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Gene transfer (virus)
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Retrovirus
> Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
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Clontech
631530
Retro-X Universal Packaging System
1 Set
(Package)
1,665,000¿ø
,
,
,
,
,
Clontech
632105
Retro-X Tet-Off Advanced Inducible Expression System
1 Kit
(Package)
1,932,000¿ø
,
,
,
,
Clontech
631034
pRetroX-Tight-Hyg Vector
20 ¥ìg
1,023,000¿ø
,
,
Clontech
631311
Doxycycline
5 g
376,000¿ø
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Çâ»óµÈ Tet-Advanced inducible systemÀÇ È¿À²ÀûÀÎ retroivirus Àü´Þ
´ëºÎºÐÀÇ ºÐ¿ ¼¼Æ÷ (dividing cell)¿¡ ÈǸ¢ÇÏ°Ô Àû¿ë
¶Ù¾î³ À¯µµ Á¶Àý°ú ¸Å¿ì ³·Àº basal expression
ClontechÀÇ Retro-X Tet-On Advanced¿Í Retro-X Tet-Off Advanced Inducible Expression SystemsÀº ClontechÀÇ ÃֽŠtetracycline Á¶Àý À¯ÀüÀÚ ¹ßÇö ½Ã½ºÅÛ°ú È¿°úÀûÀ¸·Î À¯ÀüÀÚ¸¦ µµÀÔ½ÃÅ°´Â retroivirus°¡ °áÇÕµÈ Á¦Ç°ÀÌ´Ù (1). µÎ ½Ã½ºÅÛ Áß retroivirus vector´Â Tet-Advanced inducible gene expression system¿¡ ´Ù¿ëµµ·Î »ç¿ëµÉ ¼ö ÀÖÀ¸¸ç, ±¤¹üÀ§ÇÑ primary cell culture¿Í immortalized cell lines¿¡µµ »ç¿ëµÉ ¼ö ÀÖ´Ù. Cell lines¸¦ ¸¸µé¾î ³õÀ¸¸é, DoxÀÇ ³óµµ Á¶ÀýÀ» ÅëÇØ ½Ã½ºÅÛÀÇ È°¼ºÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù (Figure 1).
Tet-Advanced SystemÀº ¸Å¿ì ³ôÀº ƯÀ̼ºÀ¸·Î ³ôÀº ¹ßÇöÀ» ³ªÅ¸³»¸ç, ¿©·¯°¡Áö Çö»óµé¿¡ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù.
Retroivirus Vector¿Í Packaging¿¡ ÀÌ»óÀû
CalPhos Mammalian Transfection Kit (Cat. No. 631312)´Â °¢ ½Ã½ºÅÛ¿¡ Æ÷ÇÔµÇ¾î ¸Å¿ì È¿°úÀûÀÎ packaging cell transfectionÀ» º¸ÁõÇÑ´Ù. º» ½Ã½ºÅÛÀº Retro-X Universal Packaging System (Cat. No. 631530, º°¸Å)°ú ÇÔ²² »ç¿ëÇÒ ¼ö Àֱ⠶§¹®¿¡, ¸ñÀû ¼¼Æ÷¿¡ ¼÷ÁÖ ÁöÇ⼺À» ¸ÂÃá ¹ÙÀÌ·¯½º¸¦ ¸Å¿ì ³ôÀº ¿ª°¡·Î »ý»êÇÑ´Ù. ¶ÇÇÑ, ¸ñÀû retrovirus¸¦ cell line À̳ª primary cell culture¿¡µµ È¿°úÀûÀ¸·Î µµÀÔ ½Ãų ¼ö ÀÖµµ·Ï »ç¿ëÀÚ°¡ Á÷Á¢ ¼÷ÁÖ ÁöÇ⼺À» ¼±ÅÃÇÒ ¼ö ÀÖ´Ù. Vector´Â packaging cell¿¡¼ °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º¸¦ »ý»êÇÒ ¼ö ÀÖµµ·Ï ÇÏ´Â Retro-X Q ±â¼úÀ» Ư¡À¸·Î ÇÑ´Ù.
Figure 1. High induction with the Retro-X Tet-Off Advanced System.
HeLa cells stably transduced with Retro-X Tet-Off Advanced were subsequently infected with a Retro-X-Tight-Pur virus engineered for inducible luciferase expression. Infected cells were grown in the absence (-) or presence (+) of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Inducible Retroviral Gene Expression Systems (July 2007) Clontechniques XXII(3):2-3.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit