´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ

pCold¢â Vector ½Ã¸®Áî

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3360
pCold¢â Vector Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
(°¢ 5 ¥ìg)
1,467,200¿ø 
1,834,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3360-3364_e.v2003Da.pdf
Takara
3361
pCold¢â I DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
558,400¿ø 
698,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3360-3364_e.v2003Da.pdf
Takara
3362
pCold¢â II DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
558,400¿ø 
698,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3360-3364_e.v2003Da.pdf
Takara
3363
pCold¢â III DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
558,400¿ø 
698,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3360-3364_e.v2003Da.pdf
Takara
3364
pCold¢â IV DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
558,400¿ø 
698,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3360-3364_e.v2003Da.pdf



  • ¹è¾ç¾×À» 15¡É¿¡ Àú¿Â ó¸® (Cold shock)ÇÏ¿© È¿À²ÀûÀÎ ¹ßÇöÀ» À¯µµ
  • ±âÁ¸ÀÇ E. coli ¹ßÇö½Ã½ºÅÛº¸´Ù, »ý»ê È¿À² ¹× °¡¿ë¼º ¹ßÇöÀÌ Çâ»ó
  • Background ¹ßÇöÀ» ³·Ãß°í ¸ñÀû´Ü¹éÁú¸¸ °íÈ¿À²·Î ¹ßÇö °¡´É
  • ´Ù¾çÇÑ E. coli strain¿¡ Àû¿ë °¡´É

º» Á¦Ç°±ºÀ» »ó¾÷Àû ¿ëµµ·Î ±¸¸ÅÇÏ´Â °æ¿ì ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº E. coli Cold Shock À¯ÀüÀÚ cspAÀÇ ÇÁ·Î¸ðÅÍ ¼­¿­°ú 5' ºñ¹ø¿ª ¼­¿­ (UTR)À» ÀÌ¿ëÇÑ »õ·Î¿î ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛÀ¸·Î, cspA ÇÁ·Î¸ðÅÍÀÇ ÇÏ·ù¿¡´Â ¹ßÇöÀ» Á¦¾îÇϱâ À§ÇÑ lac operator°¡ »ðÀԵǾî ÀÖ´Ù.
º» Vector¸¦ ÀÌ¿ëÇÏ¿© Àú¿Â¿¡¼­ ¸ñÀû´Ü¹éÁúÀÇ ¹ßÇöÀ» À¯µµÇϸé, ¼÷ÁÖ À¯·¡ ´Ü¹éÁúÀÇ ÇÕ¼ºÀº ¾ïÁ¦µÇ°í ¸ñÀû ´Ü¹éÁú¸¸À» °íÈ¿À²·Î ¾òÀ» ¼ö ÀÖ¾î ±âÁ¸ÀÇ E. coli ¹ßÇö½Ã½ºÅÛ°ú ºñ±³ÇÏ¿© ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö·®À̳ª °¡¿ë¼ºÀÇ Çâ»óÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù.
pCold vector¿¡´Â TEE* ¼­¿­, His¡¤ Tag ¼­¿­, Factor Xa Àý´Ü ¼­¿­ÀÇ À¯¹«¿¡ µû¶ó 4 Á¾·ùÀÇ pCold vector ½Ã¸®Áî°¡ ÀÖÀ¸¸ç, ¸ñÀû¿¡ µû¶ó ÃÖÀûÀÇ vector¸¦ ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.
*Translation enhancing elementÀÇ ¾àÀÚ·Î, ¹ø¿ªÀ» ÃËÁøÇÏ´Â ±â´ÉÀ» °¡Áø´Ù.

 

TEE ¼­¿­

His¡¤ Tag ¼­¿­

Factor XaÀý´Ü ¼­¿­

GenBank Accession No.

pCold I DNA

¡Û

¡Û

¡Û

AB186388

pCold II DNA

¡Û

¡Û

¡¿

AB186389

pCold III DNA

¡Û

¡¿

¡¿

AB186390

pCold IV DNA

¡¿

¡¿

¡¿

AB186391



±×¸². pCold vector map
º¸Á¸
-20 ¡É
»ç¿ë »ó ÁÖÀÇ»çÇ×
º» Á¦Ç°Àº ¿¬±¸ ¸ñÀûÀ¸·Î¸¸ »ç¿ë °¡´ÉÇÑ Á¦Ç°ÀÔ´Ï´Ù (Research use only).
º» Á¦Ç° ¶Ç´Â º» Á¦Ç°À¸·Î ¸¸µé¾îÁø »ê¹°À» »ó¾÷Àû ¸ñÀûÀ¸·Î ÀÌ¿ëÇÏ´Â °æ¿ì, ¶óÀ̼±½º°¡ Æ÷ÇÔµÈ Á¦Ç°À» ±¸ÀÔÇÏ½Ç ¼ö ÀÖÀ¸´Ï ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
°ü·ÃÁ¦Ç°
- ÀçÁ¶ÇÕ ´Ü¹éÁú ¹ßÇö¿ë E. coli Comp. cell : TaKaRa Competent Cells BL21
- Â÷¼¼´ë Membrane ±â¼úÀÇ His-tagged protein Á¤Á¦ column : Capturem¢â His-Tagged Purification
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî

Keyword :

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
>
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
>
ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
>
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
>
Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
>
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
>
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
>
¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)