ Kit
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô ÃøÁ¤
qPCR titer ÃøÁ¤ Kit
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Clontech
632252
Adeno-X qPCR Titration Kit
200 ȸ
(Package)
978,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
ÀçÁ¶ÇÕ adenovirusÀÇ ¿ª°¡ ÃøÁ¤À» À§ÇÑ ±â´ÉÀûÀÎ qPCR ½ÇÇè¹ý
4 ½Ã°£ À̳»·Î ½Å¼ÓÇÏ°Ô ¹ÙÀÌ·¯½º ¿ª°¡ ÃøÁ¤
Plaque assay ¶Ç´Â End-point dilution assayº¸´Ù ´õ ºü¸£°í Á¤È®
Adeno-X qPCR Titration Kit
Adeno-X qPCR Titration Kit´Â Adeno-X Expression SystemÀÇ Ad5-based adenovirus vector·ÎºÎÅÍ »ý»êµÈ adenovirusÀÇ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Â ¸Å¿ì ½Å¼ÓÇÏ°í Á¤È®ÇÑ ¹æ¹ýÀÌ´Ù (1). Á¶ÀÛ °úÁ¤Àº Intercalater ¹æ¹ýÀ¸·Î DNA Ç¥ÁØ °î¼±À» ÀÌ¿ëÇÏ¿© ÁغñµÈ ¹ÙÀÌ·¯½º (crude lysates ¶Ç´Â purified stock)ÀÇ genome copy ¼ö¸¦ ÃøÁ¤ÇÑ´Ù (Figure 1). º» Á¦Ç°Àº ´Ü 4½Ã°£ ¸¸¿¡ °á°ú¸¦ ¾òÀ» ¼ö Àֱ⠶§¹®¿¡ ´çÀÏ ¹ÙÀÌ·¯½º¸¦ ³óÃà°ú µ¿½Ã¿¡ Á¤È®ÇÏ°Ô ¹ÙÀÌ·¯½º ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿© ¸ñÀû ¼¼Æ÷·Î °¨¿° ½Ãų ¼ö ÀÖ´Ù.
Figure 1. Flowchart of the procedures used for titrating adenoviral DNA with the Adeno-X qPCR Titration Kit.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Obtain Adenoviral Titers in Less than 4 hours (2009) Clontechniques XXIV(2):12-13.
2. Bewig, B. & Schmidt, W. E. (2000) Biotechniques 28(5):870-873.
3. Graham, F. L. et al. (1977) J. Gen. Virol. 36(1):59-74.
Keyword : ¿ª°¡ÃøÁ¤,Titration,Titer,ŸÀÌÅÍ,¿ª°¡,qPCR,¹ÙÀÌ·¯½º¿ª°¡,¹ÙÀÌ·¯½º ¿ª°¡,Virus genome copies,Virus copy,Virus copies,Adenovirus ¿ª°¡
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression System 3
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Mega type)
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
¸é¿ª ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£¿¡ °¨¿° titer ÃøÁ¤
Hexon ±â¹Ý titer ÃøÁ¤
°£ÆíÇÑ ¿ª°¡ ÃæºÐ ¿©ºÎ °ËÃâ Å°Æ®
Adeno-X GoStix
CAR Receptor Booster