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> ProteoGuard EDTA-Free Protease Inhibitor Cocktail
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
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Clontech
635672
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
100 ¥ìl
150,000¿ø
Clontech
635673
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
100 ¥ìl¡¿10
368,000¿ø
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
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[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
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TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
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HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
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Protease Inhibitor Cocktail
Cell lysateÀÇ proteolysis ¾ïÁ¦
xTractor Buffer³ª ´Ù¸¥ lysis buffers¿Í »ç¿ë½Ã¿¡µµ ¶Ù¾î³ È¿°ú
»ç¿ëÇϱ⠰£ÆíÇÑ ÇüÅ - single-use tubes
EDTA-free formulation - IMAC column¿¡ ¹Ù·Î Àû¿ë °¡´É
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
Àº lysis buffer¿¡ °ð¹Ù·Î ÷°¡ÇÒ ¼ö ÀÖ´Â
100 x stockÀÇ protease inhibitor
·Î endogenous proteases·ÎºÎÅÍ protein ºÐÇظ¦ ¹æÁöÇÑ´Ù. Lysis buffer 1ml ´ç 100X protease inhibitor cocktail 10 ul¸¦ ÷°¡ÇÏ¿© »ç¿ëÇÑ´Ù. EDTA-free Á¦Ç°À¸·Î Ãß°¡ÀûÀÎ gel filteration °úÁ¤¾øÀÌ IMAC resin¿¡ °ð¹Ù·Î lysate¸¦ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
Protease Inhibitor Cocktail
ProteoGuard EDTA-Free Protease Inhibitor Cocktail´Â cell lysate·ÎºÎÅÍ protein extractionÇÏ´Â °úÁ¤¿¡ endogenous proteases·ÎºÎÅÍ proteinÀÌ Æı«µÇ´Â °ÍÀ» ¹æÁöÇϵµ·Ï ÃÖÀûÈµÈ protease inhibitors·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
ÃÖÀûÈµÈ 5 Á¾ÀÇ inhibitors°¡ Æ÷ÇÔµÊ
DMSO¿¡ 100 x stockÀ¸·Î Á¦°øµÊ
Table I. Formulation of ProteoGuard EDTA-Free Protease Inhibitor Cocktail.
Applications
Protein purification
This protease inhibitor mix is compatible with IMAC resins such as TALON Co resin, His60 Ni Superflow resin, GST purification resin & other commercially available resins for recombinant protein purification
Protects proteins from degradation by suppressing proteolysis
Preserves protein biological activity by preventing degradation
Suppression of proteolysis in detergent-based cell lysis reagents
xTractor Buffer lysis reagents
Other commercially or in-house formulated cell or tissue lysis buffers
Inhibition of protease activity from lysates produced by other lysis methods
Sonication, French press, alumina tissue extraction, blender homogenization, or repetitive freeze/thaw cycling.
Keyword :
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpain ƯÀÌÀû inhibitor
Calpastatin
Àú¿Â¿¡¼ ´Ü¹éÁú ¿ë¾× ÁßÀÇ ÇÙ»ê Á¦°Å¿Í Á¡¼ºÀ»..
Cryonase¢â Cold-active Nuclease
Glutathione Excellose (GST fusion protein)
IDA Excellose (polyhistidine tagged protein)
MBP Excellose (MBP fusion protein)
MiniExcellose
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
Biotinylated proteinsÀÇ Á¤Á¦¿Í ³óÃàÀ» À§ÇÑ M..
Streptavidin-based Enrichment & Purification