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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)

Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631530
Retro-X Universal Packaging System
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
1 Set
(Package)
1,665,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33346, x33615, x33224, x33265, x33005, x33178
Clontech
632105
Retro-X Tet-Off Advanced Inducible Expression System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
(Package)
1,932,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33549, x33555, x33556, x32537, x33013
Clontech
631034
pRetroX-Tight-Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,023,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33553, x33554, x33005
Clontech
631311
Doxycycline
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 g
376,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x94899, x32770, x32771

  • Çâ»óµÈ Tet-Advanced inducible systemÀÇ È¿À²ÀûÀÎ retroivirus Àü´Þ
  • ´ëºÎºÐÀÇ ºÐ¿­ ¼¼Æ÷ (dividing cell)¿¡ ÈǸ¢ÇÏ°Ô Àû¿ë
  • ¶Ù¾î³­ À¯µµ Á¶Àý°ú ¸Å¿ì ³·Àº basal expression
ClontechÀÇ Retro-X Tet-On Advanced¿Í Retro-X Tet-Off Advanced Inducible Expression SystemsÀº ClontechÀÇ ÃֽŠtetracycline Á¶Àý À¯ÀüÀÚ ¹ßÇö ½Ã½ºÅÛ°ú È¿°úÀûÀ¸·Î À¯ÀüÀÚ¸¦ µµÀÔ½ÃÅ°´Â retroivirus°¡ °áÇÕµÈ Á¦Ç°ÀÌ´Ù (1). µÎ ½Ã½ºÅÛ Áß retroivirus vector´Â Tet-Advanced inducible gene expression system¿¡ ´Ù¿ëµµ·Î »ç¿ëµÉ ¼ö ÀÖÀ¸¸ç, ±¤¹üÀ§ÇÑ primary cell culture¿Í immortalized cell lines¿¡µµ »ç¿ëµÉ ¼ö ÀÖ´Ù. Cell lines¸¦ ¸¸µé¾î ³õÀ¸¸é, DoxÀÇ ³óµµ Á¶ÀýÀ» ÅëÇØ ½Ã½ºÅÛÀÇ È°¼ºÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù (Figure 1).
Tet-Advanced SystemÀº ¸Å¿ì ³ôÀº ƯÀ̼ºÀ¸·Î ³ôÀº ¹ßÇöÀ» ³ªÅ¸³»¸ç, ¿©·¯°¡Áö Çö»óµé¿¡ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù.
Retroivirus Vector¿Í Packaging¿¡ ÀÌ»óÀû
CalPhos Mammalian Transfection Kit (Cat. No. 631312)´Â °¢ ½Ã½ºÅÛ¿¡ Æ÷ÇÔµÇ¾î ¸Å¿ì È¿°úÀûÀÎ packaging cell transfectionÀ» º¸ÁõÇÑ´Ù. º» ½Ã½ºÅÛÀº Retro-X Universal Packaging System (Cat. No. 631530, º°¸Å)°ú ÇÔ²² »ç¿ëÇÒ ¼ö Àֱ⠶§¹®¿¡, ¸ñÀû ¼¼Æ÷¿¡ ¼÷ÁÖ ÁöÇ⼺À» ¸ÂÃá ¹ÙÀÌ·¯½º¸¦ ¸Å¿ì ³ôÀº ¿ª°¡·Î »ý»êÇÑ´Ù. ¶ÇÇÑ, ¸ñÀû retrovirus¸¦ cell line À̳ª primary cell culture¿¡µµ È¿°úÀûÀ¸·Î µµÀÔ ½Ãų ¼ö ÀÖµµ·Ï »ç¿ëÀÚ°¡ Á÷Á¢ ¼÷ÁÖ ÁöÇ⼺À» ¼±ÅÃÇÒ ¼ö ÀÖ´Ù. Vector´Â packaging cell¿¡¼­ °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º¸¦ »ý»êÇÒ ¼ö ÀÖµµ·Ï ÇÏ´Â Retro-X Q ±â¼úÀ» Ư¡À¸·Î ÇÑ´Ù.


Figure 1. High induction with the Retro-X Tet-Off Advanced System. HeLa cells stably transduced with Retro-X Tet-Off Advanced were subsequently infected with a Retro-X-Tight-Pur virus engineered for inducible luciferase expression. Infected cells were grown in the absence (-) or presence (+) of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Inducible Retroviral Gene Expression Systems (July 2007) Clontechniques XXII(3):2-3.

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