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Soluble tagÀÇ °áÁ¤ÆÇ

pCold¢â ProS2 DNA

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3371
pCold¢â ProS2 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
943,200¿ø 
1,179,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3371_e.v1909Da.pdf



  • ±×·¥ À½¼ºÀÇ Á¡¾× ¼¼±Õ Myxococcus xanthus¿¡¼­ À¯·¡ÇÑ Protein S¸¦ °¡¿ëÈ­ ű׷ΠÀÌ¿ë
  • ProS2ÅÂ±× À¶ÇÕ ¹ßÇö¿¡ ÀÇÇÑ °¡¿ë¼ºÀÌ Çâ»ó
  • HisÅÂ±× Á¤Á¦, °¡¿ëÈ­ ű×ÀÇ Àý´ÜÀÌ °¡´É

*º» Á¦Ç°±ºÀ» »ó¾÷Àû ¿ëµµ·Î ±¸¸ÅÇÏ´Â °æ¿ì ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
Á¦Ç°¼³¸í
pCold¢â ProS2 DNA´Â ±×¶÷À½¼º Á¡¾×¼¼±Õ Myxococcus xanthus À¯·¡ÀÇ Protein S¸¦ soluble tag·Î »ç¿ëÇÏ´Â »õ·Î¿î pCold¢â vector ½Ã¸®ÁîÀÌ´Ù. 173°³ÀÇ ¾Æ¹Ì³ë»êÀ¸·Î ±¸¼ºµÈ Protein S´Â Myxococcus xanthus °¡ Çü¼ºÇÏ´Â Æ÷ÀÚÀÇ ¿Ü°¢¿¡ Á¸ÀçÇÏ¸ç ¸Å¿ì ¾ÈÁ¤ÀûÀÎ °¡¿ë¼º ´Ü¹éÁúÀÌ´Ù. Protein SÀÇ NTD (N - terminal domain)À» º´·Ä·Î 2°³ ¿¬°áÇÑ ProS2 tag (¾à 23 kDa)¸¦ ¸ñÀû ´Ü¹éÁúÀÇ N ¸»´Ü¿¡ À¶ÇÕ ¹ßÇö½ÃÅ´À¸·Î½á, À¶ÇÕ ´Ü¹éÁúÀÇ ¾ÈÁ¤¼º, °¡¿ë¼ºÀ» Çâ»ó½Ãų ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸ñÀû´Ü¹éÁú°úÀÇ »óÈ£ ÀÛ¿ëÀÌ ³·±â ¶§¹®¿¡ ¸ñÀû´Ü¹éÁú ºÎºÐ°ú soluble tag (ProS2)¸¦ È¿À²ÀûÀ¸·Î ºÐ¸®ÇÒ ¼ö ÀÖ´Ù.
º» Vector´Â
cspA promoterÀÇ down-stream¿¡ 5¡¯ untranslated region (5'UTR), translation enhancing element (TEE), His tag, ProS2 tag, multi-cloning site (MCS) µîÀÌ ¹èÄ¡µÇ¾î ÀÖ´Ù (±×¸² ÂüÁ¶). TEE´Â ¹ø¿ªÀ» ÃËÁøÇÏ´Â ¿ªÇÒÀ» ÇÏ°í, His tag´Â ¹ßÇö ´Ü¹éÁúÀÇ Á¤Á¦¿¡ È°¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ¶ÇÇÑ Promoter downstream¿¡´Â ¹ßÇöÀ» Á¦¾îÇϱâ À§ÇÑ lac operator°¡ »ðÀԵǾî ÀÖ´Ù.

¶ÇÇÑ ProS2 tag¿Í MCS »çÀÌ¿¡´Â HRV 3C Protease, Thrombin, Factor Xa Àνļ­¿­ÀÌ ÀÖ¾î ¹ßÇö ÈÄ À¶ÇÕ ´Ü¹éÁú¿¡¼­ tag¸¦ Á¦°ÅÇÒ ¼ö ÀÖ°í, E. coli promoter¸¦ ÀÌ¿ëÇÏ°í Àֱ⠶§¹®¿¡ ´Ù¸¥ pCold¢â DNA ½Ã¸®Áî¿Í ¸¶Âù°¡Áö·Î ´ëºÎºÐÀÇ E. coli strainÀ» host·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.
ÀÌ¿Í °°ÀÌ pCold¢â ProS2 DNA ¸¦ ÀÌ¿ëÇϸé Cold Shock vector ½Ã¸®ÁîÀÇ Æ¯Â¡ÀÎ ´Ü¹éÁú °íÈ¿À² ¹ßÇö ´É·Â°ú ProS2ÀÇ soluble tag ±â´É ¹× È¿À²ÀûÀÎ ºÐ¸®¸¦ ÅëÇØ ¼Õ½±°Ô ´Ü¹éÁú ¹ßÇö Á¤Á¦ ½Ã½ºÅÛÀ» ±¸ÃàÇÒ ¼ö ÀÖ´Ù.


±×¸². pCold¢â ProS2 vector map
º¸Á¸
-20 ¡É
pCold¢â ProS2 vector sequence
pCold ProS2.fasta
»ç¿ë »ó ÁÖÀÇ»çÇ×
º» Á¦Ç°Àº ¿¬±¸ ¸ñÀûÀ¸·Î¸¸ »ç¿ë °¡´ÉÇÑ Á¦Ç°ÀÔ´Ï´Ù (Research use only).
º» Á¦Ç° ¶Ç´Â º» Á¦Ç°À¸·Î ¸¸µé¾îÁø »ê¹°À» »ó¾÷Àû ¸ñÀûÀ¸·Î ÀÌ¿ëÇÏ´Â °æ¿ì, ¶óÀ̼±½º°¡ Æ÷ÇÔµÈ Á¦Ç°À» ±¸ÀÔÇÏ½Ç ¼ö ÀÖÀ¸´Ï ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
°ü·ÃÁ¦Ç°
- ÀçÁ¶ÇÕ ´Ü¹éÁú ¹ßÇö¿ë E. coli Comp. cell : TaKaRa Competent Cells BL21
- Â÷¼¼´ë Membrane ±â¼úÀÇ His-tagged protein Á¤Á¦ column : Capturem¢â His-Tagged Purification
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî

Keyword :

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
>
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
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ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
>
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
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Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
>
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
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°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
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¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)