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Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, AAV5, AAV6 Á¦ÀÛ

CRE Recombinase System

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6668
AAVpro¢ç Helper Free System(AAV1-CRE Recombinase)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
1,131,200¿ø 
1,414,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6230_6650-6657_6668_6669_6673_e.v1611Da.pdf
Takara
6652
AAVpro¢ç Helper Free System (AAV2-CRE Recombinase)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
1,131,200¿ø 
1,414,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6230_6650-6657_6668_6669_6673_e.v1611Da.pdf
Takara
6653
AAVpro¢ç Helper Free System(AAV5-CRE Recombinase)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
1,131,200¿ø 
1,414,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6230_6650-6657_6668_6669_6673_e.v1611Da.pdf
Takara
6654
AAVpro¢ç Helper Free System(AAV6-CRE Recombinase)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
1,131,200¿ø 
1,414,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6230_6650-6657_6668_6669_6673_e.v1611Da.pdf

  • Helper virus¸¦ »ç¿ëÇÏÁö ¾Ê°í ¾ÈÀüÇÏ°Ô Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, AAV5, AAV6¸¦ Á¦ÀÛ
  • loxP ÀÇÁ¸ÀûÀÎ recombinaseÀÎ Cre À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â AAV¸¦ Á¦ÀÛÇÏ¿© ÇüÁúÀüȯ ¸¶¿ì½º Á¦ÀÛ ¹× °¢Á¾ ½ºÅ©¸®´×¿¡ ³Î¸® Àû¿ë
Á¦Ç° ¼³¸í
Adeno-Associated Virus(AAV)´Â ºÐ¿­Çϰųª ºÐ¿­ÇÏÁö ¾Ê´Â ¼¼Æ÷ ¸ðµÎ¿¡ °¨¿°½Ãų ¼ö ÀÖ´Â non-envelopedÀÇ single stranded DNA virusÀÌ´Ù. AAV´Â helper virus°¡ Á¸Àç½Ã¿¡¸¸ º¹Á¦°¡ °¡´ÉÇϸé Àΰ£¿¡°Ô´Â ºñº´¿ø¼ºÀÌ´Ù. ÀÌ·± Ư¡À¸·Î ´Ù¾çÇÑ ¼¼Æ÷¿¡ À¯ÀüÀÚ¸¦ µµÀÔÇÏ´Â À¯¿ëÇÑ ¹æ¹ýÀ̸ç À¯ÀüÀÚÄ¡·á¸¦ À§ÇÑ À¯¿ëÇÑ vector·Î »ç¿ëµÇ°í ÀÖ´Ù.
AAV´Â ´Ù¾çÇÑ Ç÷ûÇüÀÌ Á¸ÀçÇÏ°í Ç÷ûÇü¿¡ µû¶ó host³ª ¹ÙÀÌ·¯½ºÀÇ Æ¯Â¡ÀÌ ´Ù¸¥ °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. Serotype 2 (AAV2)´Â ¿À·¡ÀüºÎÅÍ ³Î¸® ¿¬±¸µÇ¾î ¿Â serotypeÀ¸·Î ´Ù¾çÇÑ ¼¼Æ÷¸¦ °¨¿°½Ãų ¼ö ÀÖ´Ù. Serotype 1 (AAV1), Serotype 5 (AAV5), Serotype 6 (AAV6)´Â º¸´Ù Á¶Á÷ °¨¿°Æ¯À̼ºÀ» °¡Áø Ç÷ûÇüÀ¸·Î, AAV1Àº ±ÙÀ°, °£, ±âµµ, ÁßÃ߽Űæ°è µî¿¡, AAV5 ´Â ÁßÃ߽Űæ°è, °£, ¸Á¸· µî¿¡, AAV6´Â ½ÉÀå, ±ÙÀ°, °£ µî¿¡ ´ëÇÑ À¯ÀüÀÚ µµÀÔÈ¿À²ÀÌ ³ô´Ù°í ¾Ë·ÁÁ® ÀÖ´Ù.

´ÙÄ«¶ó¿¡¼­´Â Serotype 1, 2, 5, 6ÀÇ AAV vector¸¦ helper virus¾øÀÌ ¾ÈÀüÇÏ°Ô Á¦ÀÛÇϱâ À§ÇÑ AAVpro Helper Free SystemÀ» ÆǸÅÇÏ°í ÀÖÀ¸¸ç, ¸¸µé¾îÁø AAV ¹ÙÀÌ·¯½º ÀÔÀÚ¸¦ ÀÌ¿ëÇÏ¿© Ç¥Àû ¼¼Æ÷³ª µ¿¹° °³Ã¼¿¡¼­ ¸ñÀû À¯ÀüÀÚ¸¦ ÀϽÃÀûÀ¸·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù.

º» Á¦Ç°Àº loxP ÀÇÁ¸ÀûÀÎ recombinaseÀÎ Cre À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, AAV5, AAV6¸¦ helper virus¸¦ »ç¿ëÇÏÁö ¾Ê°í ¾ÈÀüÇÏ°Ô Á¦ÀÛÇϱâ À§ÇÑ Á¦Ç°À¸·Î Á¦ÀÛµÈ AAV-Cre recombinase´Â ÇüÁúÀüȯ ¸¶¿ì½º Á¦ÀÛ°ú °¢Á¾ ½ºÅ©¸®´×¿¡ ³Î¸® »ç¿ëÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. AAV2-Cre¸Å°³Ã¼ÀÇ ¼¼Æ÷ÁÖ¿¡ ´ëÇÑ À¯ÀüÀÚ µµÀÔ
loxP-ZsGÀ» ¹ßÇöÇÏ´Â HEK293¼¼Æ÷¿¡ AAV2-Cre¸¦ °¨¿°½ÃÅ°¸é loxP ÀÇÁ¸ÀûÀÎ º¯ÇüÀÌ ÀϾ stuffer¹è¿­ÀÌ Á¦¿ÜµÊÀ¸·Î½á ÇÏ·ùÀÇ ZsGreenÀÌ ¹ßÇöÇÑ´Ù.


±×¸² 2. AAV2-Cre ¿¡ °¨¿°µÈ HEK293 ¼¼Æ÷ÀÇ FACS ºÐ¼®
AAVpro¢ç Purification Kit (AAV2)(Code 6232)¸¦ ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÑ AAV2-Cre¸¦ loxP-ZsGÀ» ¹ßÇöÇÏ´Â HEK293¼¼Æ÷¿¡ °¨¿°½ÃÄ×À» ¶§, AAV2-Cre °¨¿°·®¿¡ µû¶ó Çü±¤À» ¹ßÇÏ´Â HEK293 ¼¼Æ÷°¡ Áõ°¡ÇÏ¿´´Ù.

AAVpro¢ç Helper Free SystemÀ¸·Î AAV Particle Á¦ÀÛ
AAVpro¢ç Helper Free SystemÀº helper virus¸¦ »ç¿ëÇÏÁö ¾Ê°í, °í¿ª°¡ÀÇ AAV¸¦ Á¦ÀÛÇÏ´Â ½Ã½ºÅÛÀÌ´Ù. º» Á¦Ç°¿¡ Æ÷ÇÔµÈ 3Á¾·ùÀÇ Çö󽺹̵带 AAVpro¢ç 293T Cell Line (Code 632273)¿¡ µµÀÔÇÏ¿© °£ÆíÇÏ°Ô AAVÀÔÀÚ¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.

  • pAAV-CRE Recombinase Vector: Cre-Recombinase À¯ÀüÀÚ¿Í 2°³ÀÇ ITRÀ» Æ÷ÇÔÇÏ´Â vector plasmid
  • pRC Vector: AAV2ÀÇ Rep À¯ÀüÀÚ¿Í °¢ serotypeÀÇ Cap À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏÇÑ packaging plasmid
  • pHelper: AAV¸¦ »ý»êÇϱâ À§ÇØ ÇÊ¿äÇÑ adenovirus E2A, E4¿Í VA À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏ´Â packaging plasmid

±âÁ¸¿¡ µ¿°á/À¶Çعý ¶Ç´Â ÃÊÀ½ÆÄ Æļâ¹ýÀÌ ¾Æ´Ñ ´ÙÄ«¶ó¿¡¼­ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ AAVpro¢ç Extraction SolutionÀ» ÀÌ¿ëÇϸé AAV producing cell·ÎºÎÅÍ ¼÷ÁִܹéÁúÀ̳ª ÇÙ»êÀÇ È¥ÀÔÀ» ÃÖ¼ÒÈ­Çϸç, °£ÆíÇÏ°Ô AAV vectorÀÔÀÚ¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù. ÃßÃâ½Ã¾àÀº System Á¦Ç°³»¿¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, AAVpro¢ç Extraction Solution (Code 6235)À¸·Îµµ ÆǸÅÇÏ°í ÀÖ´Ù.
¶ÇÇÑ °íÈ¿À²ÀÇ in vivo À¯ÀüÀÚ µµÀÔÀ» À§ÇÑ AAV Á¤Á¦¿¡´Â AAVpro¢ç Purification Kit (All Serotype) (Code 6666) ¶Ç´Â AAVpro¢ç Purification Kit (AAV2)(Code 6232)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.


±×¸² 3. AAVpro¢ç Helper Free System¿¡ ÀÇÇÑ AAV Á¦ÀÛ
³»¿ë
AAVpro¢ç Helper Free System (AAV2-CRE Recombinase) (Code 6652)
1

pAAV-CRE Recombinase Vector (1 §¶/§¡)

20 §¡

2

pRC2-mi342 Vector (1 §¶/§¡) *1

20 §¡

3

pHelper Vector (1 §¶/§¡)

20 §¡

4

AAV Extraction Solution A

1.5 §¢ ¡¿ 3

5

AAV Extraction Solution B

150 §¡ ¡¿ 3

*1 AAVpro¢ç Helper Free System (AAV1-CRE Recombinase) (Code 6668)¿¡´Â pRC1 Vector°¡ Æ÷ÇԵǾî ÀÖ´Ù.
*1 AAVpro¢ç Helper Free System (AAV5-CRE Recombinase) (Code 6653)¿¡´Â pRC5 Vector°¡ Æ÷ÇԵǾî ÀÖ´Ù.
*1 AAVpro¢ç Helper Free System (AAV6-CRE Recombinase) (Code 6654)¿¡´Â pRC6 Vector°¡ Æ÷ÇԵǾî ÀÖ´Ù.
º¸Á¸ -20 ¡É
´Ü, AAV Extraction Solution A¹× AAV Extraction Solution B´Â À¶ÇØ ÈÄ, ½Ç¿Â ÀúÀå
°ü·ÃÁ¦Ç°

Keyword : Adeno Associated Virus,in vivo,aav virus

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
>
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
>
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
>
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
>
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
>
Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
>
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
>
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
>
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
>
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
>
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
>
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution