´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã½ºÅÛ

flashBAC¢â Baculovirus Expression System

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6115
flashBAC¢â Baculovirus Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 reactions
°¡°Ý¹®ÀÇ   flashbac-baculovirus-expression-systems-protocol.pdf
Mirus
MIR 6120
flashBAC¢â Baculovirus Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 reactions
°¡°Ý¹®ÀÇ  
Mirus
MIR 6135
flashBAC¢â ULTRA Baculovirus Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 reactions
°¡°Ý¹®ÀÇ   flashbac-baculovirus-expression-systems-protocol.pdf
Mirus
MIR 6140
flashBAC¢â ULTRA Baculovirus Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 reactions
°¡°Ý¹®ÀÇ  
Mirus
MIR 6150
pOET1 Transfer Plasmid
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 §¡
(500 ng/§¡)
°¡°Ý¹®ÀÇ   poet1-transfer-plasmid-pds.pdf
Mirus
MIR 6151
pOET1C-6xHis Transfer Plasmid
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 §¡
(500 ng/§¡)
°¡°Ý¹®ÀÇ   poet1c-6xhis-transfer-plasmid-pds.pdf
Mirus
MIR 6152
pOET6 BacMam Transfer Plasmid
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 §¡
(500 ng/§¡)
°¡°Ý¹®ÀÇ   poet6-bacmam-transfer-plasmid-pds.pdf



Á¦Ç° ¼³¸í
flashBAC¢â Baculovirus Expression SystemÀº baculovirus¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛÀ¸·Î, baculovirus DNA¿Í control transfer plasmid·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
º» ½Ã½ºÅÛÀº Autographa californica multiple nucleopolyhedrovirus (AcMNPV)ÀÇ genome¿¡¼­ Çʼö À¯ÀüÀÚ ORF1629¸¦ °á½Ç½ÃÄÑ °ïÃæ¼¼Æ÷³»¿¡¼­ ÀçÁ¶ÇÕµÇÁö ¾ÊÀº virus°¡ º¹Á¦µÇÁö ¾ÊÀ¸¹Ç·Î, plaque assay¸¦ ÁøÇàÇÏÁö ¾Ê°íµµ ÀçÁ¶ÇÕµÈ virus¸¸À» ¾òÀ» ¼ö ÀÖ´Ù.
¶ÇÇÑ Chitinase gene (chiA)¸¦ Á¦°ÅÇÏ¿© ºÐºñÇü ´Ü¹éÁúÀ̳ª ¸·´Ü¹éÁúÀ» ¸Å¿ì È¿À²ÀûÀ¸·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù.
flashBAC¢â ULTRA Baculovirus Expression System (Code MIR 6135, MIR 6140)´Â ÇʼöÀûÀÌÁö ¾ÊÀº À¯ÀüÀÚ¸¦ Á¦°ÅÇÔÀ¸·Î½á ÀçÁ¶ÇÕ ´Ü¹éÁúÀÇ ¹ßÇö¾çÀ» ³ôÀÎ ¾÷±×·¹ÀÌµå ½Ã½ºÅÛÀÌ´Ù.
ÀÌ ½Ã½ºÅÛÀº AcMNPV polyhedrin À¯ÀüÀÚ ºÎÀ§¿¡ bacterial artificial chromosome (BAC)ÀÌ »ðÀԵǾî ÀÖ´Ù.
Á¦Ç° ¼±ÅÃ

System

Description

flashBAC¢â

The original flashBAC¢â expression vector. Protein expression and secretion is improved by deletion of the chitinase gene from the AcMNPV genome. Optimal for expression of most nuclear or cytoplasmic proteins.

flashBAC¢â ULTRA

The most advanced flashBAC¢â vector system, optimized by deletions in the chitinase, cathepsin, p10, p74 and p26 genes. The ideal system for the most difficult to express proteins including nuclear, cytoplasmic, membrane and secreted proteins.


flashBAC¢â SystemÀ¸·Î ÀçÁ¶ÇÕ baculovirus¸¦ Á¦ÀÛÇϱâ À§Çؼ­´Â insect cell, transfer plasmid, transfection reagent°¡ ÇÊ¿äÇÏ´Ù.
MirusÀÇ transfer vector (pOET1, pOET1C-6xHis, pOET6 BacMam) ¿Ü¿¡µµ ´Ù¾çÇÑ transfer vector¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ°í (ȣȯ °¡´ÉÇÑ transfer vector list º¸±â), TransIT¢ç-Insect Transfection Reagent (Code MIR 6104)·Î ³ôÀº ´Ü¹éÁú ¹ßÇöÀ» À¯µµÇÒ ¼ö ÀÖ´Ù.


±×¸². flashBAC¢â System overview
flashBAC¢â Baculovirus Expression System ±¸¼º*

Code

Product Name

Components

Concentration

Volume

MIR 6115

flashBAC¢â 5 reaction

flashBAC¢â DNA

20 ng/¥ìl

25 ¥ìl

Control Transfer Plasmid (pAcRP23-lacZ)

100 ng/¥ìl

5 ¥ìl

MIR 6120

flashBAC¢â 24 reaction

flashBAC¢â DNA

20 ng/¥ìl

120 ¥ìl

Control Transfer Plasmid (pAcRP23-lacZ)

100 ng/¥ìl

25 ¥ìl

MIR 6135

flashBAC¢â ULTRA 5 reaction

flashBAC¢â ULTRA DNA

20 ng/¥ìl

25 ¥ìl

Control Transfer Plasmid (pAcRP23-lacZ)

100 ng/¥ìl

5 ¥ìl

MIR 6140

flashBAC¢â ULTRA 24 reaction

flashBAC¢â ULTRA DNA

20 ng/¥ìl

120 ¥ìl

Control Transfer Plasmid (pAcRP23-lacZ)

100 ng/¥ìl

25 ¥ìl

º¸Á¸
flashBAC DNA: 4¡É
Control Transfer Plasmid (pAcRP23-lacZ): 4¡É ¶Ç´Â -20¡É
°ü·Ã Á¦Ç°
- Insect cellÀü¿ë transfection ½Ã¾à: TransIT¢ç-Insect Transfection Reagent
- All-in-One Baculovirus ¹ßÇö ½Ã½ºÅÛ: BacPAK¢â Baculovirus Expression System
- Baculovirus ¿ª°¡ÃøÁ¤: BacPAK¢â qPCR Titration Kit

Keyword :

°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
>
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
>
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
>
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
>
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
>
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
>
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
>
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
>
Plaque Assay ¼øÈ­°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
>
Sf9, Sf21 cell¿¡ ÃÖÀûÈ­µÈ Transfection reage..
ProFectin
>
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ­ È¿À² Çâ»ó
ProFold Baculovirus Vectors
>
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
>
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors