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ProteoGuard EDTA-Free Protease Inhibitor Cocktail

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(ºÎ°¡¼¼º°µµ)
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Clontech
635672
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
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100 ¥ìl
150,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32967
Clontech
635673
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
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100 ¥ìl¡¿10
368,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

Protease Inhibitor Cocktail
  • Cell lysateÀÇ proteolysis ¾ïÁ¦
  • xTractor Buffer³ª ´Ù¸¥ lysis buffers¿Í »ç¿ë½Ã¿¡µµ ¶Ù¾î³­ È¿°ú
  • »ç¿ëÇϱ⠰£ÆíÇÑ ÇüÅ - single-use tubes
  • EDTA-free formulation - IMAC column¿¡ ¹Ù·Î Àû¿ë °¡´É

ProteoGuard EDTA-Free Protease Inhibitor CocktailÀº lysis buffer¿¡ °ð¹Ù·Î ÷°¡ÇÒ ¼ö ÀÖ´Â 100 x stockÀÇ protease inhibitor·Î endogenous proteases·ÎºÎÅÍ protein ºÐÇظ¦ ¹æÁöÇÑ´Ù. Lysis buffer 1ml ´ç 100X protease inhibitor cocktail 10 ul¸¦ ÷°¡ÇÏ¿© »ç¿ëÇÑ´Ù. EDTA-free Á¦Ç°À¸·Î Ãß°¡ÀûÀÎ gel filteration °úÁ¤¾øÀÌ IMAC resin¿¡ °ð¹Ù·Î lysate¸¦ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
Protease Inhibitor Cocktail
ProteoGuard EDTA-Free Protease Inhibitor Cocktail´Â cell lysate·ÎºÎÅÍ protein extractionÇÏ´Â °úÁ¤¿¡ endogenous proteases·ÎºÎÅÍ proteinÀÌ Æı«µÇ´Â °ÍÀ» ¹æÁöÇϵµ·Ï ÃÖÀûÈ­µÈ protease inhibitors·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
  • ÃÖÀûÈ­µÈ 5 Á¾ÀÇ inhibitors°¡ Æ÷ÇÔµÊ
  • DMSO¿¡ 100 x stockÀ¸·Î Á¦°øµÊ


Table I. Formulation of ProteoGuard EDTA-Free Protease Inhibitor Cocktail.
Applications
  • Protein purification
    • This protease inhibitor mix is compatible with IMAC resins such as TALON Co resin, His60 Ni Superflow resin, GST purification resin & other commercially available resins for recombinant protein purification
    • Protects proteins from degradation by suppressing proteolysis
    • Preserves protein biological activity by preventing degradation
  • Suppression of proteolysis in detergent-based cell lysis reagents
    • xTractor Buffer lysis reagents
    • Other commercially or in-house formulated cell or tissue lysis buffers
  • Inhibition of protease activity from lysates produced by other lysis methods
    • Sonication, French press, alumina tissue extraction, blender homogenization, or repetitive freeze/thaw cycling.

Keyword :

´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
>
Calpain ƯÀÌÀû inhibitor
Calpastatin
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Àú¿Â¿¡¼­ ´Ü¹éÁú ¿ë¾× ÁßÀÇ ÇÙ»ê Á¦°Å¿Í Á¡¼ºÀ»..
Cryonase¢â Cold-active Nuclease
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Biotinylated proteinsÀÇ Á¤Á¦¿Í ³óÃàÀ» À§ÇÑ M..
Streptavidin-based Enrichment & Purification