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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > Packaging Cell £¦ ½Ã½ºÅÛ

Packaging Cell £¦ ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631530
Retro-X Universal Packaging System
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
1 Set
1,319,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33346, x33615, x33224, x33265, x33005, x33178
Clontech
631505
AmphoPack-293 Cell Line
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
1 ml
1,453,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33005, x33178
Clontech
631512
Pantropic Retroviral Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
1 Set
1,493,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33410, x33485, x33005
Clontech
631510
RetroPack PT67 Cell Line
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
1 ml
1,404,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33005

[ÁÖÀÇ] µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ ÇØµ¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
       ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.

  • °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retroivirus¸¦ È¿°úÀûÀÌ°í ½Å¼ÓÇÏ°Ô »ý»ê
  • ´Ù¾çÇÑ ¼¼Æ÷¿¡ °¨¿°
Retro-XTM Universal Packaging System
Retro-X Universal Packaging SystemÀº GP2-293 packaging cell lines°ú ¸ñÀûÀ¸·Î ÇÏ´Â cell line¿¡ µû¶ó packaged virus°¡ ģȭ¼ºÀ» º¸À̵µ·Ï ÇÏ´Â 4 °³ÀÇ envelope vector (pVSV-G, pEco, pAmpho, p101A1)·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, °í¿ª°¡ÀÇ amphotropic, ecotropic, dualtropic ¹× pantropic virus¸¦ 48 ½Ã°£ À̳»¿¡ ¾òÀ» ¼ö ÀÖ´Ù. ÀÌ packaging systemÀº Pantropic Expression System°ú °°Àº cell lines¿¡ »ç¿ëÇÑ´Ù.
AmphoPackTM 293 cell lines
AmphoPack 293Àº ´Ù¾çÇÑ Æ÷À¯·ù ¼¼Æ÷¿¡ °¨¿°½ÃŰ´Â ÀçÁ¶ÇÕ MMLV °è¿­ÀÇ ¹ÙÀÌ·¯½º ÀÔÀÚ¸¦ »ý»êÇϴµ¥ »ç¿ëµÈ´Ù.
AmphoPack 293Àº HEK 293 °è¿­ÀÇcell lineÀ¸·Î ½±°Ô transfectionµÇ¸ç 106 cfu/ml¸¦ ÃʰúÇÏ´Â ¹ÙÀÌ·¯½º ¿ª°¡¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù. ¹ÙÀÌ·¯½ºÀÇ envelope ´Ü¹éÁúÀº amphotropic receptor (Ram-1)¸¦ ÀÎÁöÇÑ´Ù.
Pantropic Retroviral Expression System
Pantropic Retroviral Expression SystemÀº VSV-G virus envelope glyco proteinÀ» ¹ßÇöÇÏ´Â ¹ÙÀÌ·¯½º¸¦ »ý»êÇϱâ À§ÇÏ¿© GP2-293 cell lineÀ» »ç¿ëÇÑ´Ù. ÀÌ glycoproteinÀº ¼¼Æ÷ Ç¥¸éÀÇ receptor¿¡ ÀÇÁ¸ÀûÀÌÁö´Â ¾ÊÁö¸¸ lipid °áÇÕ ÇÏ¿© plasma membrane°úÀÇ À¶ÇÕÇÏ¿© ¹ÙÀÌ·¯½º°¡ ħÅõÇÏ´Â °ÍÀ» ¸Å°³ÇÑ´Ù (2).
RetroPackTM PT67 cell lines
RetroPack PT67´Â 10A1 viral envelop¸¦ ¹ßÇö½ÃŰ´Â NIH3T3 ±â¹ÝÀÇ packaging cell lineÀÌ´Ù. PT67 ¼¼Æ÷¿¡¼­ packagingµÈ ¹ÙÀÌ·¯½º´Â ´Ù¾çÇÑ Æ÷À¯·ù ¼¼Æ÷¸¦ °¨¿°½Ã۴µ¥ »ç¿ëµÈ´Ù. ¿Ö³ÄÇÏ¸é ¹ÙÀÌ·¯½º°¡ µÎ °³ÀÇ ´Ù¸¥ Ç¥¸é ºÐÀÚÀÎ amphotropic retroivirus receptor (Ram-1)¿Í GALVr receptor¸¦ ÅëÇØ ¼¼Æ÷³»·Î µé¾î°¥ ¼ö Àֱ⠶§¹®ÀÌ´Ù. RetroPack Cell LineÀº Retro-X system¿¡ Æ÷ÇԵǾî ÀÖ´Ù.
retroivirus packaging cell line°ú systemÀº ClontechÀÇ ¸ðµç retroivirus vector¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Table I: Retroviral Packaging Cell Lines
Integrated Markers
Product Cell Type Tropism Envelope Receptors gag-pol env Host Cell
AmphoPack 293 Cell Line HEK 293 Amphotropic 4070A Ram-1 (rPit-2) Bleo Puro Many mammalian cell types
RetroPack PT67 Cell Line NIH 3T3 Dualtropic 10A1 GALVr, Ram-1 TK DHFR Many mammalian cell types
GP2-293 Cell Line* HEK 293 Pantropic ** ** ** n/a All cell types
* Retro-X Universal Packaging System and the Pantropic Retroviral Expression System.
** The GP2-293 Cell Line requires cotransfection with one of several types of envelope protein vectors and can produce virus with various tropisms.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
1. Burns, J. C. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8033-8037.
2. Emi, N. et al. (1991) J. Virol. 65:1202-1207.

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