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Gene transfer (virus)
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Retrovirus
> Packaging Cell £¦ ½Ã½ºÅÛ
Packaging Cell £¦ ½Ã½ºÅÛ
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Clontech
631530
Retro-X Universal Packaging System
1 Set
(Package)
1,665,000¿ø
,
,
,
,
,
Clontech
631512
Pantropic Retroviral Expression System
1 Set
(Package)
1,770,000¿ø
,
,
Clontech
631510
RetroPack PT67 Cell Line
1 ml
1,460,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
[ÁÖÀÇ]
µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE:
We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
°í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retroivirus¸¦ È¿°úÀûÀÌ°í ½Å¼ÓÇÏ°Ô »ý»ê
´Ù¾çÇÑ ¼¼Æ÷¿¡ °¨¿°
Retro-X
TM
Universal Packaging System
Retro-X Universal Packaging SystemÀº GP2-293 packaging cell lines°ú ¸ñÀûÀ¸·Î ÇÏ´Â cell line¿¡ µû¶ó packaged virus°¡ ģȼºÀ» º¸À̵µ·Ï ÇÏ´Â 4 °³ÀÇ envelope vector (pVSV-G, pEco, pAmpho, p101A1)·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, °í¿ª°¡ÀÇ amphotropic, ecotropic, dualtropic ¹× pantropic virus¸¦ 48 ½Ã°£ À̳»¿¡ ¾òÀ» ¼ö ÀÖ´Ù. ÀÌ packaging systemÀº Pantropic Expression System°ú °°Àº cell lines¿¡ »ç¿ëÇÑ´Ù.
Pantropic Retroviral Expression System
Pantropic Retroviral Expression SystemÀº VSV-G virus envelope glyco proteinÀ» ¹ßÇöÇÏ´Â ¹ÙÀÌ·¯½º¸¦ »ý»êÇϱâ À§ÇÏ¿© GP2-293 cell lineÀ» »ç¿ëÇÑ´Ù. ÀÌ glycoproteinÀº ¼¼Æ÷ Ç¥¸éÀÇ receptor¿¡ ÀÇÁ¸ÀûÀÌÁö´Â ¾ÊÁö¸¸ lipid °áÇÕ ÇÏ¿© plasma membrane°úÀÇ À¶ÇÕÇÏ¿© ¹ÙÀÌ·¯½º°¡ ħÅõÇÏ´Â °ÍÀ» ¸Å°³ÇÑ´Ù (2).
RetroPack
TM
PT67 cell lines
RetroPack PT67Àº 10A1 viral envelop¸¦ ¹ßÇö½ÃÅ°´Â NIH3T3 ±â¹ÝÀÇ packaging cell lineÀÌ´Ù. PT67 ¼¼Æ÷¿¡¼ packagingµÈ ¹ÙÀÌ·¯½º´Â ´Ù¾çÇÑ Æ÷À¯·ù ¼¼Æ÷¸¦ °¨¿°½ÃÅ°´Âµ¥ »ç¿ëµÈ´Ù. ¿Ö³ÄÇÏ¸é ¹ÙÀÌ·¯½º°¡ µÎ °³ÀÇ ´Ù¸¥ Ç¥¸é ºÐÀÚÀÎ amphotropic retroivirus receptor (Ram-1)¿Í GALVr receptor¸¦ ÅëÇØ ¼¼Æ÷³»·Î µé¾î°¥ ¼ö Àֱ⠶§¹®ÀÌ´Ù. RetroPack PT67 Cell LineÀº Retro-X system¿¡ Æ÷ÇԵǾî ÀÖ´Ù.
Retroivirus packaging cell line°ú systemÀº ClontechÀÇ ¸ðµç retroivirus vector¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Table I: Retroviral Packaging Cell Lines
Integrated Markers
Product
Cell Type
Tropism
Envelope
Receptors
gag-pol
env
Host Cell
RetroPack PT67 Cell Line
NIH 3T3
Dualtropic
10A1
GALVr, Ram-1
TK
DHFR
Many mammalian cell types
GP2-293 Cell Line*
HEK 293
Pantropic
**
**
**
n/a
All cell types
* Retro-X Universal Packaging System and the Pantropic Retroviral Expression System.
** The GP2-293 Cell Line requires cotransfection with one of several types of envelope protein vectors and can produce virus with various tropisms.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
1. Burns, J. C. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8033-8037.
2. Emi, N. et al. (1991) J. Virol. 65:1202-1207.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit