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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ - Ãʱ⠼¼Æÿ¡ ÃÖÀû

CMV promoter º¤ÅÍ ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631253
Lenti-X¢â Expression System (EF1a Version)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
set
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x33433, x33847
Clontech
632164
Lenti-X¢â Expression System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Set
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x32778, x33200
Clontech
632181
Lenti-X¢â Bicistronic Expression System (Neo)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x32778, x33200
Clontech
632182
Lenti-X¢â Bicistronic Expression System (Hyg)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x32778, x33200
Clontech
632183
Lenti-X¢â Bicistronic Expression System (Puro)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x32778, x33200
Clontech
631275
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,460,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631276
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
4,278,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631277
Lenti-X¢â Packaging Single Shots (Integrase Deficient)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,878,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631278
Lenti-X¢â Packaging Single Shots (Ecotropic)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,878,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778



  • ÃÖÀûÈ­¸¦ ÅëÇÑ °í¹ßÇö°ú °í¿ª°¡ ½ÇÇö
  • ´ÜÁö 1¥ìlÀÇ ¼ø¼öÇÑ ¹ÙÀÌ·¯½º »óÃþ¾× ¸¸À¸·Îµµ 10 cm dishÀÇ ¸ðµç ¼¼Æ÷¿¡ µµÀÔ °¡´É
  • WPRE°ú cPPT element°¡ µµÀÔÈ¿À²°ú ¹ßÇöÀ²À» Áõ°¡½ÃÅ´
  • Lenti-X¢â HTX Packaging Single shot ÀÌ Æ÷ÇÔµÊ
HIV-1ºÎÅÍ À¯·¡µÈ lentivirus vector´Â primary culture, ºñºÐ¿­¼¼Æ÷, Áٱ⼼Æ÷ ¹× ½Å°æ¼¼Æ÷¸¦ Æ÷ÇÔÇÑ °ÅÀÇ ¸ðµç Æ÷À¯·ù ¼¼Æ÷¿¡ À¯ÀüÀÚ¸¦ µµÀÔÇÒ ¼ö ÀÖ´Ù.

ÃÖ°í È¿À²ÀÇ Lentivirus vector
ClontechÀÇ ¸ðµç Lenti-X¢â vector (Figure 1)´Â lentivirus LTR°ú packaging ¼­¿­ ¿Ü¿¡, ¹ÙÀÌ·¯½º ¿ª°¡ ¶Ç´Â º¹Á¦ µîÀ» Å©°Ô Çâ»ó½ÃÅ°´Â Ưº°ÇÑ ¿ä¼ÒµéÀ» °¡Áö°í ÀÖ´Ù.
  • WPRE - Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)´Â RNAÀÇ processing°ú ÇÙ¿Ü·ÎÀÇ À̵¿ (nuclear export)À» ÃËÁø½ÃÅ°¸ç (1), viral genomic RNA¿¡ ÀÛ¿ëÇÏ¿© vector pacakaging È¿À²°ú ¹ÙÀÌ·¯½º ¿ª°¡¸¦ ³ô¿©ÁØ´Ù. ¶ÇÇÑ, mRNA Àü»ç¸¦ ÃËÁø½ÃÅ´À¸·Î½á ¸ñÀû ¼¼Æ÷¿¡¼­ ½ÇÇèÀÚÀÇ cDNA transgeneÀÇ ¹ßÇöÀ» Áõ°¡½ÃŲ´Ù.
  • cPPT/CTS - Central polypyrimidine tract/central termination sequence (cPPT/CTS)´Â ¸ñÀû ¼¼Æ÷¸¦ °¨¿°½ÃÅ°´Â µ¿¾È viral genomeÀÌ ÇÙ ³»·Î À¯ÀÔ µÇ´Â °ÍÀ» ÃËÁø½ÃÄÑ vectorÀÇ integration°ú ÇüÁúµµÀÔ È¿À²À» Áõ°¡½ÃŲ´Ù (2).
  • RRE - Rev-responsive element (RRE)´Â splicing °úÁ¤À» °ÅÄ¡Áö ¾ÊÀº viral RNA (unspliced virus RNA)ÀÇ ÇÙ¿Ü·ÎÀÇ À̵¿À» Áõ°¡½ÃÄÑ ¹ÙÀÌ·¯½º ¿ª°¡¸¦ Çâ»ó½ÃŲ´Ù.

Figure 1. Lenti-X¢â Expression Systems and Vectors. Lenti-X¢â vectors contain essential packaging sequences (lentiviral LTRs, the ¥× packaging sequence, etc.) and additional elements that facilitate packaging and/or boost transgene expression. The Lenti-X¢â Expression System (pLVX-Puro Vector) is designed for constitutive expression of a gene of interest and provides a puromycin selectable marker. The Lenti-X¢â Bicistronic Expression Systems allow your protein and a selectable marker to be coexpressed from a single mRNA transcript.
Lentiviral Packaging Systems-3°¡Áö ¿É¼Ç

°í¿ª°¡ÀÇ Lentivirus¸¦ Á¦ÀÛ
Lenti-X¢â Packaging Single shots

Lenti-X¢â Packaging Single shotsÀº ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡ Çʼö ±¸¼º À¯ÀüÀÚ°¡ ÃÖÀûÀÇ ºñÀ²·Î Æ÷ÇÔµÇ¾î ¹ÙÀÌ·¯½º »ý»êÀ» ±Ø´ëÈ­Çϸç, ¶ÇÇÑ ÀÌ ±¸¼º¿ä¼ÒµéÀÌ °¢°¢ÀÇ ºÐ¸®µÈ vector·Î µÇ¾î ÀÖ¾î ¹ÙÀÌ·¯½º ÀÚ°¡ º¹Á¦ °¡´É¼ºÀ» È¿°úÀûÀ¸·Î ¾ïÁ¦ÇÏ¿© ¾ÈÁ¤¼ºÀ» È®º¸Çß´Ù.

1. VSV-G lentiviral packaging-Ç¥ÁØÀÇ ÀϹÝÀûÀÌ°í °¡Àå ¼±È£µÇ´Â Lenti-X¢â Packaging Single shots (VSV-G) (Code 631275) Àº VSV-G pseudotyped lentivirus¸¦ Á¦ÀÛÇϴµ¥ ÀÌ¿ëµÇ¸ç °ÅÀÇ ¸ðµç Á¾·ùÀÇ ¼¼Æ÷¿¡ °¨¿°½Ãų ¼ö ÀÖ´Ù.

2. Ecotropic lentiviral packaging - Lenti-X¢â Packaging Single shots (Ecotropic) (Code 631278) Àº MLV ecotropic envelope glycoproteinÀ» °¡Áø lentivirus¸¦ »ý»êÇϴµ¥ ÀÌ°ÍÀº mouse cellÀ̳ª rat cells¿¡ trasnsduction½Ã À¯¿ëÇÏ´Ù.

3. Lenti-X¢â Packaging Single shots (Integrase Deficient) (Code 631277) ÀºIDLV generationÀ» À§ÇÑ 4¼¼´ë lentiviral packaging system Àü¿ëÀ¸·Î Lenti-X¢â 293T ¼¼Æ÷¿¡ ÇüÁúÀüȯÇؼ­ ÃÖ´ë ¿ª°¡titerÀÇ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖµµ·Ï ÃÖÀûÈ­µÈ ºñÀ²·Î 5°³ÀÇ plasmid¸¦ Á¶ÇÕÇØ ³õÀº ½Ã½ºÅÛÀÌ´Ù.

Figure 2. Highly efficient transduction. 293T cells were infected with the indicated volumes (¥ìl) of a Lenti-X¢â supernatant generated using the Lenti-X¢â Packaging System and the pLVX-Puro vector. After puromycin selection, resistant colonies were stained with crystal violet.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Á¦Ç° º° vector Á¤º¸

Vector¸í

Á¤º¸

pLVX-Puro Vector (#632164 ±¸¼ºÇ°)

Vector map

Sequence (txt.)

pLVX-EF1alpha-IRES-Puro Vector (#631253 ±¸¼ºÇ°)

Vector map

Sequence (txt.)

pLVX-IRES-Neo vector (#632181 ±¸¼ºÇ°)

Vector map

Sequence (txt.)

pLVX-IRES-Hyg Vector (#632182 ±¸¼ºÇ°)

Vector map

Sequence (txt.)

pLVX-IRES-Puro Vector (#632183 ±¸¼ºÇ°)

Vector map

Sequence (txt.)

References
1. Zufferey, R. et al. (1999) J. Virol. 73(4):2886-2892.
2. Zennou, V. et al. (2000) Cell 101(2):173-185.
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>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)




Keyword :

Lentivirus
>
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
>
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
>
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
>
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
>
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
>
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
>
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
>
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
>
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
>
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
>
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
>
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
>
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
>
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
>
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
>
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
>
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
>
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
>
ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mi..
TransIT¢ç Lentivirus System