´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > Tet One Inducible Expression (One vector)
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ½Ã½ºÅÛ

Tet One Inducible Expression (One vector)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631844
Lenti-X¢â Tet-One¢â Inducible Expression System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
Each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32788
Clontech
631847
Lenti-X¢â Tet-One¢â Inducible Expression System (Puro)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
Each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32788

Ư¡
  • °­·ÂÇÏ°Ô ¹ßÇöÀ» Á¶Àý ÇÒ ¼ö ÀÖ´Â Tet-On¢ç 3G systemÀ» ±â¹ÝÀ¸·Î ÇÑ all-in-one vectorÇüÅÂ
  • ³·Àº ¹é±×¶ó¿îµå¿Í ³ôÀº À¯µµ¹ßÇö È¿À²
  • 10 ng/mlÀÇ doxycycline¿¡µµ ¹ÝÀÀÇÏ´Â ¹Î°¨µµ
  • °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ÆÐŰ¡ ½Ã½ºÅÛ Æ÷ÇÔ (Lenti-X¢â Packaging single shots)
Á¦Ç°¼³¸í
Tet-One¢â SystemÀÌ °³¹ßµÇ±â Àü¿¡´Â ClontechÀÇ Tet-on, Tet-off systemÀº response vector¿Í regulatory vector(doxycycline-responsive transactivator protein)ÀÎ 2°³ÀÇ vector¸¦ À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇß´Ù.

Tet-One¢â SystemÀº ÇϳªÀÇ º¤ÅÍ¿¡ ±¸¼º¿ä¼Ò¸¦ ¸ðµÎ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Tet-On 3G transactivator´Â human phosphoglycerate kinase 1 promoter¸¦ ÀÌ¿ëÇÏ¿© Á¤¹æÇâÀ¸·Î ¹ßÇöµÇ¸ç, ¹Ý´ë ¹æÇâ¿¡´Â PTRE3GS promoter¸¦ ÀÌ¿ëÇÏ¿© ¸ñÀû À¯ÀüÀÚ(Gene of interest)ÀÇ ¹ßÇöÀ» Á¶ÀýÇÑ´Ù. ±âÁ¸¿¡ ¹ßÇ¥µÈ all-in-one vector systemÀº 50~100¹è Á¤µµÀÇ À¯µµ¹ßÇö È¿À²À» º¸ÀδÙ. ÇÏÁö¸¸ ClontechÀÇ Tet-One¢â systemÀº all-in-one vector ÇüÅ·ΠÃÖ´ë 2500¹èÀÇ À¯µµ¹ßÇö È¿À²À» º¸ÀδÙ.(Heinz et al, 2011).


±×¸². Tet-One¢â System Mechanism - The Tet-On 3G transactivator protein is expressed constitutively from the human PGK promoter (PPGK) but is unable to bind to the TRE3G promoter (PTRE3G) in the absence of doxycycline (Dox). When bound by Dox, supplied in the culture medium, the transactivator undergoes a conformational change, binds to PTRE3G and activates transcription of a transgene cloned downstream.


±×¸². Seven out of eight Tet-One¢â clones show more than 1,000-fold induction of expression. HeLa cells were infected with LVX-TetOne-Puro-Luc lentivirus (M.O.I. = 1) and eight individual clones were selected and expanded according to the protocol. Each clone was analyzed for doxycycline-induced expression of luciferase. Seven of the eight clones demonstrated more than 1,000-fold induced expression, including two clones with 5,000-fold induction, and one clone with 10,000-fold induction.

±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
* Lenti-X¢â Tet-One¢â Inducible Expression System (Code 631844)

* Lenti-X¢â Tet-One¢â Inducible Expression System (Puro) (Code 631847)




Keyword : Tet À¯µµ¹ßÇö ½Ã½ºÅÛ,Tet-inducible,Tetracycline,Å×Æ®¶ó»çÀÌŬ¸°

Lentivirus
>
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
>
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
>
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
>
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
>
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
>
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
>
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
>
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
>
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
>
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
>
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
>
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
>
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
>
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
>
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
>
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
>
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
>
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
>
ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mi..
TransIT¢ç Lentivirus System