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TaKaRa
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pUC18/19 type vector

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3218
pUC18 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
101,000¿ø  3218_DS.v2109Da.pdf
Takara
3219
pUC19 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
101,000¿ø  3219_DS.v2109Da.pdf
Takara
3298
pHSG298 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
83,000¿ø  3298_DS.v2109Da.pdf
Takara
3299
pHSG299 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
83,000¿ø  3299_DS.v2109Da.pdf
Takara
3396
pHSG396 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
100,000¿ø  3396_DS.v2109Da.pdf
Takara
3398
pHSG398 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
(0.5 OD)
100,000¿ø  3398_DS.v2109Da.pdf

³óµµ : 250~1,000 §¶/ §¢
º¸Á¸ -20¡É
GenBank
Entry Name Accession No.
pUC18 SYNPUC18CV L09136
pUC19 SYNPUC19CV L09137
pHSG299 SYNHSG299 M19415
pHSG399 SYNHSG399 M19087*

1. pHSG298 DNA (Code 3298)´Â MCS¼­¿­ ÀÌ¿Ü¿¡´Â pHSG299 DNA¿Í °°´Ù. ´Ù¸¸, 1,446 À§Ä¡ÀÇ ¡®G¡¯°¡ °á¼ÕµÇ¾î ÀÖ´Ù. pHSG299 DNA (Code 3299)´Â GenBank¿¡ µî·ÏµÇ¾î ÀÖ´Â pHSG299 ¼­¿­°ú ¾Æ·¡ ¹è¿­¿¡ Â÷ÀÌ°¡ ÀÖ´Ù.
1633C, 2667-2668 GA¡æ°á¼Õ 1808C¡æT, 2228-2229 AT¡æTA, 2474G¡æA
2. pHSG396 ¹× pHSG398Àº multi-cloning siteÀÇ ¼­¿­ ÀÌ¿Ü´Â pHSG399¿Í µ¿ÀÏÇÏ´Ù.

ÁÖ) GenBank¿¡ µî·ÏµÇ¾î ÀÖ´Â pUC18¼­¿­Àº 1308¹øÀÌ GÀÌÁö¸¸, ´ç»ç¿¡¼­ ÆǸÅÁßÀÎ pUC18 º¤ÅÍ´Â 1308¹øÀÌ AÀÌ´Ù.
DNA ±æÀÌ
pUC18/pUC19 : 2,686 bp
pHSG298, pHSG299 : 2,676 bp
pHSG396 : 2,238 bp
pHSG398 : 2,227 bp
Á¦Ç°¼³¸í
pUC18/pUC19´Â dideoxy¹ý¿¡ ÀÇÇÑ DNA ¿°±â¼­¿­ °áÁ¤¿¡ ÀûÇÕÇÑ plasmid vector·Î ampicillin ³»¼ºÀ» °¡Á® M13 phage vector¿¡ ºñ±³ÇÏ¿© Å« DNA ´ÜÆíÀ» cloningÇÒ ¼ö ÀÖ´Ù. lacZ ¿µ¿ª¿¡ multi-cloning site¸¦ °®°í ÀÖ¾î IPTG¿Í X-galÀ» ÇÔÀ¯ÇÏ´Â plate¿¡¼­ ¿Ü·¡ DNAÀÇ µµÀÔ À¯¹«¸¦ °£´ÜÈ÷ ÆǺ°ÇÒ ¼ö ÀÖ´Ù. ¶Ç lac promoter¸¦ ÀÌ¿ëÇÑ ¿Ü·¡ À¯ÀüÀÚÀÇ ¹ßÇöµµ °¡´ÉÇÏ´Ù.
DNA sequencing¿¡´Â M13 primers¸¦ »ç¿ëÇÏ´Â °ÍÀÌ Æí¸®ÇÏ´Ù. ¶Ç Kilo-Sequence¿ë Deletion Kit (Code 6030)¸¦ »ç¿ëÇÏ¿© kilo-sequencingµµ °¡´ÉÇÏ´Ù.
pHSG298°ú pHSG299´Â kanamycin ³»¼ºÀ», pHSG396, pHSG398Àº chloramphenicol ³»¼ºÀ» °¢°¢ÀÇ ¼±Åà ¸¶Ä¿·Î °®´Â pUC type cloning vector plasmidÀÌ´Ù.
pHSG298°ú pHSG398Àº pUC18°ú, pHSG299´Â pUC19¿Í µ¿ÀÏÇÑ multi-cloning site¸¦ °®°í ÀÖ´Ù (´Ü, pHSG298°ú pHSG299´Â Hind III, Sma I ºÎÀ§¸¦ »ç¿ëÇÒ ¼ö ¾ø´Ù). ¶Ç pHSG396Àº pHSG398°ú ´Ù¸¥ multi-cloning site¸¦ °¡Áö°í ÀÖ´Ù.
¿ëµµ
- ¿Ü·¡ À¯ÀüÀÚÀÇ ¥á- »óº¸¼ºÀ» ÀÌ¿ëÇÑ Å¬·Î´×°ú lac promoter¸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚ ¹ßÇö
- M13 primers¸¦ ÀÌ¿ëÇÑ DNA ¿°±â¼­¿­ °áÁ¤
- Kilo-Sequence¿ë Deletion Kit (TaKaRa Code 6030)¸¦ ÀÌ¿ëÇÑ ±ä ´ÜÆíÀÇ ¿°±â¼­¿­ °áÁ¤
»ç¿ë»óÀÇ ÁÖÀÇ
pHSG298°ú pHSG299 ÁßÀÇ Stu¥°Àνļ­¿­Àº Stu¥°À¸·Î Àý´ÜÇÒ ¼ö ¾ø´Ù. ¼­¿­¿¡´Â ¹®Á¦°¡ ¾ø±â ¶§¹®¿¡ ±¸Á¶»óÀÇ ¹®Á¦·Î »ý°¢µÈ´Ù.
°ü·ÃÁ¦Ç°
M13 primers (Code 3810, 3820, 3830A, 3830B, 3831, 3832A, 3832B)


±×¸². pUC18/ pUC19 DNAÀÇ ±¸Á¶

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