TaKaRa
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TaKaRa Competent Cell BL21

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9126
TaKaRa Competent Cell BL21
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
100 ¥ìl¡¿10
292,800¿ø 
366,000¿ø
°¡°ÝÇÒÀÎ
02.26 ~ 04.19
9126_e.v2007Da.pdf

*º» Á¦Ç°Àº ¿¬±¸ ¸ñÀûÀ¸·Î¸¸ »ç¿ë °¡´ÉÇÑ Á¦Ç°ÀÔ´Ï´Ù (Research use only).
Á¦Ç°¼³¸í
E. coli BL21ÁÖ´Â lon protease, ompT ¿Ü¸· protease°¡ °á¼ÕµÈ BÁÖ À¯·¡ÀÇ ±ÕÁַνá, ¹ßÇö ´Ü¹éÁúÀÇ ¾ÈÁ¤¼ºÀ» Çâ»ó½Ãų ¼ö ÀÖ¾î ÀçÁ¶ÇÕ ´Ü¹éÁú ¹ßÇö¿¡ ³Î¸® ÀÌ¿ëµÇ°í ÀÖ´Ù.
º» Á¦Ç°Àº ƯÈ÷ Cold shock expression vectorÀÎ pCold¢â DNA ½Ã¸®Áî (Code 3360-3364), pCold¢â TF DNA (Code 3365), pCold¢â ProS2 DNA (Code 3371), pCold¢â GST DNA (Code 3372)¿Í °°ÀÌ »ç¿ëÇÏ¸é ¸ñÀû ´Ü¹éÁúÀ» ³ôÀº È¿À²·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù.

ÁÖ) º» Á¦Ç°ÀÇ ¼÷ÁÖÀÎ BL21ÁÖ´Â T7 RNA Polymerase¸¦ ¹ßÇöÇÏÁö ¾Ê¾Æ, pET ½Ã½ºÅÛ µî T7 promoter¸¦ ÀÌ¿ëÇÑ ¹ßÇö ½Ã½ºÅÛ¿¡´Â »ç¿ëÇÒ ¼ö ¾ø´Ù.
ÁÖ) º» Á¦Ç°Àº Electroporation¿¡´Â »ç¿ëÇÒ ¼ö ¾ø½À´Ï´Ù.
±¸¼º

1. TaKaRa Competent Cells BL21

100 §¡¡¿ 10°³

2. pUC19 DNA (0.1 ng/§¡)

10 §¡¡¿ 1°³

3. SOC Medium*

1 §¢¡¿ 10°³


* SOC Medium Á¶¼º

2 %

Tryptone

0.5 %

Yeast extract

10 mM

NaCl

2.5 mM

KCl

10 mM

MgSO4

10 mM

MgCl2

20 mM

Glucose


[ÁÖÀÇ]
SOC MediumÀ» ³ìÀÏ ¶§ °£È¤ ħÀüÀÌ »ý±â´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
ÀÌ·¯ÇÑ °æ¿ì, ½Ç¿Â¿¡¼­ Àß ¼¯°Å³ª 37¡É¿¡¼­ ¸î ºÐ°£ µ¥¿ö¼­ ħÀü¹°À» ³ìÀÎ ÈÄ »ç¿ëÇϼ¼¿ä.
º¸Á¸
-80¡É
Genotype
E.coli BL21£ºF£­, ompThsdS B (rB£­ mB£­), galdcm
ÇüÁú Àüȯ È¿À²
º» Á¦Ç° 100 §¡¿¡ 1 ngÀÇ pUC19 DNA¸¦ ÇüÁúÀüȯ Çϸé >1¡¿106 colonies/1 ¥ìg pUC19 DNA ÀÇ È¿À²À» ¾òÀ» ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- °íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ : pCold¢â Vector ½Ã¸®Áî
- Â÷¼¼´ë Membrane ±â¼úÀÇ His-tagged protein Á¤Á¦ column : Capturem¢â His-Tagged Purification
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî

Keyword :

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
>
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
>
ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
>
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
>
Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
>
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
>
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
>
¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)