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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Adeno-Associated Virus (AAV) > AAV Freeze-thaw extraction solution
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AAV Freeze-thaw extraction solution

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Takara
6679
AAVpro¢ç Freeze-Thaw Extraction Buffer (All Serotypes)
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20 §¢ *2
(Package)
196,800¿ø 
246,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6679_e.v2108Da.pdf
Takara
6678
AAVpro¢ç Purification Pack Maxi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱâ
4ȸ
(Package)
580,000¿ø 
725,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6676-6678_e.v2108Da.pdf

  • ´Ù¾çÇÑ serotypeÀÇ AAV ÃßÃâ¿¡ Àû¿ë °¡´É
  • ³ôÀº recovery È¿À²
  • Ultracentrifugation°ú °°Àº º¹ÀâÇÑ °úÁ¤ ºÒÇÊ¿ä
  • AAVpro¢ç Purification Pack Maxi (All Serotypes) (Code 6678)°ú ÇÔ²² »ç¿ëÇϸé, ÃßÃâºÎÅÍ Á¤Á¦±îÁö 4½Ã°£ ³» ¿Ï·á
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­, ºñºÐ¿­ ¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡¼­´Â Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ, ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÀÌ´Ù. ÀÌ·¯ÇÑ AAV¸¦ ½ÇÇè µ¿¹°¿¡ µµÀÔÇÒ ¶§¿¡´Â, packaging cell°ú ¹è¾ç ¹èÁö·ÎºÎÅÍ À¯·¡ÇÑ ºÒ¼ø¹°µéÀ» Á¦°ÅÇØ¾ß ÇÑ´Ù.

AAVpro¢ç Freeze-Thaw Extraction Buffer (All Serotypes)´Â harvest Á÷ÈÄ È¤Àº frozen »óÅÂÀÇ AAV packaging cell·ÎºÎÅÍ Freeze-thaw ¹æ½ÄÀ¸·Î AAV¸¦ ÃßÃâÇس»±â À§ÇÑ ½Ã¾àÀÌ´Ù. Packaging cellÀ» º» ½Ã¾àÀ¸·Î suspensionÇÑ ÈÄ ´Ü ÇѹøÀÇ freeze-thaw °úÁ¤À¸·Î ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ È¿°úÀûÀ¸·Î ÃßÃâÇÒ ¼ö ÀÖ´Ù.

AAVpro¢ç Purification Pack Maxi (All Serotypes) (Code 6678)¿Í ÇÔ²² »ç¿ëÇϸé ultracentrifugation°ú °°Àº º¹ÀâÇÑ °úÁ¤ ¾øÀÌ, 4½Ã°£ ³» °£ÆíÇÏ°Ô Á¤Á¦ ¹× ³óÃàµÈ AAV¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
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1) AAVÀÇ Á¤Á¦ ¹× ¼øµµ ÃøÁ¤
ZsGreen1 Çü±¤´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â °¢ serotypeÀÇ AAV (AAV1, AAV2, AAV5, AAV6)¸¦ packaging ÇßÀ¸¸ç, AAVpro¢ç Freeze-Thaw Extraction Buffer (All Serotypes) (Code 6679)·Î ¹ÙÀÌ·¯½º¸¦ ÃßÃâÇÑ µÚ AAVpro¢ç Purification Pack Maxi (All Serotypes) (Code 6678)·Î Á¤Á¦ÇÏ¿´´Ù. ÀÌ·¸°Ô Á¤Á¦µÈ AAV´Â AAVpro¢ç Titration Kit (for Real Time PCR) (Code 6233)·Î ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿©, 1X109 vg/lane À¸·Î SDS-PAGE¸¦ ¼öÇàÇÏ¿´´Ù.
±× °á°ú, °¢ serotype º° ¸ðµç AAV¿¡¼­ AAV capsid proteinÀÎ VP1, VP2, VP3 band¸¦ È®ÀÎÇÏ¿´´Ù.

2) Á¤Á¦µÈ AAVÀÇ °¨¿° È¿À² ºñ±³
1)¿¡¼­ ¾òÀº Á¤Á¦µÈ serotype º° AAV¸¦ 293T cell°ú HeLa cell¿¡ °¢°¢ transduction ÇÑ ÈÄ °¨¿° È¿À²À» È®ÀÎÇÏ¿´´Ù. Á¤Á¦ÇÑ AAV¸¦ °¢ ¼¼Æ÷¿¡ 10,000 vg/cell (AAV1, AAV2) ȤÀº 100,000 vg/cell (AAV5, AAV6)·Î °¨¿°½ÃŲ 3ÀÏ ÈÄ flow cytometry·Î ºÐ¼®ÇÑ °á°ú, º» Á¦Ç°À» ÀÌ¿ëÇØ Á¤Á¦ÇÑ ÈÄ¿¡µµ °¨¿° ´É·ÂÀ» À¯ÁöÇÏ°í ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.
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AAVpro Freeze-Thaw Extraction Buffer (All serotypes)

20 §¢ X 2

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Keyword : AAV,adeno-associatedvirus,Freeze-thaw,freezethaw,in-vivo,invivo,invivo,Àκñº¸,extraction,ÃßÃâ,purification,Á¤Á¦,ultracentrifuge,invivo,immunogenicity,¸é¿ª¿ø¼º,µ¿¹°½ÇÇè,½ÇÇ赿¹°

Adeno-Associated Virus (AAV)
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Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
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Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
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Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
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¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
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Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
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Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
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SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
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AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
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Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
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´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
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AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
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AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
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ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
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°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
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±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution