´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > Overview > [°³¿ä] Â÷¼¼´ë ´Ü¹éÁú Á¤Á¦, Capturem¢â

[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú Á¤Á¦, Capturem¢â

-

[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú Á¤Á¦, Capturem¢â

´Ù¾çÇÑ Á¾·ùÀÇ ´Ü¹éÁúÀº ÇÕ¼º ¹× Á¤Á¦ÇÏ´Â ¹æ¹ý¿¡ µû¶ó ½ÇÇè °á°úÀÇ Â÷À̸¦ º¸ÀÏ ¼ö ÀÖ´Ù. Capturem¢â ±â¼úÀº Spin column ¹æ½ÄÀ¸·Î, ´Ù¾çÇÑ ´Ü¹éÁú°ú Ç×ü¸¦ ´Ü½Ã°£ ³» °í¼øµµ, °íÈ¿À²·Î Á¤Á¦, ÃßÃ⠵ Àû¿ëÇÒ ¼ö ÀÖ´Ù. Spin column¿¡´Â novel nylon-based membraneÀÌ °áÇյǾîÀÖ¾î ´õ¿í ³ôÀº protein binding capacity¸¦ °¡Áú ¼ö ÀÖ´Ù. µû¶ó¼­, ³ÐÀº ¸éÀûÀ¸·Î Capacity°¡ Å©±â¿¡ Ç¥¸éÀû°ú Å« ¿ë·® (Capacity)ÀÇ Æ¯¼ºÀ» °¡Áö¸ç, ¸¹Àº ½Ã°£°ú ³ë·ÂÀÌ ÇÊ¿äÇß´ø ±âÁ¸ ¹æ¹ý¿¡ ºñÇØ ºü¸¥ ½Ã°£ ³»¿¡ ¸· Åë°ú°¡ °¡´ÉÇØ º¯¼ºÀÌ ¾ø´Â ´Ü¹éÁúÀ» Á¤Á¦ ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ³·Àº ¾Ð·ÂÀ¸·Î ¿ë¾×ÀÌ Åë°úÇÒ ¼ö ÀÖ¾î ´Ü¹éÁúÀÇ Ç°Áú ÀúÇϸ¦ ¹æÁöÇϸç, bed volumeÀ¸·Î °í³óµµ, °í¼øµµ·Î Á¤Á¦µÈ ´Ü¹éÁúÀ» ¾òÀ» ¼ö ÀÖ´Ù.

Capturem¢â Workflow
Capturem¢â ±â¼úÀ» ÀÌ¿ëÇÑ His-tagged protein Á¤Á¦
- Capturem¢âÀ» ÀÌ¿ëÇÑ His-tagged protein Á¤Á¦ Á¦Ç°
 
Code
ÇüÅÂ

Miniprep Column

Maxiprep column

96 Nickel plate

24 Nickel plate

Large volume unit
Input
sample
~ 800 §¡
~ 25 §¢
~ 1,000 §¡
~ 4 §¢
150 ~ 500 §¢
¿ë·®
20 ȸ
6 ȸ
96 well
24 well
4 ȸ
¼Ò¿ä½Ã°£
5 ºÐ
15 ºÐ
15 ºÐ
15 ºÐ
15 ~ 30 ºÐ
Á¤Á¦³óµµ
0.3 ~ 1 §·/§¢
1.6 ~ 4.5 §·/§¢
0.3 ~ 1 §·/§¢
~ 0.8 §·/1§¢
up to 2.0 §·/§¢
Á¤Á¦¼öÀ²***
80§¶/column
1.5 §·/tube
80§¶/well
800§¶/well
10~25 §·/unit
Centrifuge
Á¶°Ç
11,000 x g, 1ºÐ
2,000 x g, 3ºÐ*
2,000 x g, 3ºÐ*
600 x g, 2ºÐ*
X
Vacuum
¿©ºÎ
X
X
O
O
O**
Buffer
Æ÷ÇÔ ¿©ºÎ
O
O
X
X
X

* Maxiprep Kit¿¡´Â 50§¢ tube¿ë, 24well plate, 96well plate¿¡´Â °¢°¢ 24well plate, 96well plate¿ë centrifuge°¡ ÇÊ¿äÇÕ´Ï´Ù.
** Large volumeÀÇ °æ¿ì, Vacuum source (e.g. vacuum pump equipped with a trap and a vacuum controller, or a peristaltic pump)°¡ ÇÊ¿äÇÏ´Ù.
*** Á¤Á¦¼öÀ²Àº »ç¿ëÇÏ°íÀÚ ÇϽô Sample type, species, antibody isotype¿¡ µû¶ó ´Þ¶óÁú ¼ö ÀÖ½À´Ï´Ù.